The GIP gamma-tubulin complex-associated proteins are involved in nuclear architecture in Arabidopsis thaliana

Batzenschlager, Morgane; Masoud, Kinda; Janski, Natacha; Houlné, Guy; Herzog, Étienne; Evrard, Jean-Luc; Baumberger, Nicolas; Erhardt, Mathieu; Nominé, Yves; Kieffer, Bruno; Schmit, Anne‐Catherine; Chabouté, Marie‐Edith

doi:10.3389/fpls.2013.00480

Cited by 52 publications

(107 citation statements)

References 69 publications

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“…26 Interestingly, the minimal domain of interaction of TSK was partly overlapping with that of GIP1 in the C-terminal region of TSA1. 6,9 These data argue for a spatiotemporal regulation of GIP1, TSA1 and MGO3 interactions to properly ensure centromere cohesion. In addition, in tsk mutants, a stabilization of TSA1 was observed without any changes in the TSA1 transcript level, 16 contrary to mgo3 in which we showed that TSA1 was upregulated (3 to 4 times, Fig.…”

Section: Discussionmentioning

confidence: 99%

“…4 Recently, we demonstrated that GIPs, initially found as regulators of the recruitment of microtubule-nucleation complexes, were also key players in the regulation of the nuclear architecture. 5,6 GIPs located on both sides of the Nuclear Envelope (NE) play a critical role in the maintenance of centromeric cohesion in the nuclei of cycling cells studied in Arabidopsis root meristems. 7,8 Interestingly, GIPs were identified as partners of TSA1 6 which was first described as TONSOKU (TSK)-associating protein1.…”

Section: Introductionmentioning

confidence: 99%

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MGO3 and GIP1 act synergistically for the maintenance of centromeric cohesion

Batzenschlager

Schmit

Herzog

et al. 2016

Nucleus

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The control of genomic maintenance during S phase is crucial in eukaryotes. It involves the establishment of sister chromatid cohesion, ensuring faithful chromosome segregation, as well as proper DNA replication and repair to preserve genetic information. In animals, nuclear periphery proteins -including inner nuclear membrane proteins and nuclear pore-associated componentsare key factors which regulate DNA integrity. Corresponding functional homologues are not so well known in plants which may have developed specific mechanisms due to their sessile life. We have already characterized the Gamma-tubulin Complex Protein 3-interacting proteins (GIPs) as essential regulators of centromeric cohesion at the nuclear periphery. GIPs were also shown to interact with TSA1, first described as a partner of the epigenetic regulator MGOUN3 (MGO3)/BRUSHY1 (BRU1)/ TONSOKU (TSK) involved in genomic maintenance. Here, using genetic analyses, we show that the mgo3gip1 mutants display an impaired and pleiotropic development including fasciation. We also provide evidence for the contribution of both MGO3 and GIP1 to the regulation of centromeric cohesion in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MGO3 and GIP1 act synergistically for the maintenance of centromeric cohesion

Batzenschlager

Schmit

Herzog

et al. 2016

Nucleus

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“…However, it is known that γ-tubulin complex (γ-TuC) components are associated with the nuclear envelope and that microtubule growth can initiate on the nuclear surface (Batzenschlager et al, 2013). Proteins involved are γ-TuC protein 3 (GCP3) and GCP3-interacting protein (GIP) (Janski et al, 2012;Batzenschlager et al, 2015).…”

Section: Mitosis and Meiosismentioning

confidence: 99%

“…Proteins involved are γ-TuC protein 3 (GCP3) and GCP3-interacting protein (GIP) (Janski et al, 2012;Batzenschlager et al, 2015). GCP3 interacts with GIP, and GIP in turn interacts with TonSoku-associating protein 1 (TSA1), a TMD protein associated with the nuclear envelope (Batzenschlager et al, 2013). It could therefore be tested whether members of the WIP and/ or WIT families interact with any components of the plant γ-TuC that are associated with the nuclear envelope, such as GIP or GCP3 (Fig.…”

Section: Mitosis and Meiosismentioning

confidence: 99%

LINCing the eukaryotic tree of life – towards a broad evolutionary comparison of nucleocytoplasmic bridging complexes

Meier

2016

Journal of Cell Science

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The nuclear envelope is much more than a simple barrier between nucleoplasm and cytoplasm. Nuclear envelope bridging complexes are protein complexes spanning both the inner and outer nuclear envelope membranes, thus directly connecting the cytoplasm with the nucleoplasm. In metazoans, they are involved in connecting the cytoskeleton with the nucleoskeleton, and act as anchoring platforms at the nuclear envelope for the positioning and moving of both nuclei and chromosomes. Recently, nucleocytoplasmic bridging complexes have also been identified in more evolutionarily diverse organisms, including land plants. Here, I discuss similarities and differences among and between eukaryotic supergroups, specifically of the proteins forming the cytoplasmic surface of these complexes. I am proposing a structure and function for a hypothetical ancestral nucleocytoplasmic bridging complex in the last eukaryotic common ancestor, with the goal to stimulate research in more diverse emerging model organisms.

