This study investigates the post-regulation scenario of waste electrical and electronic equipment (e-waste) reverse logistics in Florianópolis, an island in southern Brazil, following Decree N° 10240/2020. Employing a case study approach, involving observation and semi-structured interviews, the research explores key stakeholders’ roles. The results indicate collaborative efforts among the municipal cleaning service provider, recycler, and manager entity, supporting effective e-waste take-back systems. This cooperation not only strengthens the formal recycling market but also yields socio-environmental benefits. The study reveals that optimal outcomes arise from the collaboration between the public cleaning service provider and the business sector, fostering a mutually beneficial relationship. Concerning e-waste value recovery, there is a notable inclination to extend the life cycle of small equipment, emphasizing reuse and remanufacture cycles, while larger electrical and electronic items follow to recycling and recovery cycles. In summary, this research contributes to aligning stakeholders in e-waste reverse logistics, emphasizing compliance with legislation and fostering a deeper understanding of roles, functions, and alliances. The study provides a strategic and structured perspective on e-waste management in a city renowned for selective waste collection and considered a national benchmark for reverse logistics, reinforcing its commitment to sustainability.