The genomic architecture of mastitis resistance in dairy sheep

Banos, Georgios; Bramis, G.; Bush, Stephen J.; Clark, Emily L.; McCulloch, Mary E. B.; Smith, Jacqueline; Schulze, G; Arsenos, G.; Hume, David; Psifidi, Androniki

doi:10.1186/s12864-017-3982-1

Cited by 56 publications

(63 citation statements)

References 72 publications

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“…Candidate genes implicated in innate immunity and thus resistance to mastitis included SOCS2 , CTLA4 , C6 , C7 , C9 , PTGER4 , DAB2 , CARD6 , OSMR , PLXNC1 , IDH1 , ICOS , FYB and LYFR in Chios breed (Banos et al, ). Fragkou et al () have attributed the breed differences in susceptibility of mastitis to differences in lymphocyte activity and proposed a potential genetic background to the infection, given that in cattle, the genetics of bovine leucocyte antigen ( BoLA ) has been linked to resistance to mastitis (Takeshima & Aida, ).…”

Section: Animal‐related Predisposing Factorsmentioning

confidence: 99%

Predisposing factors for bacterial mastitis in ewes

Vasileiou

Mavrogianni

Petinaki

et al. 2019

Reprod Domestic Animals

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Objective of this review paper is the appraisal of predisposing factors for bacterial mastitis in ewes. Factors that predispose ewes to mastitis can be classified into non‐animal‐related factors (environmental and climatological factors, housing, nutrition, milking practices) and animal‐related factors (anatomic, genetic, litter size—ethological factors, number and stage of lactation period, health problems). There are clear management—environment—animal interactions in mastitis development in ewes, which underline its multifactorial nature. Research and studies regarding risk factors are important, in order to develop strategies for their elimination, control or correction. Control measures for bacterial mastitis, which attempt to eliminate predisposing factors for infection, can thus be developed and implemented.

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Section: Animal‐related Predisposing Factorsmentioning

confidence: 99%

Predisposing factors for bacterial mastitis in ewes

Vasileiou

Mavrogianni

Petinaki

et al. 2019

Reprod Domestic Animals

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“…When located on the same chromosomes, the significant markers identified for milk yield were in linkage disequilibrium (LD=0.27-0.97), implying that they correspond to the same causative mutation (S1 Table). The significant SNPs identified in the present study were not in LD with the SNPs previously associated with the mastitis related traits in Chios sheep [10] (S1 Table). Only small LD blocks were visualised with Haploview, probably due to a high number of recombination events having taken place in the outbred population of study.…”

Section: Resultsmentioning

confidence: 67%

“…Total number of individual animal records collected amounted to 2,436. Animal records for clinical mastitis occurrence and three mastitis indicator traits (milk somatic cell count, California Mastitis Test score and total viable bacterial count in milk) were also collected at the time of these visits by a qualified veterinarian, as described previously [10]. The three mastitis indicator traits may capture subclinical mastitis incidences and reflect the general health status of the udder.…”

Section: Methodsmentioning

confidence: 99%

“…For the design of this custom-made array, SNPs were selected from 50K and 800K SNP ovine DNA arrays, as well as available re-sequencing data. The average density of the array was 1 SNP every 23 Kb (for more details see [10]). This genomic tool was built within an FP7 European research project (http://cordis.europa.eu/result/rcn/163471_en.html).…”

Section: Methodsmentioning

confidence: 99%

“…Host resistance to mastitis is a moderately heritable trait [7]. Recently, an ovine custom made mastitis specific 960-SNP DNA array was built to facilitate genetic selection and improvement of animal resistance to mastitis in dairy sheep [10] [11] [12] [13]. We previously used this array in a genomic association study and detected five quantitative trait loci (QTLs) for mastitis resistance in Chios sheep [10].…”

Section: Introductionmentioning

confidence: 99%

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Genomic analyses underpin the feasibility of concomitant genetic improvement of milk yield and mastitis resistance in dairy sheep

