The glutathione transferase family of Chlamydomonas reinhardtii: Identification and characterization of novel sigma class-like enzymes

Chatzikonstantinou, Marianna; Vlachakis, Dimitriοs; Chronopoulou, Evangelia; Papageorgiou, Louis; Papageorgiou, Anastassios C.; Labrou, Nikolaos E.

doi:10.1016/j.algal.2017.03.010

Cited by 18 publications

(10 citation statements)

References 52 publications

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“…GSTs quench reactive molecules with the conjugation of GSH and detoxify cells from oxidative damage [ 68 , 69 ]. GSTs are involved in the detoxification mechanisms, and 15 GST-like isoenzymes (Cr GSTS) were found in the model species of green alga Chlamydomonas reinhardtii [ 70 ].…”

Section: Resultsmentioning

confidence: 99%

“…GSTs quench reactive molecules with the conjugation of GSH and detoxify cells from oxidative damage [68,69]. GSTs are involved in the detoxification mechanisms, and 15 GST- Data are presented as means ± SD (n = 3) in each column, and for each concentration, means with different letters are significantly different (p < 0.05) like isoenzymes (Cr GSTS) were found in the model species of green alga Chlamydomonas reinhardtii [70]. Moreover, Zahang et al [71] found that sulfur significantly enhanced the tolerance of oil seed rape exposed to Cr stress by activating several detoxification mechanisms such as the ascorbate-glutathione (AsA-GSH) enzyme defense system and GSH production.…”

Section: Effect Of Culture Media Composition On Secondary Metabolite Production Of N Linckiamentioning

confidence: 99%

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Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

El-fayoumy

Shanab

Hassan

et al. 2021

Biomass Conv. Bioref.

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The current work aims to investigate the effect of abiotic stresses (nitrogen (N) and sulfur (S) [0.0 g/l, 1.5 g/l, 3 g/l, 6 g/l, and 12 g/l N and 0.0 g/l, 0.07 g/l, 0.15 g/l, 0.3 g/l, and 0.6 g/l S] and their combination [0.3 g/l S + 6 g/l N]) of axenic culture of Nostoc linckia on the production of secondary metabolites which induce different biological activities. Growth rate was measured by dry weight (DW) and optical density (OD) 550 nm . Additionally, phytochemical compounds, defense enzymes as well as antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(ethylbenzthiazoline-6-sulfonic acid) (ABTS ·+ ) radical assays of crude extracts (methylene chloride:methanol, 1:1) were evaluated. Based on antioxidant activity, four promising extracts were subjected to different biological activities such as anticancer, antimicrobial, and antiviral activities. The obtained results revealed that supplementation of external nitrogen source in the form of sodium nitrate was found to increase the total phycobiliprotein content by fivefold. Also, nitrogen depletion provoked significantly highest quantities of phenolic and flavonoid content and this has effects on biological activities of Nostoc linckia . Moreover, 0.3 g/l S was found to be the most effective extract exhibiting a significant increase in antioxidant activity based on DPPH and ABTS assays, respectively (88.18 ± 0.64% and 84.20 ± 1.01%). Furthermore, it recorded anticancer activity against HCT 116 cell line with IC 50 of 155 μg/ml. Moreover, this extract possessed a noticeable antibacterial potency (21.0 ± 1.0 as mm inhibition zone against Staphylococcus aureus and 19.3 ± 0.6 against Streptococcus mutans ). In addition, its antiviral activity against H 5 N 1 virus as a percentage of inhibition was 50% and 63.6% at a concentration of 7 μg/ml and 28 μg/ml, respectively, with cytotoxicity less than 7 μg/μl. GC-MS analysis recorded the presence of bioactive compounds exhibiting different biological activities. Therefore, the obtained results can represent valuable bioactive compounds with variable biological potencies.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Culture Media Composition On Secondary Metabolite Production Of N Linckiamentioning

confidence: 99%

Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

El-fayoumy

Shanab

Hassan

et al. 2021

Biomass Conv. Bioref.

