The Genus Aspergillus, from Taxonomy and Genetics to Industrial Applications

Peterson, Stephen W.; Powell, Keith A.; Renwick, Annabel; Peberdy, John F.

doi:10.2307/3760893

Cited by 18 publications

(2 citation statements)

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“…A. niger is a filamentous fungus that is suitable for solid-state fermentation, because it can easily penetrate and colonize solid surfaces. More crucially, the US Food and Drug Administration has classified A. niger as "Generally Recognized as Safe", making it usable in the food industry [27]. Potato dextrose agar (PDA) media were used for the growth of the fungus.…”

Section: Isolation and Selection Of Microorganismmentioning

confidence: 99%

Laccase Production from Local Biomass Using Solid State Fermentation

et al. 2023

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The large family of enzymes, known as polyphenols oxidases, includes laccase. Due to the inclusion of a copper atom in their catalytic core, laccases are frequently referred to as multi-copper oxidases. Laccases are versatile enzymes that can catalyze the oxidation of a wide range of phenolic and non-phenolic substances. In the current study, a local strain of Aspergillus niger was used for solid-state fermentation to produce fungal laccase, as well as purify and optimize laccase. The enzyme profile, which was acquired using guaiacol to measure enzyme activity, showed that after five days of incubation, wheat straw provided the highest level of laccase activity, or 2.551 U/mL. A technique called response surface methodology (RSM) was used to examine the effects of various conditions on the production of enzymes. The RSM results demonstrated that after five days of incubation, the enzyme activity was at its highest at 45 °C, pH 5.5, and 30% moisture level, inoculated with 2 mL mycelium. Through ammonium sulphate precipitation and dialysis, the enzyme was purified. Additionally, column chromatography was used to further purify laccase. The next step was enzyme characterization to evaluate how temperature and pH affected enzyme activity. At 45 °C and pH 5.5, the isolated enzyme produced its highest level of activity. The findings of the current study showed that A. niger is capable of producing laccase in an economical and environmentally friendly way. Due to its unique oxidative and catalytic features, this enzyme has received a lot of attention recently.

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Section: Isolation and Selection Of Microorganismmentioning

confidence: 99%

Laccase Production from Local Biomass Using Solid State Fermentation

et al. 2023

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“…Few members of the genus are pathogenic to humans (Aspergillosis) (Karkowska-Kuleta et al, 2009;Brown et al, 2012) and their carcinogenic syndrome are indirectly caused by their metabolites (Gourama and Bullerman, 1995;Pitt et al, 2000;Bayman et al, 2002). Nevertheless, majority of Aspergillus have been reported to be beneficial in agricultural and industrial applications (Contesini et al, 2010;Powell et al, 2013). Some are important sources of industrial biodegradable enzymes (de Vries and Visser, 2001).…”

Section: B) Application Of Microbesmentioning

confidence: 99%

Occurrence, seasonal variation and management of tomato leafminer (tuta absoluta meyrick.) in Tanzania

Zekeya¹

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Tomato leafminer [Tuta absoluta, Meyrick. (Lepidoptera: Gelechiidae)] is a notorious pest that causes massive losses in Solanaceae’s crops including tomato (Solanum lycopersicum L.). This study was conducted to evaluate the occurrence, damage incidences, host range, population dynamics and developed bio-control strategies for managing T. absoluta in Tanzania. Field surveys were conducted in thirteen tomato production regions of Tanzania by purposively sampling one region and one district from each region then three villages were randomly selected for field survey. Optima lures was deployed per plot (0.5 acre) and number of adult moths trapped were counted after 7 days. To identify host of T. absoluta, in each selected village number and types of crops damaged per plot were recorded. Ten plants per plot were randomly selected to determine the rate of T. absoluta damage per plant by counting number of damaged leaves/fruits per plant in each plot and expressed as percentage damage. However, botanical extracts against T. absoluta larvae was conducted in laboratory by using four plant species extracted by using organic solvents. Different concentrations of extract were tested against 10 second instar larvae of T. absoluta that were selected randomly. Mortality rate, pupation rate and adult emergence rate were recorded at interval of 24h. Results indicated that T. absoluta was present in 13 regions surveyed and proliferates in 12 host plants including tomato, potato, nightshade, pepper and some weed species particularly Solanum incanum. Population density of T. absoluta was high in Iringa compared to Arusha and Morogoro and the dry season exhibited higher number of T. absoluta than in rainy season in all regions surveyed. The study on T. absoluta management revealed that, the pest can be controlled by using locally available entomopathogenic fungi (Patent No. TZ/P/2018/000035), plant extracts from Bersama abyssinica stem bark, Commiphora africana leaves, Tergetes minuta seeds and Moringa oleifera essential oil from seeds and cultivation of four accessions of tomato landraces namely BKM-22, KGM-5, HSK-4 and HSK-38. Application of the developed bio–control strategies caused 60% to 80% mortality of adults and larvae of T. absoluta in the laboratory and open field conditions. The identified bio-control strategies and resistance tomato landraces are recommended for further analysis and for consideration as remedies against T. absoluta in tomato in Tanzania.

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Effect of natural maize phytochemicals on Aspergillus section Flavi sclerotia characteristics under different conditions of growth media and water potential

Nesci

Etcheverry

2009

Fungal Ecology

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The Genus Aspergillus, from Taxonomy and Genetics to Industrial Applications

Cited by 18 publications

References 0 publications

Laccase Production from Local Biomass Using Solid State Fermentation

Laccase Production from Local Biomass Using Solid State Fermentation

Occurrence, seasonal variation and management of tomato leafminer (tuta absoluta meyrick.) in Tanzania

Effect of natural maize phytochemicals on Aspergillus section Flavi sclerotia characteristics under different conditions of growth media and water potential

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