2023
DOI: 10.1128/spectrum.03820-22
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The global transcriptome of Plasmodium falciparum mid-stage gametocytes (stages II–IV) appears largely conserved and gametocyte-specific gene expression patterns vary in clinical isolates

Jonas A. Kengne-Ouafo,
Saikou Y. Bah,
Alison Kemp
et al.

Abstract: Our overall understanding of the developmental biology of malaria parasites has been greatly enhanced by recent advances in transcriptomic analysis. However, most of these investigations rely on laboratory strains (LS) that were adapted into in vitro culture many years ago, and the transcriptomes of clinical isolates (CI) circulating in human populations have not been assessed. In this study, RNA-seq was used to compare the global transcriptome of mid-stage gametocytes derived from thre… Show more

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Cited by 5 publications
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“…Although the major limitation of nanopore sequencing is its lower read accuracy when compared with shortread technologies, the advantages of long reads outweigh low reads' accuracy [44]. ONT long-read sequencing provides real-time, amplification-free, single-molecule sequencing of cDNAs that enables the recovery of full-length transcripts without the need for an assembly step, and so, quantifying the expression of genes can be achieved via simple counting of the assigned reads [34,45,46].…”
Section: Discussionmentioning
confidence: 99%
“…Although the major limitation of nanopore sequencing is its lower read accuracy when compared with shortread technologies, the advantages of long reads outweigh low reads' accuracy [44]. ONT long-read sequencing provides real-time, amplification-free, single-molecule sequencing of cDNAs that enables the recovery of full-length transcripts without the need for an assembly step, and so, quantifying the expression of genes can be achieved via simple counting of the assigned reads [34,45,46].…”
Section: Discussionmentioning
confidence: 99%