The Genus Bdellovibrio

Stolp, H.

doi:10.1007/978-3-662-13187-9_54

Cited by 11 publications

(5 citation statements)

References 78 publications

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“…Based on Stolp (1981), a water sample or 50-g soil in 500 mL in sterile buffer is shaken vigorously for 1 h and then centrifuged for 5 min at 2,000 g to remove gross particles. The supernatant is passed through a series of membrane filters of decreasing pore size (3.0, 1.2, 0.8, and 0.45 mm).…”

Section: Direct Isolation Of Bdellovibrio From Environmental Samplesmentioning

confidence: 99%

Bdellovibrio and Like Organisms

2014

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Section: Direct Isolation Of Bdellovibrio From Environmental Samplesmentioning

confidence: 99%

Bdellovibrio and Like Organisms

2014

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“…Bdellovibrio bacteriovorus is a predatory bacterium that grows intraperiplasmically on other gram-negative bacteria (15,17). The bdellovibrio cell alternates between the two developmental stages of a free-living, motile attack phase and an intracellular, filamentous growth phase.…”

mentioning

confidence: 99%

Prey-derived signals regulating duration of the developmental growth phase of Bdellovibrio bacteriovorus

Gray

Ruby

1990

J Bacteriol

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The filamentous elongation typical of growth-phase cells of the predatory bacterium Bdelovibrio bateriovorus is mediated by regulatory signals that are derived from the prey cell itself. These signals regulate the differentiation of growth-phase cells into the attack phase and appear to be required for continued filamentous growth by prey-dependent wild-type bdellovibrios and their prey-independent mutant derivatives alike. Using a prey-independent bdellovibrio strain, we have developed an assay for the detection and quantification of the growth-extending signal activity present in extracts of prey cells. This prey-derived regulatory activity was shown to be independent of its nutritional contribution to the bdellovibrios and was found to occur in heat-stable, proteinlike compounds of a variety of native molecular weights within the soluble fraction of extracts from both gram-negative and gram-positive bacteria.

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“…The technique first described by Stolp and Starr (1963) for the direct isolation of Bdellovibrio from soil, and subsequently explained by Starr and Stolp (1976), Stolp (1981), and Ruby (1992), still works effectively. A 50-to 500-g soil sample is suspended in 500 ml of tap water or buffer.…”

Section: Direct Isolationmentioning

confidence: 97%

“…DNB provided the best results for all combinations. The use of an obliquely illuminated colony counter also assists in plaque visibility (Stolp 1981).…”

Section: Enumerationmentioning

confidence: 99%

The Search for Hunters: Culture-Dependent and -Independent Methods for Analysis of Bdellovibrio and Like Organisms

Koval

Predatory Prokaryotes

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The Genus Bdellovibrio

Cited by 11 publications

References 78 publications

Bdellovibrio and Like Organisms

Bdellovibrio and Like Organisms

Prey-derived signals regulating duration of the developmental growth phase of Bdellovibrio bacteriovorus

The Search for Hunters: Culture-Dependent and -Independent Methods for Analysis of Bdellovibrio and Like Organisms

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