The genome structure of ciprofloxacin-resistant Mycoplasma hominis clinical isolates

Kolesnikova, E. A.; Бруснигина, Н. Ф.; Makhova, Maria A.; Алексеева, А. Е.

doi:10.32607/actanaturae.10941

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…It has been suggested that the resistance of M. hominis to ciprofloxacin is the result of the efflux transporters of MATE family. There are only a few reports that such efflux pumps can transport macrolides (13,14).…”

Section: Introductionmentioning

confidence: 99%

Emergence and Mechanism of Resistance of Tulathromycin Against Mycoplasma hyopneumoniae in a PK/PD Model and the Fitness Costs of 23S rRNA Mutants

et al. 2022

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Macrolides are widely used in diseases caused by Mycoplasma spp. The new semi-synthetic macrolide antibiotic tulathromycin is currently in wide use for the treatment of respiratory diseases of livestock. The objective of this study was to evaluate the antibacterial effect of tulathromycin against Mycoplasma hyopneumoniae using an in vitro pharmacokinetic/pharmacodynamic (PK/PD) model to reveal mechanisms of antibiotic resistance and to evaluate the fitness of drug-resistant strains. In this study, high performance liquid chromatography-tandem mass spectrometry was used to determine drug concentrations for the in vitro model after dosing. The peak concentrations were in the range 0.3125–20 μg/mL (1 × MIC-64 × MIC). The ratio of the area under the concentration-time curve (AUC) over 72 h divided by the MIC (AUC72h/MIC) had the highest correlation with the antibacterial effect of tulathromycin against M. hyopneumoniae. Tulathromycin also showed concentration-dependent antimicrobial effects and promoted the emergence of drug-resistant bacteria after being cultured for 168 h and most were mutations in 23S rRNA at site A2058G (E.coli numbering) and only a single isolate was an A2058T (E.coli numbering) mutant. In the presence of reserpine, we determined the MIC of tulathromycin, tilmicosin, tiamulin and tylosin against these drug-resistant bacteria and the strains with efflux pump mechanisms were found among the strains resistant to tilmicosin. Gene expression analysis indicated that the ABC and MATE transporter efflux pump genes RS01935, RS02670, RS01115, RS01970, RS02395 and RS03540 (MATE family efflux transporter) were up-regulated in the three strains (P < 0.05 or P < 0.01). These investigations provide guidance for clinical administration of tulathromycin and elucidate the mechanism and fitness cost of drug resistance in M. hyopneumoniae.

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Section: Introductionmentioning

confidence: 99%

Emergence and Mechanism of Resistance of Tulathromycin Against Mycoplasma hyopneumoniae in a PK/PD Model and the Fitness Costs of 23S rRNA Mutants

et al. 2022

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Multi-locus sequence typing of geographically and temporally diverse strains of Mycoplasma hominis

Boujemaa,

Suri,

Kaur

2024

Diagnostic Microbiology and Infectious Disease

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Capabilities of combined peptide drugs in the correction of male infertility (literature review)

Prokopiev

Abdrakhmanov

2021

Consilium Medicum

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Acute and chronic urogenital infections are associated with male infertility. Besides "traditional" sexually transmitted infections, there is a significant increase in inflammatory diseases of reproductive system caused by opportunistic pathogens. Studies show that sperm fertility is influenced by direct exposure to sexually transmitted infections, as well as dysfunction of the blood-testicular barrier, leading to autoimmune infertility. In a number of clinical trials, Prostatilen AC was shown to have benefitial effects on the ejaculate.

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The genome structure of ciprofloxacin-resistant Mycoplasma hominis clinical isolates

Cited by 4 publications

References 12 publications

Emergence and Mechanism of Resistance of Tulathromycin Against Mycoplasma hyopneumoniae in a PK/PD Model and the Fitness Costs of 23S rRNA Mutants

Emergence and Mechanism of Resistance of Tulathromycin Against Mycoplasma hyopneumoniae in a PK/PD Model and the Fitness Costs of 23S rRNA Mutants

Multi-locus sequence typing of geographically and temporally diverse strains of Mycoplasma hominis

Capabilities of combined peptide drugs in the correction of male infertility (literature review)

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