The GPI-Anchored Protein Thy-1/CD90 Promotes Wound Healing upon Injury to the Skin by Enhancing Skin Perfusion

Abstract

Wound healing is a highly regulated multi-step process that involves a plethora of signals. Blood perfusion is crucial in wound healing and abnormalities in the formation of new blood vessels define the outcome of the wound healing process. Thy-1 has been implicated in angiogenesis and silencing of the Thy-1 gene retards the wound healing process. However, the role of Thy-1 in blood perfusion during wound closure remains unclear. We proposed that Thy-1 regulates vascular perfusion, affecting the healing rate in mouse skin. We analyzed the time of recovery, blood perfusion using Laser Speckle Contrast Imaging, and tissue morphology from images acquired with a Nanozoomer tissue scanner. The latter was assessed in a tissue sample taken with a biopsy punch on several days during the wound healing process. Results obtained with the Thy-1 knockout (Thy-1−/−) mice were compared with control mice. Thy-1−/− mice showed at day seven, a delayed re-epithelialization, increased micro- to macro-circulation ratio, and lower blood perfusion in the wound area. In addition, skin morphology displayed a flatter epidermis, fewer ridges, and almost no stratum granulosum or corneum, while the dermis was thicker, showing more fibroblasts and fewer lymphocytes. Our results suggest a critical role for Thy-1 in wound healing, particularly in vascular dynamics.

Impact of Canine Amniotic Mesenchymal Stem Cell Conditioned Media on the Wound Healing Process: In Vitro and In Vivo Study

“…Next, high expression of CD29 and CD44 is superior to strong chondrogenic potential [31]. For our experiment, the expression of CD90/Thy-1 plays an important role, because it is implicated in angiogenesis and blood perfusion during wound closure [32]. Concluding from the above-mentioned facts, amnion tissue represents a relatively rich source of stem cells.…”

Analyzing secretory proteins in human dermal fibroblast‐conditioned medium for angiogenesis: A bioinformatic approach

“…Thy‐1, also known as CD90, is a glycosyl phosphatidyl inositol‐anchored protein with implications in angiogenesis and vascular perfusion during wound repair 34 . Furthermore, Thy‐1‐positive fibroblasts within the wound environment have been shown to enhance tissue contraction and fibrotic activity 35 .…”

“…33 Thy-1, also known as CD90, is a glycosyl phosphatidyl inositolanchored protein with implications in angiogenesis and vascular perfusion during wound repair. 34 Furthermore, Thy-1-positive fibroblasts within the wound environment have been shown to enhance tissue contraction and fibrotic activity. 35 TGF-β-induced protein ig-h3, which is a collagen-binding matrix protein, has been found to be essential in the sprouting and lumen formation of endothelial cells during angiogenesis, in conjunction with other fibroblast-derived matrix proteins, including collagen α1, procollagen C-endopeptidase enhancer 1, and IGFBP-7.…”

Serum lipidomics‐based study of electroacupuncture for skin wound repair in rats

“…The new blood vessels formed by repair can promote tissue blood perfusion and is a key factor in determining the quality of skin wound healing. Therefore, blood perfusion is essential in skin wound healing 10,11 . Human skin homeostasis requires the regulation of lipid metabolic balance and lipids secreted by sebocytes together with lipids derived from keratin‐forming cells form an important component of the skin barrier 12 .…”

“…Therefore, blood perfusion is essential in skin wound healing. 10 , 11 Human skin homeostasis requires the regulation of lipid metabolic balance and lipids secreted by sebocytes together with lipids derived from keratin‐forming cells form an important component of the skin barrier. 12 Chromatography‐mass spectrometry (LC–MS) combines the capabilities of both HPLC and MS, significantly improving the analytical platform and providing a sensitive and specific tool for the identification and quantification of lipids.…”