The glycoproteins C and G are equivalent target antigens for the determination of herpes simplex virus type 1-specific antibodies

Scheper, Thomas; Saschenbrecker, Sandra; Steinhagen, Katja; Sauerbrei, Andreas; Suer, Waltraud; Meyer, Wolfgang; Schlumberger, Wolfgang; Wandinger, Klaus‐Peter

doi:10.1016/j.jviromet.2010.02.015

Cited by 14 publications

(10 citation statements)

References 36 publications

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“…Specifically, we confirm that UL44/gC shows type-specific antigenicity (27,74) and warrants further investigation as a candidate antigen for the development of improved tests. We also confirm the type specificity of US4/gG, at least for HSV-1.…”

Section: Discussionsupporting

confidence: 65%

“…There is every reason to expect that other antigens can be used to discriminate HSV-1 and -2 infections and increase the sensitivity of the test. Indeed, UL44/gC has recently been shown to be as specific as US4/gG for type-specific tests (74), indicating that gG-1 and gG-2 are not the only HSV proteins known to induce type-specific antibodies. The only rational way to approach this problem is to test individual antigens in order to identify those that are antigenically distinct.…”

Section: Discussionmentioning

confidence: 99%

“…The test has acceptable sensitivity in convalescent-phase individuals but is less sensitive in the early stages of infection (5,6), a limitation that may be overcome by measurement of IgM or the discovery of another antigen(s) that detects IgG during acute infection. Moreover, single-antigen-based tests are prone to false negatives when infected individuals lack gG-specific antibodies in their profile (74). Western blots of whole virions can also discriminate between HSV-1 and HSV-2 infections and are more sensitive than gG-based tests (5).…”

mentioning

confidence: 99%

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Discovery of Potential Diagnostic and Vaccine Antigens in Herpes Simplex Virus 1 and 2 by Proteome-Wide Antibody Profiling

Kalantari-Dehaghi

Chun

Chentoufi

et al. 2012

J Virol

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c Routine serodiagnosis of herpes simplex virus (HSV) infections is currently performed using recombinant glycoprotein G (gG) antigens from herpes simplex virus 1 (HSV-1) and HSV-2. This is a single-antigen test and has only one diagnostic application. Relatively little is known about HSV antigenicity at the proteome-wide level, and the full potential of mining the antibody repertoire to identify antigens with other useful diagnostic properties and candidate vaccine antigens is yet to be realized. To this end we produced HSV-1 and -2 proteome microarrays in Escherichia coli and probed them against a panel of sera from patients serotyped using commercial gG-1 and gG-2 (gGs for HSV-1 and -2, respectively) enzyme-linked immunosorbent assays. We identified many reactive antigens in both HSV-1 and -2, some of which were type specific (i.e., recognized by HSV-1-or HSV-2-positive donors only) and others of which were nonspecific or cross-reactive (i.e., recognized by both HSV-1-and HSV-2-positive donors). Both membrane and nonmembrane virion proteins were antigenic, although type-specific antigens were enriched for membrane proteins, despite being expressed in E. coli. Herpes simplex virus 1 (HSV-1) and HSV-2 cause significant human morbidity. HSV-2 is the causative agent of most recurrent genital herpes lesions and is sexually transmitted. Infections are often asymptomatic, and most infected individuals are unaware of the infection, yet HSV-2 is associated with an increased risk of HIV acquisition (33) and an increased risk during pregnancy of spontaneous abortion, premature birth, and perinatal herpes (12, 13). Unawareness of HSV-2 infection is also a major contributing factor to transmission to uninfected partners (64,65). In contrast, HSV-1 is usually transmitted during childhood and is found to be associated predominantly with orolabial infections (cold sores). Also, HSV-1 infection of the eye (ocular herpes) is the most common cause of infectious corneal blindness in industrialized countries. Both HSV-1 and HSV-2 establish lifelong latent infections within the dorsal root and trigeminal ganglia and are characterized by periodic reactivation and virus shedding from mucocutaneous epithelium. Owing to the different natural histories and outcomes of HSV-1 and -2 infections, accurate diagnosis of the HSV type is important for patient management and prognosis and controlling potential transmission. For example, knowing the specific HSV type can help the patient take appropriate precautions to prevent transmission of the disease to others. In particular, the identification of unrecognized HSV-2 infection can be used to carefully monitor virus shedding during pregnancy and minimize the risk of perinatal infection.Laboratory tests for HSV infection include virus culture, virus neutralization, PCR, and serological tests. Virus culture is considered the "gold standard" in the early stages of a primary infection. However, it is less sensitive during the healing stage of infection or during recurrent infections. Culture testi...