Banos

Clark

Bush

et al. 2019

Preprint

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24Milk yield is the most important dairy sheep trait and constitutes the key genetic improvement 25 goal via selective breeding. Mastitis is one of the most prevalent diseases, significantly 65 Page4 66Beyond simply increasing milk production, the dairy sheep industry faces challenges such as 67 the need to offer healthy products to consumers, addressing animal welfare, and ensuring the 68 long-term competitiveness and sustainability of the sector. Mastitis is the most prevalent and 69 costly disease in the dairy industry due to reduced and discarded milk, early involuntary culling 70 of animals, and veterinary services and labour costs [6, 7]. The disease also poses a potential 71 threat of zoonosis and antimicrobial resistance if antibiotic treatment is not applied carefully 72 [6][7][8]. Moreover, mastitis is a welfare concern because of associated pain, anxiety and 73 restlessness, and upsets the normal feeding behaviour of the animals [9]. Host resistance to 74 mastitis is a moderately heritable trait [7]. Recently, an ovine custom made mastitis specific 75 960-SNP DNA array was built to facilitate genetic selection and improvement of animal 76 resistance to mastitis in dairy sheep [10] [11] [12] [13]. We previously used this array in a 77 genomic association study and detected five quantitative trait loci (QTLs) for mastitis 78 resistance in Chios sheep [10]. 80In the present study, we examined the genetic and genomic relationship between milk yield 81 and mastitis resistance in the Chios sheep, using pedigree and genomic information. The 82 relationship between the two traits is crucial if mastitis resistance is to be included in selective 83 breeding goals together with increased milk yield. We estimated genetic parameters and 84 investigated whether relevant QTLs for milk yield exist in previously identified mastitis-85 specific genomic regions. We also performed pathway analysis and examined gene expression 86 and allelic expression imbalance to characterise the genes located under the QTL regions in 87 relation with milk yield and mastitis resistance. 88 Page5 89 Materials and Methods 90 91 Ethical statement 92 The study was approved by the Ethics and Research Committee of the Faculty of Veterinary 93 Medicine, Aristotle University of Thessaloniki, Greece. Permits for access and use of the 94 commercial farms were granted by the farm owners, who were members of the Chios Sheep 95 Breeders' Cooperative "Macedonia". During sampling, animals were handled by qualified 96 veterinarians. Permission to qualified veterinarians to perform milk and blood sampling was 97 granted by the National (Greek) Legislature for the Veterinary Profession, No. 344/29-12-98 2000. 99 100 Animals, sampling and phenotyping 101 Animals used in the present study included 609 purebred Chios dairy ewes raised in four 102 commercial farms in Greece. Complete pedigree data were available for these animals. Ewes 103 were in their first or second lactation. Daily milk yield was recorded on each animal on the day 104 of monthly visits t...

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Genomic diversity and population structure of three autochthonous Greek sheep breeds assessed with genome-wide DNA arrays

et al. 2018

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In the present study, genome-wide genotyping was applied to characterize the genetic diversity and population structure of three autochthonous Greek breeds: Boutsko, Karagouniko and Chios. Dairy sheep are among the most significant livestock species in Greece numbering approximately 9 million animals which are characterized by large phenotypic variation and reared under various farming systems. A total of 96 animals were genotyped with the Illumina's OvineSNP50K microarray beadchip, to study the population structure of the breeds and develop a specialized panel of single-nucleotide polymorphisms (SNPs), which could distinguish one breed from the others. Quality control on the dataset resulted in 46,125 SNPs, which were used to evaluate the genetic structure of the breeds. Population structure was assessed through principal component analysis (PCA) and admixture analysis, whereas inbreeding was estimated based on runs of homozygosity (ROHs) coefficients, genomic relationship matrix inbreeding coefficients (F) and patterns of linkage disequilibrium (LD). Associations between SNPs and breeds were analyzed with different inheritance models, to identify SNPs that distinguish among the breeds. Results showed high levels of genetic heterogeneity in the three breeds. Genetic distances among breeds were modest, despite their different ancestries. Chios and Karagouniko breeds were more genetically related to each other compared to Boutsko. Analysis revealed 3802 candidate SNPs that can be used to identify two-breed crosses and purebred animals. The present study provides, for the first time, data on the genetic background of three Greek indigenous dairy sheep breeds as well as a specialized marker panel that can be applied for traceability purposes as well as targeted genetic improvement schemes and conservation programs.

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The genomic architecture of mastitis resistance in dairy sheep

Cited by 56 publications

References 72 publications

Predisposing factors for bacterial mastitis in ewes

Predisposing factors for bacterial mastitis in ewes

Genomic analyses underpin the feasibility of concomitant genetic improvement of milk yield and mastitis resistance in dairy sheep

Genomic diversity and population structure of three autochthonous Greek sheep breeds assessed with genome-wide DNA arrays

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