The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system

Shu, Shijie; Ju, Gong; Fan, Lingzhi

doi:10.1016/0304-3940(88)90346-1

Cited by 1,174 publications

(595 citation statements)

References 8 publications

Supporting

Mentioning

593

Contrasting

Unclassified

Order By: Relevance

“…Control sections were incubated only in PB without the lectin. After three washes in PBS, all sections were incubated with the avidin-biotin-peroxidase complex (ABC, 1:200, Vector, USA) and peroxidase was revealed using the diaminobenzidine reaction intensified with nickel ammonium sulfate [58]. Finally, sections were dehydrated and cover slipped with Entellan (Merck, Germany).…”

Section: Histological Analysismentioning

confidence: 99%

Effect of chronic stress during adolescence in prefrontal cortex structure and function

Folha

Bahia

Aguiar

et al. 2017

Behavioural Brain Research

View full text Add to dashboard Cite

h i g h l i g h t s• Chronic stress impairs the development of the adolescent brain.• Chronic stress delays the maturation of extracellular structures called perineuronal nets, which stabilize synaptic contacts on inhibitory neurons, and prefrontal cortex-associated behavior.• These negative effects of chronic stress on the prefrontal cortex can theoretically occur in humans. Critical periods of plasticity (CPPs) are defined by developmental intervals wherein neuronal circuits are most susceptible to environmental influences. The CPP of the prefrontal cortex (PFC), which controls executive functions, extends up to early adulthood and, like other cortical areas, reflects the maturation of perineuronal nets (PNNs) surrounding the cell bodies of specialized inhibitory interneurons. The aim of the present work was to evaluate the effect of chronic stress on both structure and function of the adolescent's rat PFC. We subjected P28 rats to stressful situations for 7, 15 and 35 days and evaluated the spatial distribution of histochemically-labeled PNNs in both the Medial Prefrontal Cortex (MPFC) and the Orbitofrontal Cortex (OFC) and PFC-associated behavior as well. Chronic stress affects PFC development, slowing PNN maturation in both the (MPFC) and (OFC) while negatively affecting functions associated with these areas. We speculate upon the risks of prolonged exposure to stressful environments in human adolescents and the possibility of stunted development of executive functions.

show abstract

Section: Histological Analysismentioning

confidence: 99%

Effect of chronic stress during adolescence in prefrontal cortex structure and function

Folha

Bahia

Aguiar

et al. 2017

Behavioural Brain Research

View full text Add to dashboard Cite

show abstract

“…The reaction was terminated by incubating with 2 Â SSC for 15 min at room temperature and the product visualized with the glucose-oxidase-DAB-nickel method, 22 and counterstained with Eosin. Omission of the terminal transferase served as a negative control.…”

Section: Histologymentioning

confidence: 99%

Secondary degeneration reduced by inosine after spinal cord injury in rats

et al. 2005

Spinal Cord

View full text Add to dashboard Cite

Study design: Assessment of the potential protective effects of inosine on an animal model of spinal cord injury. Objectives: Our previous studies have demonstrated that inosine can directly protect neurons in vitro from zinc-induced injury and axotomized retinal ganglion cells of rats in vivo. This investigation was carried out to examine the possible protective effects of inosine on spinal cord secondary degeneration. Setting: Institute of Neurosciences, The Fourth Military Medical University, Xi'an, China. Methods: Compressive spinal cord injury (95-g load for 1 min) model was established in rats, and inosine was administrated beginning at different time points (2, 12, or 24 h) after spinal cord injury. Results: Using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) technique and hematoxylin and eosin staining, our study demonstrated that administration of inosine as late as 12 h after injury significantly reduced the total volume of spinal cord degenerative areas and the number of apoptotic cells 3 days following the trauma. Conclusion: Inosine can significantly reduce the spread of secondary degeneration and the cell death following spinal cord injury in adult rats. These findings may find a clinical application in the treatment of acute spinal cord injury.

show abstract

“…k159, Santa Cruz Biotechnology, Santa Cruz, Calif., USA) in PBS containing 0.02% Triton-X-100, 1% gelatin, and 5% foetal bovine serum. After three rinses in PBS, sections were processed according to the immunoperoxidase procedure (ABC kit, elite, Vector Burlingame, Calif., USA) using 3-3′di-aminobenzidine as chromogen combined with nickel enhancement [19]. After several rinses in PBS, sections were mounted in slide glasses, dried, dehydrated gradually in ethanol, cleared in xylene and covered with DEPEX (Serva, Heidelberg, Germany).…”

Section: Immunohistochemical Detection Of Leptin Receptorsmentioning

confidence: 99%

Long-term food restriction prevents ageing-associated central leptin resistance in wistar rats

et al. 2002

View full text Add to dashboard Cite

Aims/hypothesis. Ageing is associated with insulin and leptin resistance in mammals. These alterations might be caused by the increased adiposity associated with ageing, by ageing alone or both. We studied whether leptin resistance occurs at the central level in the Wistar rat and we aimed to discriminate between the effects of ageing from those of the increased adiposity associated with ageing. Methods. Leptin was infused intracerebroventricularly at a constant rate in young adult, old and old Wistar rats fasted for 3 months, using osmotic pumps. The effects on body weight, daily food intake, Lee index, adiposity and serum leptin values were analysed. The effect of food restriction on the expression of the long form of leptin receptor in the hypothalamus was also studied. Results. Leptin decreased daily food intake and body weight in young and old Wistar rats. With a dose of 10 µg/day similar responses were obtained in young and old rats but with a dose of 0.2 µg/day, only young rats showed decreases in these parameters. Foodrestriction in old rats lowered adiposity and serum leptin to values close to those of young rats, recovered responsiveness to i.c.v. administration of leptin at the dose of 0.2 µg/day and increased leptin receptor expression in the hypothalamus. Conclusion/interpretation. Our data show that old Wistar rats have a decreased response to leptin at the central level. Food-restriction recovers leptin responsiveness and increases leptin receptor in the hypothalamus suggesting that adiposity plays a key role in the development of leptin resistance associated with ageing. [Diabetologia (2002[Diabetologia ( ) 45:997-1003 Keywords Leptin, leptin-resistance, food-restriction, ageing, Wistar rat.

show abstract

The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system

Cited by 1,174 publications

References 8 publications

Effect of chronic stress during adolescence in prefrontal cortex structure and function

Effect of chronic stress during adolescence in prefrontal cortex structure and function

Secondary degeneration reduced by inosine after spinal cord injury in rats

Long-term food restriction prevents ageing-associated central leptin resistance in wistar rats

Contact Info

Product

Resources

About