The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg

Chen, Da‐Yuan; Sun, Qing‐Yuan; Liu, Ji‐Long; Li, Guangpeng; Li, Lian; Wang, Minkang; Han, Zhiming; Song, Xie‐Yan; Li, Jinsong; Sun, Qiang; Chen, Yucun; Zhang, Yaping; Ding, Bo

doi:10.1007/bf02882053

Cited by 39 publications

(34 citation statements)

References 12 publications

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“…Additionally, a pregnancy was observed after the transfer of embryos reconstructed from cells of argali Ovis ammon and enucleated oocytes of domestic sheep [71]. In the giant panda, blastocysts have also been obtained after nuclear transfer of panda cells into rabbit enucleated oocytes [12]. Furthermore, it has clearly been demonstrated that bovine oocyte cytoplasm could also serve as recipient for somatic cells from different mammalian species [16].…”

Section: Other Biotechnologies and Future Applicationsmentioning

confidence: 99%

Reproductive biotechnologies for endangered mammalian species

2000

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-Assisted reproductive techniques (gamete cryopreservation, artificial insemination, embryo transfer, and in vitro fertilization) allow to propagate small fragmented populations of wild endangered species or domestic breeds. There are the best way for producing several offspring from selected genitors in order to avoid inbreeding depression. However, few mammalian species have been well studied for their reproductive biology whereas huge differences have been observed between these species. Furthermore, materials, methods and experimental designs have to be adapted for each case and each limiting factor (wildness, poor quantity of biological material, disparate locations). Genome resource banking is currently arising and the most applied reproductive biotechnology remains artificial insemination. Assisted reproductive techniques currently developed in domestic species (intracytoplasmic sperm injection, nuclear transfer) may offer new opportunities for the propagation of endangered species. endangered species / artificial insemination / cryopreservation / in vitro fertilization / embryo Résumé -Biotechnologies de la reproduction appliquées aux mammifères en voie de disparition. Les techniques de reproduction assistée (cryoconservation des gamètes, insémination artificielle, transfert embryonnaire, fécondation in vitro) permettent d'accroître des populations, parfois dispersées géographiquement, d'espèces sauvages ou domestiques en voie de disparition. Ces méthodes sont le meilleur moyen pour produire plusieurs descendants à partir de géniteurs sélectionnés de façon à éviter la consanguinité. La biologie de la reproduction est connue pour peu d'espèces de mammifères alors que de grandes différences ont pourtant été mises en évidence entre ces espèces. En outre, les matériels, les méthodes, ainsi que les schémas expérimentaux doivent être adaptés à chaque cas et pour chaque facteur limitant (animaux sauvages, peu de matériel biologique, populations dispersées). Des banques génétiques sont actuellement en voie de constitution mais la technique la plus utilisée reste l'insémination artificielle. Cependant, des biotechnologies de la reproduction en cours de mise au point chez les animaux domestiques (injection intra-cytoplasmique de spermatozoïde, transfert nucléaire) pourraient offrir de nouvelles possibilités pour l'accroissement de populations menacées. espèces menacées / insémination artificielle / congélation / fécondation in vitro / embryon Reprod. Nutr. Dev. 40 (2000)

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Section: Other Biotechnologies and Future Applicationsmentioning

confidence: 99%

Reproductive biotechnologies for endangered mammalian species

2000

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“…gram Panda somatic nuclei and have supported development of nt-units to the blastocyst stage [12]. In this study, we tested rabbit oocyte cytoplasm as a means to reprogram human somatic cell nuclei, and demonstrate that the derived cells possess the properties and phenotypes of conventional human ES cells, that they retain a normal karyotype, and that they are capable of multilineage cellular differentiation.…”

Section: Introductionmentioning

confidence: 95%

“…For example, when cultures were switched, after retinoic acid induction, to a neuron-inducing medium composed of 6, 11, 14), human blood cells (lanes 2, 7), rabbit blood cells (lanes 4, 9, 15) or mouse feeders (lanes 3,8,12) were amplified by primer sets specific for human chromosome 7 alphoid repeats (lanes 1, 2, 3 The differentiated ntES cells derived from these fibroblasts. DNA samples were analyzed by multiplex PCR amplification of 14 microsatellite markers: 1, D3s1358; 2, vWA; 3, FGA; 4, Amelogenin; 5, D8s1179; 6, D21s11; 7, D18s51; 8, D5s818; 9, D13s317; 10, D7s820; 11, D16s539; 12, TH01; 13, TPOX; 14, CSF1PO.…”

