The genuine localization of indole alkaloids in Vinca minor and Catharanthus roseus

Abouzeid, Sara; Hijazin, Tahani; Lewerenz, Laura; Hänsch, Robert; Selmar, Dirk

doi:10.1016/j.phytochem.2019.112110

Cited by 10 publications

(4 citation statements)

References 44 publications

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“…The co-accumulation of catharanthine and vindoline in idioblasts observed in this study challenges previous data suggesting that catharanthine is accumulated at the epidermal cuticle, away from the idioblast-accumulated vindoline ( Roepke et al , 2010 ; Yu and De Luca, 2013 ). However, the claimed specific accumulation of catharanthine in the leaf surface was based on a leaf chloroform dipping technique, which was later shown to destroy the cell integrity of C. roseus leaves, leading to alkaloid cell leakage ( Abouzeid et al , 2019 ). Moreover, previous work by the same research group, using an uncontested abrasion technique to isolate the epidermis/cuticle, had shown that catharanthine was accumulated in the remnant leaf body ( Murata and De Luca, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

The leaf idioblastome of the medicinal plantCatharanthus roseusis associated with stress resistance and alkaloid metabolism

Guedes,

Ribeiro,

Carqueijeiro

et al. 2023

Journal of Experimental Botany

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Catharanthus roseus leaves produce a range of monoterpenoid indole alkaloids (MIAs) that include low levels of the anticancer drugs vinblastine and vincristine. The MIA pathway displays a complex architecture spanning different subcellular and cell-type localizations and is under complex regulation. As a result, the development of strategies to increase the levels of the anticancer MIAs has remained elusive. The pathway involves mesophyll specialised idioblasts where the late unsolved biosynthetic steps are thought to occur. Here, protoplasts of C. roseus leaf idioblasts were isolated by fluorescence-activated cell sorting, and their differential alkaloid and transcriptomic profiles were characterised. This involved the assembly of an improved C. roseus transcriptome from short- and long-read data, IDIO+. It was observed that C. roseus mesophyll idioblasts possess a distinctive transcriptomic profile associated with protection against biotic and abiotic stresses, and indicative that this cell type is a carbon sink, in contrast with surrounding mesophyll cells. Moreover, it is shown that idioblasts are a hotspot of alkaloid accumulation, suggesting that their transcriptome may hold the keys to the in-depth understanding of the MIA pathway and the success of strategies leading to higher levels of the anticancer drugs.

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Section: Discussionmentioning

confidence: 99%

The leaf idioblastome of the medicinal plantCatharanthus roseusis associated with stress resistance and alkaloid metabolism

Guedes,

Ribeiro,

Carqueijeiro

et al. 2023

Journal of Experimental Botany

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“…The development of the 12 biocomposites (P1-P12) for topical administration was based on the numerous benefits that the indole compounds extracted from the Vinca minor plant demonstrated for dermal application [26][27][28]. The composition of these preparations was formulated to ensure biocompatibility, adhesion, and a possible improved therapeutic effect.…”

Section: Discussionmentioning

confidence: 99%

Development of New Dermato-Cosmetic Therapeutic Formulas with Extracts of Vinca minor L. Plants from the Dobrogea Region

Neculai,

Stanciu,

Lepădatu

et al. 2023

IJMS

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A new trend in the use of indole alkaloids from natural products is the preparation of topical pharmaceutical formulations with applications in the field of regenerative medicine. These formulations can be characterized through the ease of administration, the proven healing action of indole alkaloids, the protection of skin lesions, and the assurance of oxygen permeability. Based on the numerous benefits that indole compounds extracted from the Vinca minor plant show externally, the purpose of this study was to develop new semi-solid biocomposites for topical application obtained from hydroalcoholic macerates of 40%, 70%, and 96% concentrations from the stems and leaves of the Vinca minor L. plant from the Dobrogea area. A total of 12 pharmaceutical formulations (named P1–P12) were prepared for which the physicochemical properties, pH, thermal stability, spreading capacity, and rheological behavior were determined. The optimal formulas with antioxidant and antimicrobial capacity were evaluated and determined (P3, P4, P9, and P10). Antioxidant activity was elicited using the photochemiluminescence method. The microorganisms used for the evaluation of antimicrobial activity were Gram-positive Staphylococcus aureus (ATCC 25923), Gram-negative Escherichia coli (ATCC 25922), and a fungal species, Candida albicans (ATCC 900288). The study of the rheological profile for the obtained composites revealed Newtonian, pseudoplastic, and thixotropic fluid behaviors. Following determinations using the photochemiluminescence method, the best antioxidant activity was obtained in the P3 and P9 preparations. The results of the antimicrobial analysis confirmed that both the leaves and the stems of the Vinca minor plant represent a valuable source of antibacterial substances, and the biocomposites analyzed may represent an alternative in the realization of new pharmaceutical preparations with topical applications based on hydroalcoholic macerates obtained from the Vinca minor plant.

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“…These plants had been intensely studied for their therapeutic properties [16,17], due to the rich alkaloid content [18][19][20][21]. Moreover, a relation between the morphological and ultrastructural aspects of the leaf in Vinca species, and the chemical composition is yet to be established [22].…”

Section: Introductionmentioning

confidence: 99%

The Phytochemical Analysis of Vinca L. Species Leaf Extracts Is Correlated with the Antioxidant, Antibacterial, and Antitumor Effects

et al. 2021

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The phytochemical analysis of Vinca minor, V. herbacea, V. major, and V. major var. variegata leaf extracts showed species-dependent antioxidant, antibacterial, and cytotoxic effects correlated with the identified phytoconstituents. Vincamine was present in V. minor, V. major, and V. major var. variegata, while V. minor had the richest alkaloid content, followed by V. herbacea. V. major var. variegata was richest in flavonoids and the highest total phenolic content was found in V. herbacea which also had elevated levels of rutin. Consequently, V. herbacea had the highest antioxidant activity followed by V. major var. variegata. Whereas, the lowest one was of V. major. The V. minor extract showed the most efficient inhibitory effect against both Staphylococcus aureus and E. coli. On the other hand, V. herbacea had a good anti-bacterial potential only against S. aureus, which was most affected at morphological levels, as indicated by scanning electron microscopy. The Vinca extracts acted in a dose-depended manner against HaCaT keratinocytes and A375 melanoma cells and moreover, with effects on the ultrastructure, nitric oxide concentration, and lactate dehydrogenase release. Therefore, the Vinca species could be exploited further for the development of alternative treatments in bacterial infections or as anticancer adjuvants.

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The genuine localization of indole alkaloids in Vinca minor and Catharanthus roseus

Cited by 10 publications

References 44 publications

The leaf idioblastome of the medicinal plantCatharanthus roseusis associated with stress resistance and alkaloid metabolism

The leaf idioblastome of the medicinal plantCatharanthus roseusis associated with stress resistance and alkaloid metabolism

Development of New Dermato-Cosmetic Therapeutic Formulas with Extracts of Vinca minor L. Plants from the Dobrogea Region

The Phytochemical Analysis of Vinca L. Species Leaf Extracts Is Correlated with the Antioxidant, Antibacterial, and Antitumor Effects

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