The genus name Ensifer Casida 1982 takes priority over Sinorhizobium Chen et al. 1988, and Sinorhizobium morelense Wang et al. 2002 is a later synonym of Ensifer adhaerens Casida 1982. Is the combination 'Sinorhizobium adhaerens' (Casida 1982) Willems et al. 2003 legitimate? Request for an Opinion

Young, J. M.

doi:10.1099/ijs.0.02665-0

Cited by 174 publications

(76 citation statements)

References 17 publications

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“…The genus Ensifer currently includes eleven species Wei et al, 2002;Young, 2003;Toledo et al 2003). Two further species, 'Ensifer mexicanus' and 'Sinorhizobium chiapanecum', have been described but the names have not yet been validly published (Lloret et al 2007;Rincó n-Rosales et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

Merabet

Martens

Mahdhi

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Nine isolates from Argyrolobium uniflorum, Lotus creticus, Medicago sativa (Tunisia) and Lotus arabicus (Senegal) were analysed by multilocus sequence analysis (MLSA) of five housekeeping genes (recA, atpD, glnA, gltA and thrC), the 16S rRNA gene and the nodulation gene nodA. Analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, A, B and C, all belonging to the branch of the genus Ensifer, regardless of the phylogenetic reconstruction method used (maximum likelihood, maximum-parsimony, neighbour-joining). The three groups were further characterized by API 100 auxanographic tests, host specificity and nodA gene sequence analysis. On the basis of these data, clusters A and C are suggested as representing two novel species within the genus Ensifer, for which the names Ensifer numidicus sp. nov. (type strain ORS 1407T=LMG 24690T=CIP 109850T) and Ensifer garamanticus sp. nov. (type strain ORS 1400T=LMG 24692T=CIP 109916T) are proposed. The cluster B strains were assigned to Ensifer adhaerens genomovar A.

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Section: Introductionmentioning

confidence: 99%

Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

Merabet

Martens

Mahdhi

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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“…Ensifer kummerowiae, Ensifer medicae, Ensifer meliloti, Ensifer mexicanus, Ensifer morelense, Ensifer numidicus, Ensifer psoraleae, Ensifer saheli, Ensifer sesbaniae, Ensifer sojae and Ensifer terangae (Casida Jr, 1982;Li et al, 2011;Lloret et al, 2007;Martens et al, 2008;Merabet et al, 2010;Wang et al, 2013;Yan et al, 2016;Young, 2003). In order to clarify the accurate taxonomic position of this novel Ensifer group, we performed the present study to compare it with the defined Ensifer species.…”

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confidence: 99%

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Yan

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A group of Sesbania cannabina rhizobia belonging to four recA genotypes of a novel group was further characterized in comparison with the related Ensifer species. They showed 98.2 to 99.9 % similarities among themselves and 92.9 to 93.3 % similarities with the most related strain Ensifer sojae CCBAU 05684 T in multilocus sequence analysis of recA, atpD and glnII. The genome average nucleotide identity values between representative strain YIC4027 T and the type strains of its closely related species were 81.6 to 88.9 %. Identical symbiotic gene (nodA, nodC and nifH) sequences highly similar with those in other Sesbania-nodulating strains (Rhizobium sp. SIN-1, Neorhizobium huautlense S02 T , Ensifer saheli ORS609 T and Rhizobium sp. IRBG74) were detected. The representative strain YIC4027 T could form effective nodules on its original host Sesbania cannabina, but not on Sophora flavescens, Trifolium repens, Glycine max, Glycyrrhiza uralensis, Phaseolus vulgaris or Medicago sativa. The use of lactulose as sole carbon source, possession of C 13 : 0 2-OH, C 13 : 1 at 12-13, C 15 : 1 iso !9c, C 17 : 0 anteiso and C 18 : 0 iso and absence of C 14 : 0 anteiso, C 15 : 0 anteiso and C 18 : 0 3-OH in fatty acids distinguished the strain YIC4027 T from the type strains of its closely related species. Based on all the analyses mentioned above, we propose a novel species Ensifer alkalisoli sp. nov. and designate YIC4027 T (=HAMBI 3655 T =LMG 29286 T ) as the type strain. The genome size of YIC4027 T is 5.97 Mbp, comprising 5588 predicted genes, and the DNA G+C content is 62.2 mol%.