Section: Somatic Differentiation Of Ntes Cellsmentioning

confidence: 99%

Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

Chen¹,

Liu

et al. 2003

Cell Res

200

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To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups. These results suggest that human somatic nuclei can form ntES cells independent of the age of the donor. The derived ntES cells are human based on karyotype, isogenicity, in situ hybridization, PCR and immunocytochemistry with probes that distinguish between the various species. The ntES cells maintain the capability of sustained growth in an undifferentiated state, and form embryoid bodies, which, on further induction, give rise to cell types such as neuron and muscle, as well as mixed cell populations that express markers representative of all three germ layers. Thus, ntES cells derived from human somatic cells by NT to rabbit eggs retain phenotypes similar to those of conventional human ES cells, including the ability to undergo multilineage cellular differentiation.

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“…Even interspecies nuclear transfer has resulted offspring from a gaur (Lanza et al, 2002). Cows, rabbits, and sheep oocytes can support early development of embryos produced by nuclear transfer of somatic cell nuclei from other species (Chen et al, 1999;Dominko et al, 1999;White et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Cell cycle analysis and interspecies nuclear transfer of in vitro cultured skin fibroblasts of the Siberian tiger (Panthera tigris Altaica)

Hashem

Bhandari

Kang

et al. 2006

Molecular Reproduction Devel

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The present study was conducted to examine the effect of cell culture conditions, antioxidants, protease inhibitors (PI), and different levels of dimethylsulfoxide (DMSO) for the promotion of synchronization of different cell cycles of Siberian tiger skin fibroblasts. We also compared the ability of somatic cell nuclei of the Siberian tiger in pig cytoplasts and to support early development after reconstruction. Cell cycle synchronization between nuclear donor and recipient cells is considered to be one of the most crucial factors for successful cloning. Five experiments were performed each with a one-way completely randomized design involving three replicates of all treatments. Least significant difference (LSD) was used to determine variation among treatment groups. Experiment I focused in the effects of cycling, serum starved and fully confluent stages of Siberian tiger cells on different cell cycles. In Experiment II, the effects of different antioxidants like b-Mercaptoethanol (b-ME, 10 mM), cysteine (2 mM), and glutathione (2 mM) were examined after cells were fully confluent without serum starvation for 4 hr. In Experiment III, three PI, namely 6-dimethylaminopurine (6-DMAP, 2 mM), cycloheximide (7.5 mg/ml) and cytochalasin B (7.5 mg/ ml) were used in the sane manner as in Experiment II. In Experiment IV, different levels of DMSO at 0%, 0.5%, 1.0%, and 2.5% were tested on different cell cycle stages of Siberian tiger examined by Flowcytometry (FACS). In Experiment I, 67.2% of the Siberian tiger skin fibroblasts reached the G 0 /G 1 stage (2C DNA content) in fully confluent conditions which was more than the cycling (49.8%) and serum starved (SS) medium (65.5%; P < 0.05). Among the chemically treated group, glutathione (72.6%) and cycloheximide (71.3%) had little bit better results for the synchronization of G 0 þ G 1 phases than serum starved and fully confluent. After nuclear transfer we did not see any significant differences on the development of tigerporcine reconstructed embryos at cycling, SS and fully confluent. Data indicate that prolonged culture of cells in the absence of serum as well as using different chemicals for this experiment does not imply a shift in the percentage of cells that enter G 0 /G 1 and that confluency is sufficient to induce quiescence. This finding can be beneficial in nuclear transfer programs in Siberian tiger, because there are negative effects, such as apoptosis associated with serum starvation. Mol. Reprod. Dev. 74: 403-411, 2007. ß

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The giant panda (Ailuropoda melanoleuca) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg

Cited by 39 publications

References 12 publications

Reproductive biotechnologies for endangered mammalian species

Reproductive biotechnologies for endangered mammalian species

Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes

Cell cycle analysis and interspecies nuclear transfer of in vitro cultured skin fibroblasts of the Siberian tiger (Panthera tigris Altaica)

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