The graft framework: Quantitative changes in cell wall matrix polysaccharides throughout the tomato graft union formation

Frey, Carlos; Manga-Robles, Alba; Acebes, José Luis; Encina, Antonio

doi:10.1016/j.carbpol.2021.118781

Cited by 11 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results also found that SpuXTH16, SpuXTH17, SpuXTH25, PtrXTH17 and PtrXTH22 highly expressed at 3-28 days after poplar and willow self-grafted combinations. The up-regulated XTH genes may lead to the accumulation of xyloglucan in the cell wall of tomato graft interface to promote cell adhesion [17] . Cell wall modi cation related genes actively expressed, causing signi cant changes in the cell wall to promote the formation of secondary plasmodesmata at the grafted junction [49] .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide identification and expression analysis of xyloglucan endotransglucosylase/hydrolase genes family in Salicaceae during grafting

Yang,

Chen,

Liu

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Poplar and willow are important fast-growing trees in China. Grafting plays an important role in improving plant stress resistance and construction of ornamental plants. It is found that willow scions grafted onto poplar rootstocks can form ornamental plants. However, this grafted combination has a low survival rate. Many studies have reported that the xyloglucan endotransglucosylase/hydrolase (XTH) family plays an important role in the healing process of grafts. Results: A total of 38 PtrXTHs and 32 SpuXTHs were identified in poplar and willow respectively, and were classified into three subfamilies. Tandem duplication was the main reason for the expansion of the PtrXTHs. Grafting treatment and Quantitative real time PCR (RT-qPCR) analysis revealed that five XTHgenes differentially expressed between self-grafted and reciprocal grafted combinations. Specifically, the high expression levels of SpuXTH16, SpuXTH17, SpuXTH25, PtrXTH22 and PtrXTH17 may contribute to the high survival rate of the grafted combination with willow scion and poplar rootstock. Subcellular localization identified that the SpuXTH16, SpuXTH17, SpuXTH25, PtrXTH17 and PtrXTH22 proteins were located on the cell walls. Transcription factors (NAC, MYB and DOF) may regulate the five XTH genes. Conclusions: This study provides a new understanding of the roles of PtrXTH and SpuXTHgenes and their roles in grafting. Our results will give some hints to explore the molecular mechanisms of PtrXTH and SpuXTH genes involved in grafting in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

“…RNA-seq analyses also indicated that cell wall-related genes played signi cant roles in promoting plant grafting healing [13][14][15][16] . It is believed that cell wall modi cation/reconstruction enzymes positively participate in the entire grafting process to regulate new cell wall establishment and expansion [17,18] .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of xyloglucan endotransglucosylase/hydrolase genes family in Salicaceae during grafting

Yang,

Chen,

Liu

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The formation of the graft union is a dynamic and continuous phenomenon involving a complex series of events at the physiological, cellular and molecular levels that are necessary to ensure the successful union between rootstock and scion (Fernández-García et al, 2004;Frey et al, 2022Frey et al, , 2023Melnyk et al, 2015). In addition, grafting is an important research tool for understanding how different physiological mechanisms are coordinated in a wide range of plants (Notaguchi et al, 2020;Reeves et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Non‐invasive monitoring of tomato graft dynamics using thermography and fluorescence quantum yields measurements

Frey,

Hernández‐Barriuso,

Encina

et al. 2023

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Grafting involves a sequence of modifications that may vary according to genotypes, grafting techniques and growing conditions. This process is often monitored using destructive methods, precluding the possibility of monitoring the entire process in the same grafted plant. The aim of this study was to test the effectiveness of two noninvasive methods-thermographic inference of transpiration and determination of chlorophyll quantum yields-for monitoring graft dynamics in tomato (Solanum lycopersicum L.) autografts and to compare the results with other reliable measures: mechanical resistance parameters and xylem water potential. The mechanical resistance of grafted

show abstract

“…arabinogalactan proteins (AGPs) (Zhang et al 2021 ). In tomato stems, as well as in other dicot, the major matrix cell wall polysaccharides are xylan and xyloglucan, among the hemicelluloses, and homogalacturonan (HG) and rhamnogalacturonan I (RGI) and II, among the pectins (Zhang et al 2021 ; Frey et al 2022 ). When certain cell types stop growing they accumulate a secondary cell wall composed basically by cellulose, xylans and lignin (Zhong et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…It is considered that cell walls have an important role in grafting process (Miller and Barnett 1993 ; Yeoman 1993 ; Pitaksaringkarn et al 2014 ; Melnyk 2017 ; Frey et al 2021 , 2022 ). In fact, transcriptomic analysis in tomato and other species has shown that significant overexpression of genes for cell wall biosynthesis and remodelling occurs early after grafting onset (Cookson et al 2013 ; Cui et al 2021 ; Xie et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical dynamics of cell wall matrix polymers during tomato autograft healing

et al. 2023

Self Cite

View full text Add to dashboard Cite

A large part of the production of tomato plants is grafted. Although it has recently been described that cell walls play an important role in tomato graft healing, the spatiotemporal dynamics of cell wall changes in this critical process remains largely unknown. The aim of this work was to immunolocalize changes in the major cell wall matrix components of autograft union tissues throughout the course of healing, from 1 to 20 days after grafting (DAG). Homogalacturonan was de novo synthetized and deposited in the cut edges, displaying the low methyl-esterified homogalacturonan a stronger labelling. Labelling of galactan side chains of rhamnogalacturonan increased until 8 DAG, although remarkably a set of cells at the graft union did not show labelling for this epitope. Changes in xylan immunolocalization were associated to the xylem vasculature development throughout, while those of xyloglucan revealed early synthesis at the cut edges. Arabinogalactan proteins increased up to 8 DAG and showed scion-rootstock asymmetry, with a higher extent in the scion. The combination of these changes appears to be related with the success of the autograft, specifically facilitating the adhesion phase between scion-rootstock tissues. This knowledge paves the way for improved grafting using methods that facilitate appropriate changes in the time and space dynamics of these cell wall compounds.

show abstract

The graft framework: Quantitative changes in cell wall matrix polysaccharides throughout the tomato graft union formation

Cited by 11 publications

References 34 publications

Genome-wide identification and expression analysis of xyloglucan endotransglucosylase/hydrolase genes family in Salicaceae during grafting

Genome-wide identification and expression analysis of xyloglucan endotransglucosylase/hydrolase genes family in Salicaceae during grafting

Non‐invasive monitoring of tomato graft dynamics using thermography and fluorescence quantum yields measurements

Immunohistochemical dynamics of cell wall matrix polymers during tomato autograft healing

Contact Info

Product

Resources

About