The graft response to transplantation: a gene expression profile analysis

Christopher, Kenneth B.; Mueller, Thomas; DeFina, Rachel; Liang, Yurong; Zhang, Jianhua; Gentleman, Robert; Perkins, David L.

doi:10.1152/physiolgenomics.00139.2002

Cited by 13 publications

(8 citation statements)

References 24 publications

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“…The induction of tolerance to the graft would be the treatment of choice after transplantation. Increased expression of protective genes like metallothionein-1 or α2β- crystallin were reported with a mouse cardiac transplant model [ 36 , 37 ]. In this same context, we observed increased expression of a number of genes known to have protective or regulatory properties (Figs.…”

Section: Discussionmentioning

confidence: 99%

Activation of counter-regulatory mechanisms in a rat renal acute rejection model

et al. 2008

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Section: Discussionmentioning

confidence: 99%

Activation of counter-regulatory mechanisms in a rat renal acute rejection model

et al. 2008

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“…Investigation of effector mechanisms of graft-infiltrating cells by utilizing rat and mouse models of adoptive transfer followed by HHTx surgery has led to a wealth of information regarding immunopathological mechanisms. A sample of the literature includes: determining the critical role of CD4+ and CD8+ T lymphocytes in stimulating graft rejection and their mechanistic roles (50-52); the potential therapeutic effect of antagonism of chemokine (CCR5) and chemokine receptors (CXCR3) in inhibiting both acute and chronic allograft rejection (48); understanding the association of increased Fas ligand mRNA expression with myocardial apoptosis and ischemia-reperfusion injury and the eventual progression to chronic rejection (53); and studying the post-transplant shift in expression of genes classified as defense, communication, and metabolism during the graft response to transplantation injury and rejection (54). For further reading on this large topic, a recent review by Wehner et al is worth consulting (55).…”

Section: Graft Surveillance and Rejectionmentioning

confidence: 99%

The 'Mighty Mouse' Model in Experimental Cardiac Transplantation

Bishay¹

2011

HYP

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Heterotopic Vascularized Cardiac Grafts in Rodents Heterotopic transplantation was used as early as the 1940's and 50's in large animals but it wasn't until work by Abbott et al. in 1964 that the heterotopic heart transplantation (HHTX) was perfomed in rodents; the authors used rats and utilized end-to-end anastomosis of the donor thoracic aorta to the recipient abdominal aorta and donor pulmonary artery to recipient inferior vena cava (IVC) (10). Abbott's procedure resulted in restricted blood flow to the lower extremities and consequently, paraparesis and paraplegia (1). This procedure was revised by Ono and Lindsey to allow for greater blood flow to the lower extremities and employed end-to-side anastomoses of the donor vessels to the recipient aorta and IVC, resulting in 90% graft and recipient survival (11). Heterotopic cervical transplantation was performed in rats in 1971 (12) and required the anastomosis of the re

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“…To address these limitations, the effects of various interventions on grafted organs in mouse and rat models have been assessed using expression microarrays. The overall dynamics of gene expression after cardiac transplant in mice (48)(49)(50)(51) and rats (52, 53) have been assessed. A collection of cytotoxic T-lymphocyte-associated transcripts leading to the development of tubulitis was found in mouse kidney allografts (54).…”

Section: Microarray Gene Expression Analysis Of Nonhuman Transplant Mmentioning

confidence: 99%

Novel Insights into Lung Transplant Rejection by Microarray Analysis

Lande

Patil

Li³

et al. 2007

Proceedings of the American Thoracic Society

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Gene expression microarrays can estimate the prevalence of mRNA for thousands of genes in a small sample of cells or tissue. Organ transplant researchers are increasingly using microarrays to identify specific patterns of gene expression that predict and characterize acute and chronic rejection, and to improve our understanding of the mechanisms underlying organ allograft dysfunction. We used microarrays to assess gene expression in bronchoalveolar lavage cell samples from lung transplant recipients with and without acute rejection on simultaneous lung biopsies. These studies showed increased expression during acute rejection of genes involved in inflammation, apoptosis, and T-cell activation and proliferation. We also studied gene expression during the evolution of airway obliteration in a murine heterotopic tracheal transplant model of chronic rejection. These studies demonstrated specific patterns of gene expression at defined time points after transplantation in allografts, whereas gene expression in isografts reverted back to that of native tracheas within 2 wk after transplantation. These studies demonstrate the potential power of microarrays to identify biomarkers of acute and chronic lung rejection. The application of new genetic, genomic, and proteomic technologies is in its infancy, and the microarray-based studies described here are clearly only the beginning of their application to lung transplantation. The massive amount of data generated per tissue or cell sample has spawned an outpouring of invention in the bioinformatics field, which is developing methodologies to turn data into meaningful and reproducible clinical and mechanistic inferences. Keywords: allograft rejection; lung transplantation; microarrayLung and heart-lung transplants, introduced into clinical practice in 1981, have now been performed in over 20,000 individuals worldwide for whom effective medical therapy was not available (1). These procedures have been highly beneficial for many recipients, with 2-yr survival rates of approximately 70% and dramatic improvements in quality of life. Despite these remarkable successes, problems remain, and long-term survival rates for lung transplant recipients are considerably lower than those observed in kidney, heart, and liver recipients. This is due, in large part, to the development of chronic allograft rejection despite administration of immunosuppressive medications.

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The graft response to transplantation: a gene expression profile analysis

Cited by 13 publications

References 24 publications

Activation of counter-regulatory mechanisms in a rat renal acute rejection model

Activation of counter-regulatory mechanisms in a rat renal acute rejection model

The 'Mighty Mouse' Model in Experimental Cardiac Transplantation

Novel Insights into Lung Transplant Rejection by Microarray Analysis

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