The gingival immune response to periodontal pathogens in juvenile periodontitis

Hall, Eric R.; Martin, Stephen A.; Suzuki, Jon B.; Falkler, William A.

doi:10.1111/j.1399-302x.1994.tb00282.x

Cited by 12 publications

(8 citation statements)

References 39 publications

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“…The tenuous nature of the link between P. anaerobius and periodontal disease is further documented by the immunological findings of another study. With a gingival explant culture system, Hall et al [21] evaluated the reactivity of local antibodies produced by juvenile periodontitis tissue. Only 2 of 73 gingival explant culture supernatant fluids demonstrated reactivity to P. anaerobius, indicating that this bacterium does not play a major role in periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

Development of a PCR assay specific for Peptostreptococcus anaerobius

Riggio

Lennon

2002

Journal of Medical Microbiology

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Peptostreptococcus anaerobius is a gram-positive anaerobic coccus that is widely distributed in the normal human flora. The organism has also been implicated as a causative agent of several systemic infections, including endocarditis and infections of the genitourinary and gastrointestinal tracts. Its role in oral disease is less well defined, although it has been implicated in periodontal disease, gingivitis and root canal infections. Identification of P. anaerobius in clinical samples is currently reliant upon traditional culture and biochemical methods. The aim of this study was to develop a novel PCR assay for the detection of P. anaerobius and to attempt detection of this organism in oral samples. PCR primers specific for P. anaerobius DNA were developed by alignment of bacterial 16S ribosomal RNA gene sequences and selection of sequences specific at their 39 ends for P. anaerobius. When used in a PCR assay, positivity for P. anaerobius DNA was indicated by the amplification of a 943-bp product. The primers were shown to be specific for P. anaerobius DNA, as no PCR products were obtained when genomic DNA from a wide range of other Peptostreptococcus species and other oral bacteria were used as templates. The PCR assay was then applied to the detection of P. anaerobius DNA in subgingival plaque samples from adult periodontitis patients and pus aspirates from subjects with acute dento-alveolar abscesses. All of 60 subgingival plaque samples from 16 patients were negative for P. anaerobius DNA. None of the 43 pus samples analysed contained P. anaerobius DNA. These results suggest that P. anaerobius is not a major pathogen in adult periodontitis and dento-alveolar abscesses. The PCR assay is a more rapid, sensitive and specific alternative to culturebased methods for identification of P. anaerobius in clinical samples.

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Section: Discussionmentioning

confidence: 99%

Development of a PCR assay specific for Peptostreptococcus anaerobius

Riggio

Lennon

2002

Journal of Medical Microbiology

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“…An even higher prevalence of P. micros in the subgingival plaque of adult periodontitis patients has been reported, with 112 (91%) of 123 patients shown to harbour the organism as demonstrated by culture methods [19]. The gingival immune response to periodontal pathogens in juvenile periodontitis patients has been examined by a gingival explant culture system, with local IgG levels to P. micros shown to be elevated in these patients compared with healthy controls [20].…”

Section: Discussionmentioning

confidence: 99%

Detection of Peptostreptococcus micros DNA in clinical samples by PCR

Riggio

Lennon

Smith

2001

Journal of Medical Microbiology

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Peptostreptococcus micros is a gram-positive anaerobic coccus which, although considered to be a natural commensal of the human oral cavity, is associated with periodontal, endodontal and peritonsillar infections. Identi®cation of the organism has to date relied upon conventional culture methods and biochemical analyses. The purpose of this study was to develop a PCR method for rapid and speci®c identi®cation of this organism in clinical samples. A pair of primers was selected, each of which was speci®c at the 39 end for P. micros DNA; they were used in the PCR assay, resulting in a 1074-bp product. The primers were shown to be speci®c for P. micros DNA as no PCR products were obtained when genomic DNA extracts from a wide range of other Peptostreptococcus species and other oral bacteria were used as templates. The PCR assay was then applied to the identi®cation of P. micros DNA in subgingival plaque samples from adult periodontitis patients and pus samples from subjects with acute dento-alveolar abscesses. Con®rmation of speci®c ampli®cation of P. micros DNA was obtained by digestion of PCR products with the restriction endonuclease RsaI, which gives a unique restriction pro®le for P. micros, and DNA sequencing. Sixty-eight subgingival plaque samples from 18 patients were analysed, of which 19 (28%) were positive for P. micros DNA; the proportion of patients carrying P. micros DNA in at least one sampled site was 11 (61%) of 18. Twenty (71%) of 28 pus samples analysed by PCR contained P. micros DNA. These results con®rm that P. micros may be involved in the aetiology of acute dentoalveolar abscesses and adult periodontitis. The PCR assay provides a more rapid and reliable alternative to conventional methods for identi®cation of P. micros in clinical samples.

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“…On the other hand, the induction of suppressive monocytes by F. nucleatum may result in the inhibition of host-protective immune reactions (195). Local suppression of specific antibody production by F. nucleatum may be the reason why Hall et al (116) found immunoglobulins to this bacterium in supernatant fluid from juvenile periodontitis tissues in only 1 of 75 patients, even though this microorganism is often isolated from subgingival plaque of such patients and F. nucleatum-specific antibodies have been detected in their sera (203,314). As discussed by Shenker (257), immunosuppression must be a relatively tem-porary phenomenon since many patients eventually develop a detectable humoral and/or cellular immune response to periodontal infection.…”

Section: Immunological Aspectsmentioning

confidence: 99%