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“…91 % 16S rRNA gene sequence similarity), which are rod-shaped and produce gelatinase (Shieh et al, 2004;Fukunaga et al, 2006). The nearest relatives within the order Rhizobiales, Mesorhizobium chacoense PR5 T (90 % similarity), Ensifer terangae LMG 7834 T (89.7 % similarity) and Pseudaminobacter salicylatoxidans BN12 T (89.5 % similarity), exhibit DNA G+C contents of 62, 61.6 and 63.9 mol%, respectively (Velázquez et al, 2001;Young, 2003;de Lajudie et al, 1994;Kämpfer et al, 1999). To date, only one representative of the 'Parvularculales' has been described (Cho & Giovannoni, 2003).…”

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confidence: 99%

Kiloniella laminariae gen. nov., sp. nov., an alphaproteobacterium from the marine macroalga Laminaria saccharina

Wiese

Thiel

Gärtner

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel alphaproteobacterium, strain LD81 T , was isolated from the marine macroalga Laminaria saccharina. The bacterium is mesophilic and shows a typical marine growth response. It is a chemoheterotrophic aerobe with the potential for denitrification. Growth optima are 25 6C, pH 5.5 and 3 % NaCl. Strain LD81 T has a unique phylogenetic position, not fitting any of the known families of the Alphaproteobacteria. The 16S rRNA gene sequence revealed a distant relationship to species of several orders of the Alphaproteobacteria, with less than 90 % sequence similarity. Phylogenetically, strain LD81 T is related to the type strains of Terasakiella pusilla (88.4 % 16S rRNA gene sequence similarity) and the three Thalassospira species (88.9-89.2 %). It forms a cluster with these bacteria and a novel as-yet undescribed isolate (KOPRI 13522; 96.6 % sequence similarity). Strain LD81 T has a relatively low DNA G+C content (51.1 mol%) and, due to its distant phylogenetic position from all other alphaproteobacteria, strain LD81 T (5NCIMB 14374 T 5JCM 14845 T ) is considered as the type strain of a novel species within a new genus, for which the name Kiloniella laminariae gen. nov., sp. nov. is proposed. The genus Kiloniella represents the type of the new family Kiloniellaceae fam. nov. and order Kiloniellales ord. nov.The Alphaproteobacteria is one of the most well-represented bacterial groups observed in marine habitats (Giovannoni & Rappé, 2000), with members of the orders Caulobacterales, Sphingomonadales, Rhizobiales, Rickettsiales, Rhodobacterales, Rhodospirillales, Kordiimonadales and 'Parvularculales' being reported (Garrity et al., 2005;Kwon et al., 2005). In a study concerning the phylogenetic analysis of bacteria that are associated with the marine brown alga Laminaria saccharina from the Baltic Sea, strain LD81 T was isolated.Pieces of Laminaria saccharina tissue were suspended in sterile seawater and homogenized using an Ultraturrax T25 (IKA Werke). The suspension was diluted in sterile seawater and plated on TSB medium (l 21 : 3 g Difco tryptic soy broth, 7 g NaCl, 15 g Bacto agar; pH 7.2). The plates were incubated at 22 u C in the dark for 20 days. After good growth was obtained, an overlay containing TSB medium (with 8 g l 21 Bacto agar) and 10 % (v/v) overnight culture of Candida glabrata DSM 6425 was poured onto the plates and incubated for 24 h at 22 u C in order to detect inhibition zones against C. glabrata by individual colonies. Antibiotically active colonies were repeatedly streaked on agar plates with TSB medium to obtain pure cultures. One of the pure cultures obtained was strain LD81 T , which was stored at 280 u C using the Cryobank System (Mast Diagnostica GmbH) for maintenance.Cell morphology was examined by scanning electron microscopy. Strain LD81 T was cultivated for 24 h in marine broth (MB; Difco 2216) at 28 u C on a rotary shaker with shaking at 95 r.p.m., followed by fixation with a final concentration of 1 % formol and filtration through 0.2 mm polycarbonate filters (Sarstedt). The filte...

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The genus name Ensifer Casida 1982 takes priority over Sinorhizobium Chen et al. 1988, and Sinorhizobium morelense Wang et al. 2002 is a later synonym of Ensifer adhaerens Casida 1982. Is the combination 'Sinorhizobium adhaerens' (Casida 1982) Willems et al. 2003 legitimate? Request for an Opinion

Cited by 174 publications

References 17 publications

Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

Ensifer alkalisoli sp. nov. isolated from root nodules of Sesbania cannabina grown in saline–alkaline soils

Kiloniella laminariae gen. nov., sp. nov., an alphaproteobacterium from the marine macroalga Laminaria saccharina

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