The genus Diaporthe: a rich source of diverse and bioactive metabolites

Chepkirui, Clara; Stadler, Marc

doi:10.1007/s11557-017-1288-y

Cited by 79 publications

(73 citation statements)

References 113 publications

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“…In our research, ethyl acetate extracts of 13 endophytic fungi (10% of total screened strains) showed inhibitory activity against pathogenic microorganisms. Most of the 13 isolates represent fungal genera, including Aspergillus , Diaporthe , and Trichoderma , previously reported to produce antimicrobial compounds [ 25 , 26 , 27 ]. However, new bioactive compounds are constantly being discovered from plant endophytes.…”

Section: Discussionmentioning

confidence: 99%

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Shi

et al. 2020

IJMS

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The plant Chloranthus japonicus Sieb is known for its anticancer properties and mainly distributed in China, Japan, and Korea. In this study, we firstly investigated the diversity and antimicrobial activity of the culturable endophytic fungi from C. japonicus. A total of 332 fungal colonies were successfully isolated from 555 tissue segments of the medicinal plant C. japonicus collected from Qinling Mountains, China. One hundred and thirty representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), five classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Agaricomycetes, Mucoromycetes), and at least 30 genera. Colletotrichum (RA, 60.54%) was the most abundant genus, followed by Aspergillus (RA, 11.75%) and Diaporthe (RA, 9.34%). The Species Richness Index (S, 56) and the Shannon-Wiener Index (H′, 2.7076) indicated that C. japonicus harbored abundant fungal resources. Thirteen out of 130 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Among of these, F8158, which was identified as Trichoderma cf. harzianum, exhibited good antagonistic capacities (the percent inhibition of mycelial growth ranged from 47.72~88.18) for different pathogens and has a potential application in biological control. In addition, it is noteworthy that the strain F8157 (Thanatephorus cucumeris, an opportunistic pathogen) showed antibacterial and antifungal activity, which is reported firstly in this study, and should be investigated further. Taken together, these results indicated that the endophytic fungi from C. japonicus may be of potential interest in screening bio-control agents and discovering of new bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Shi

et al. 2020

IJMS

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“…This compound was first reported from a marine-derived fungus that was originally assigned to the genus Phomopsis (Kobayashi et al 2003). However, it has since then been found in other, terrestrial strains of the same genus, which should now be referred to as Diaporthe (Chepkirui and Stadler 2017), a large genus of the order Diaporthales. Interestingly, phomopsidin derivatives have never been reported from cultures of Xylariales before Halecker et al (2020) found the compound in dual antagonist assays in agar cultures as described above.…”

Section: Introductionmentioning

confidence: 98%

Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture

Pourmoghaddam

Lambert

Surup

et al. 2020

Self Cite

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During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosumsensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen.

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“…Diaporthe species are widespread, and well-known as causal agents of many important plant diseases, including root and fruit rots, dieback, stem cankers, leaf spots, leaf and pod blights and seed decay ( Uecker 1988 , Mostert et al 2001a , b , Van Rensburg et al 2006 , Rehner & Uecker 1994 , Santos et al 2011 , Udayanga et al 2011 , Tan et al 2013 ). Species of the genus have also been used in secondary metabolite research due to their production of a large number of polyketides and a variety of unique low- and high-molecular-weight metabolites with different antibacterial, anticancer, antifungal, antimalarial, antiviral, cytotoxic and herbicidal activities ( Corsaro et al 1998 , Isaka et al 2001 , Dai et al 2005 , Kumaran & Hur 2009 , Yang et al 2010 , Gomes et al 2013 , Chepkirui & Stadler 2017 ), and for biological control of fungal pathogens ( Santos et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe

Guarnaccia¹,

Groenewald²,

Woodhall³

et al. 2018

persoonia

126

133

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Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre- or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on V. vinifera plants, and provides useful information for effective disease management.

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The genus Diaporthe: a rich source of diverse and bioactive metabolites

Cited by 79 publications

References 113 publications

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture

Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe

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