This inquiry-based activity introduces
students to the CRISPR method
of genetic engineering and recent applications of CRISPR-editing.
This activity was implemented in a nonmajors chemistry course for
first-year students, during a 1 h 50 min laboratory period. However,
the activity could easily be adapted, and it is useful for meeting
scientific literacy learning goals of a college chemistry course for
majors, an introductory college biochemistry course, or an advanced
high school chemistry or biology course. For the activity, students
self-selected into small groups and then researched a particular topic
concerning CRISPR using a series of questions designed to guide their
discovery work. Subtopics included both biochemical and application
details of CRISPR, as well as social and ethical considerations concerning
the genetic engineering of humans. The activity was designed to introduce
students to this rapidly burgeoning biochemical method using a systems-based
analysis in which social and ethical considerations are considered
along with technical details. Groups prepared a short written and
oral summary of their findings, and the instructor facilitated a class
discussion on the topic following group oral presentations. Pre- and
postactivity survey analysis indicated that students found the activity
worthwhile and were able to reflect on the potential broader social
and ethical implications of CRISPR-editing due to the research and
discussions facilitated by the activity.