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“…They also point to divergences with ARK1 having a broad function during the entire root hair developmental program and AGD1 being more specifically involved in the early stages of root hair initiation and tip growth. Batzenschlager et al (2013) bring us into a functional study suggesting that the Arabidopsis γ-tubulin complex (γ-TuC) components GCP3-interacting protein1 (GIP1) and GIP2 are key determinants of nuclear shaping and nuclear envelope organization. The authors also report the identification of a novel and direct partner of AtGIP1, namely AtTSA1, which partially colocalizes with AtGIP1 at the nuclear envelope.…”

Section: Trafficking On the Cytoskeleton Toward The Plasma Membrane Amentioning

confidence: 99%

A dynamic interplay between membranes and the cytoskeleton critical for cell development and signaling

2014

Frontiers Research Topics

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Diverse cellular processes that underlie plant development and response to environmental cues rely on a dynamic interplay between membranes and the cytoskeleton. The original research and review articles assembled in this Research Topic provide a valuable insight into our current understanding of how actin filaments and microtubules physically and functionally interact with membranes and cell wall and vice versa. ACTIN-BINDING PROTEINS: OLD AND NEWActin-binding proteins come in hundreds of different varieties and are the governors for cellular cytoskeletal organization and turnover. The rate limiting step for generating actin filaments from a monomer pool in vitro, and presumably in vivo, is formation of a seed or nucleus of three actin subunits. Formins are conserved actin nucleation factors which, in plants, take on special relevance for the presence of secretion signals, transmembrane domains and lipid interaction motifs that permit their function on or near cellular membranes. In an up-to-date review, Cvrčková (2013) discusses the myriad ways that plant formins are thought to associate with membranes and their function(s) at various subcellular locations, especially the interface between cortical actin cytoskeleton-plasma membrane-cell wall.The monomer-binding protein, profilin, was the first conventional plant actin-binding protein to be identified more than two decades ago. Sun et al. (2013) briefly review the long history of discovery research surrounding this abundant regulator of actin dynamcis. Biochemically, isoforms from maize and Arabidopsis have been well characterized and these bind three cellular ligands: monomer of G-actin, proteins with contiguous stretches of proline residues, and phosphoinositide lipids. More recently, it has been discovered that plant profilins are localized or function at cellular membranes and organelles via their interaction with nucleators like the formins; here, profilinactin complexes likely provide a supply of subunits to polymerize new actin filaments. Whether this actin assembly powers vesicle or organelle movements, as in the case of mammalian endosomes or pathogenic intracellular bacteria, is an area for future investigations.One striking difference between the repertoire of actinbinding proteins from plants and animals is that many types of actin-membrane adaptors present in animals are absent from plants. Plants in turn own a plant-specific superfamily of actin-binding proteins, called the Networked (NET) proteins; presumably these are able to connect actin filaments to various membrane compartments. Here, Hawkins et al. (2014) report a phylogenetic analysis including NET gene sequences across a large range of species. Their data support that the NET superfamily emerged early in the evolution of vascular plants and has continuously developed and diversified coincidentally with the complexity of plant species. CORTICAL MICROTUBULES AND CELL WALL ASSEMBLYA remarkable parallelism between membrane-associated microtubules in the cortical cytoplasm and cellulose micro...

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The GIP gamma-tubulin complex-associated proteins are involved in nuclear architecture in Arabidopsis thaliana

Cited by 52 publications

References 69 publications

MGO3 and GIP1 act synergistically for the maintenance of centromeric cohesion

MGO3 and GIP1 act synergistically for the maintenance of centromeric cohesion

LINCing the eukaryotic tree of life – towards a broad evolutionary comparison of nucleocytoplasmic bridging complexes

A dynamic interplay between membranes and the cytoskeleton critical for cell development and signaling

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