The Glutathione S-Transferase PtGSTF1 Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar

Abstract

Glutathione S-transferases (GSTs) play an essential role in plant cell detoxification and secondary metabolism. However, their accurate functions in the growth and response to abiotic stress in woody plants are still largely unknown. In this work, a Phi class Glutathione S-transferase encoding gene PtGSTF1 was isolated from poplar (P. trichocarpa), and its biological functions in the regulation of biomass production and salt tolerance were investigated in transgenic poplar. PtGSTF1 was ubiquitously expressed in various tissues and organs, with a predominant expression in leaves and inducible expression by salt stress. Transgenic poplar overexpressing PtGSTF1 showed improved shoot growth, wood formation and improved salt tolerance, consistent with the increased xylem cell number and size under normal condition, and the optimized Na+ and K+ homeostasis and strengthened reactive oxygen species scavenging during salt stress. Further transcriptome analyses demonstrated that the expressions of genes related to hydrolase, cell wall modification, ion homeostasis and ROS scavenging were up- or down-regulated in transgenic plants. Our findings imply that PtGSTF1 improves both biomass production and salt tolerance through regulating hydrolase activity, cell wall modification, ion homeostasis and ROS scavenging in transgenic poplar, and that it can be considered as a useful gene candidate for the genetic breeding of new tree varieties with improved growth under salt stress conditions.

RVE1, DBB1b, and COL2 Transcription Factors Are Responsive to Combined Stress by UV-B Radiation and Cold in Bell Pepper (Capsicum annuum)

“…Moreover, in transgenic plants overexpressing PtGSTF1, malondialdehyde accumulation was significantly lower, and GST, SOD, and CAT activity were higher than in wild-type plants. [76]. These results suggest that RVE1 may have a principal role in regulating the enzymatic antioxidant defense in coping with UV-B radiation and cold.…”

Weighted gene co-expression network analysis reveals hub genes regulating response to salt stress in peanut

“…Arahy.DF20BV encodes glutathione S-transferase, which plays a crucial role in plant cell detoxification and is closely associated with both biotic and abiotic stresses. Studies have shown that it can enhance salt tolerance in plants like Arabidopsis and Poplar [ 73 , 74 ]. Additionally, arahy.TN1PNA and arahy.5L9AX6 encode fatty acyl-CoA reductase 3 (FAR), which catalyzes the reduction of fatty acyl-CoA or fatty acyl carrier protein substrates, contributing to primary alcohol formation and participating in wax synthesis.…”

Comparative Analysis of the Effect of Gamma-, Electron, and Proton Irradiation on Transcriptomic Profile of Hordeum vulgare L. Seedlings: In Search for Molecular Contributors to Abiotic Stress Resilience

“…Plant glutathione-S-transferase proteins (GSTs) have been shown to regulate redox homeostasis by modulating glutathione content and redox state [61]. GSTs also participate in light signalling in Arabidopsis [62], in salt tolerance through regulating xylem cell proliferation, ion homeostasis, and reactive oxygen species scavenging in poplars [63], and in plant drought tolerance [64]. The glutathione-S-transferase T3-like protein contains the NAM-domain (No Apical Meristem) and showed discrete localisation within the nucleus in Arabidopsis, possibly serving a role in reducing nucleic acid hydroperoxides or in signalling [65].…”