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Discovery of Potential Diagnostic and Vaccine Antigens in Herpes Simplex Virus 1 and 2 by Proteome-Wide Antibody Profiling

Kalantari-Dehaghi

Chun

Chentoufi

et al. 2012

J Virol

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“…Ideal protein microarrays should apply highly-purified antigens [19]. Since purified recombinant antigens have indicated good specificity and antigenicity in immunoassay [20]–[22], moreover antigens expressed in Escherichia coli also showed satisfying specificity [21]–[23], we designed the antigen array based on recombinant viral specific antigens expressed in E.coli and evaluated its preliminary application for detection of IgG and IgM directed against various viral antigens, including HSV1, HSV2, CMV and RV.…”

Section: Discussionmentioning

confidence: 99%

Development of Recombinant Antigen Array for Simultaneous Detection of Viral Antibodies

Liu

Huang

et al. 2013

PLoS ONE

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Protein microarrays have been developed to study antibody reactivity against a large number of antigens, demonstrating extensive perspective for clinical application. We developed a viral antigen array by spotting four recombinant antigens and synthetic peptide, including glycoprotein G of herpes simplex virus (HSV) type 1 and 2, phosphoprotein 150 of cytomegalovirus (CMV), Rubella virus (RV) core plus glycoprotein E1 and E2 as well as a E1 peptide with the optimal concentrations on activated glass slides to simultaneously detect IgG and IgM against HSV1, HSV2, CMV and RV in clinical specimens of sera and cerebrospinal fluids (CSFs). The positive reference sera were initially used to measure the sensitivity and specificity of the array with the optimal conditions. Then clinical specimens of 144 sera and 93 CSFs were tested for IgG and IgM antibodies directed against HSV1, HSV2, CMV and RV by the antigen array. Specificity of the antigen array for viral antibodies detection was satisfying compared to commercial ELISA kits but sensitivity of the array varied relying on quality and antigenic epitopes of the spotting antigens. In short, the recombinant antigen array has potential to simultaneous detect multiple viral antibodies using minute amount (3 µl) of samples, which holds the particularly advantage to detect viral antibodies in clinical CSFs being suspicious of neonatal meningitis and encephalitis.

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“…Type-specific epitopes are present on the surface glycoproteins gG (HSV-1 and HSV-2) 4 and gC (HSV-1). 5 …”

Section: Herpes Simplex Virusmentioning

confidence: 99%

Optimal management of genital herpes: current perspectives

Sauerbrei¹

2016

IDR

Self Cite

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As one of the most common sexually transmitted diseases, genital herpes is a global medical problem with significant physical and psychological morbidity. Genital herpes is caused by herpes simplex virus type 1 or type 2 and can manifest as primary and/or recurrent infection. This manuscript provides an overview about the fundamental knowledge on the virus, its epidemiology, and infection. Furthermore, the current possibilities of antiviral therapeutic interventions and laboratory diagnosis of genital herpes as well as the present situation and perspectives for the treatment by novel antivirals and prevention of disease by vaccination are presented. Since the medical management of patients with genital herpes simplex virus infection is often unsatisfactory, this review aims at all physicians and health professionals who are involved in the care of patients with genital herpes. The information provided would help to improve the counseling of affected patients and to optimize the diagnosis, treatment, and prevention of this particular disease.

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The glycoproteins C and G are equivalent target antigens for the determination of herpes simplex virus type 1-specific antibodies

Cited by 14 publications

References 36 publications

Discovery of Potential Diagnostic and Vaccine Antigens in Herpes Simplex Virus 1 and 2 by Proteome-Wide Antibody Profiling

Discovery of Potential Diagnostic and Vaccine Antigens in Herpes Simplex Virus 1 and 2 by Proteome-Wide Antibody Profiling

Development of Recombinant Antigen Array for Simultaneous Detection of Viral Antibodies

Optimal management of genital herpes: current perspectives

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