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“…Glutathione‐ S ‐transferase is a phase II biotransformation enzyme that plays an important role in the detoxification of many xenobiotic compounds (Dos Santos et al, 2021), including atrazine (Cummins et al, 2011). Studies show that this enzyme is one of the main enzymes of the defense system of microalgae (Chatzikonstantinou et al, 2017). The microalgae P. boryanum also presented a smaller amount of Chl‐a.…”

Section: Discussionmentioning

confidence: 99%

Differential Effects of Atrazine on Chlorophyceae Species and Association with Morphology, Photosynthesis, Chlorophyll Content, and Glutathione‐S‐Transferase Activity

Castro

Silva

Machado

et al. 2022

Enviro Toxic and Chemistry

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Atrazine is a herbicide widely used in the control of weeds in crops such as corn, sugar cane, and sorghum. It is often found in aquatic environments, where it can potentially endanger nontarget organisms such as microalgae. The present study evaluated atrazine toxicity to seven different species of Chlorophyceae and the tolerance of the species to the herbicide was related to morphological, photosynthetic, chlorophyll‐a content and the activity of the glutathione‐S‐transferase enzyme (GST). The comparison of median effect concentration (EC50) values for growth inhibition indicates higher toxicity of atrazine for Pseudopediastrum boryanum and Desmodesmus communis, intermediate toxicity for Ankistrodesmus densus, Chlamydomonas puliminiorfes, and Raphidocelis subcapitata, and lower toxicity for Kirchneriella lunaris and Ankistrodesmus falcatus (EC50: 38, 42, 66, 103, 248, 1004, and 1585 µg L−1 atrazine, respectively). Principal component analysis (PCA) with algal characteristics suggested that the atrazine‐sensitive algae P. boryanum and D. communis were positively associated with photosynthetic levels and negatively associated with GST activity and chlorophyll‐a concentration. The PCA also suggested that the atrazine‐tolerant algae A. falcatus and K. lunaris were positively associated with morphological parameters, where the larger the cell size, the more tolerant. Although it is difficult to associate a single characteristic of algae as the key factor determining the tolerance to atrazine, results presented in this work indicate that the cell area, the photosynthetic parameters (mainly saturating irradiance), chlorophyll‐a content, and the biotransformation by GST in combination may be potential predictors for the differential tolerance of Chlorophyceae species to the herbicide. Environ Toxicol Chem 2022;41:1675–1685. © 2022 SETAC

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“…GSTs are involved in the detoxification mechanism, and 15 GST-like isoenzymes ( Cr GSTs) were found in the model species of green algae C . reinhardtii (Chatzikonstantinou et al., 2017). Transgenic GST, introduced into Arabidopsis thaliana , has been shown to increase its salt tolerance (Xu et al., 2015).…”

Section: Discussionmentioning

confidence: 99%

Potential for Heightened Sulfur-Metabolic Capacity in Coastal Subtropical Microalgae

Nelson

Chaiboonchoe²,

Fu³

et al. 2019

iScience

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SummaryThe activities of microalgae support nutrient cycling that helps to sustain aquatic and terrestrial ecosystems. Most microalgal species, especially those from the subtropics, are genomically uncharacterized. Here we report the isolation and genomic characterization of 22 microalgal species from subtropical coastal regions belonging to multiple clades and three from temperate areas. Halotolerant strains including Halamphora, Dunaliella, Nannochloris, and Chloroidium comprised the majority of these isolates. The subtropical-based microalgae contained arrays of methyltransferase, pyridine nucleotide-disulfide oxidoreductase, abhydrolase, cystathionine synthase, and small-molecule transporter domains present at high relative abundance. We found that genes for sulfate transport, sulfotransferase, and glutathione S-transferase activities were especially abundant in subtropical, coastal microalgal species and halophytic species in general. Our metabolomics analyses indicate lineage- and habitat-specific sets of biomolecules implicated in niche-specific biological processes. This work effectively expands the collection of available microalgal genomes by ∼50%, and the generated resources provide perspectives for studying halophyte adaptive traits.

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The glutathione transferase family of Chlamydomonas reinhardtii: Identification and characterization of novel sigma class-like enzymes

Cited by 18 publications

References 52 publications

Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

Enhancement of active ingredients and biological activities of Nostoc linckia biomass cultivated under modified BG-110 medium composition

Differential Effects of Atrazine on Chlorophyceae Species and Association with Morphology, Photosynthesis, Chlorophyll Content, and Glutathione‐S‐Transferase Activity

Potential for Heightened Sulfur-Metabolic Capacity in Coastal Subtropical Microalgae

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