The glycoprotein of infectious hematopoietic necrosis virus elicits neutralizing antibody and protective responses

Evaluation of a Subunit Vaccine to Infectious Hematopoietic Necrosis Virus, July 31, 1988 to September 20, 1989 Annual Report.

“…The G protein has also been found to induce protective immunity in kokanee salmon (Oncorhynchus nerka ) and rainbow trout (Oncorhynchus mykiss) fry by injection and immersion vaccination. However, it was less immunogenic in fish than the glycoprotein of rabies virus in mice because 50 times more protein was required to induce comparable immunity in fish (Engelking and Leong, 1989). A significant finding that was made in this study was that the glycoprotein from a single type of IHNV could induce protective immunity in fish against challenge with many different electrophoretic types of IHNV.…”

“…This similarity would suggest that neutralizing and binding epitopes for the IHNV G protein would be located in regions that corresponded to those found for VSV and rabies. Engelking and Leong (1989) have prepared monospecific antisera in rabbits to four purified IHNV structural proteins -N, Ml, M2 and G. They found that the glycoprotein was the only viral protein which was capable of inducing neutralizing antibodies and the antisera prepared to N, Ml and M2 did not possess neutralizing or neutralization enhancing activity. Moreover, they found that the purified G protein could block the neutralizing activity of antiserum prepared to the whole virus.…”

Mother to fry, successful transfer of immunity against infectious haematopoietic necrosis virus infection in rainbow trout

“…No difference among groups was observed when fry were challenged 54 days and 72 days after hatching (data not shown). The presence of virus in the dead fry was confirmed by the production of characteristic cytopathic changes in RTG-2 cell cultures (Engelking & Leong, 1989).…”

“…The genome of the virus consists of an unsegmented negativesense single-stranded RNA of approximately 11 kb that encodes five structural proteins and one nonvirion protein (Kurath & Leong, I985;Kurath ef al., 1985;McAllister & Wagner, 1975). A glycoprotein on the surface of IHNV is responsible for eliciting neutralizing antibodies in fish (Engelking & Leong, 1989). Immunity can be induced using either the purified virion glycoprotein, or recombinant glycoprotein produced in E. co]i (Gilmore et aI.,i988;Xu eta]., 1991).…”

“…Next, the antisera of immunized fish were tested for their capacity to block IHNV infectivity in a cultured cell infection assay similar to that described by Engelking & Leong (1989). The virus (103 p.f.u.)…”