The grain market of Russia

Agapkin, A.M.; Makhotina, I. A.

doi:10.1088/1755-1315/839/2/022023

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…В 2019-2020 гг. Россия экспортировала зерно по кодам ТН ВЭД 1001 (пшеница и меслин), 1002 (рожь), 1003 (ячмень), 1004 (овес) на сумму около 9,5 млрд долларов, что сделало ее крупнейшим экспортером пшеницы в мире [1]. Основными импортерами зерна из России были Саудовская Аравия, Египет, Турция, Бангладеш, Иран, Нигерия, Китай и Пакистан, из которых Турция импортировала 9, а Саудовская Аравия 3,2 млн тонн в 2020 г.…”

Section: Introductionunclassified

“…Основными импортерами зерна из России были Саудовская Аравия, Египет, Турция, Бангладеш, Иран, Нигерия, Китай и Пакистан, из которых Турция импортировала 9, а Саудовская Аравия 3,2 млн тонн в 2020 г. [1]. Фитосанитарными требованиями указанных стран регулируются один или несколько возбудителей бактериозов зерновых культур, принадлежащих следующим видам: Pseudomonas fuscovaginae, Pseudomonas syringae и Xanthomonas translucens [2,3].…”

Section: Introductionunclassified

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Identification of Pseudomonas fuscovaginae, Pseudomonas syringae and Xanthomonas translucens in wheat seeds using PCR

Muvingi

Slovareva²,

Zargar³

2022

Vestn. Ross. univ. družby nar., Ser. Agron. životnovod.

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The causative agents of grain crops bacteriosis viz. Pseudomonas fuscovaginae , Pseudomonas syringae and Xanthomonas translucens are regulated by phytosanitary requirements of the largest importers of Russian grain - Egypt, Turkey, Bangladesh, Nigeria and Pakistan. Therefore, it requires the development of rapid methods for their diagnosis. The PCR method, which is the fastest and most reliable in testing laboratories, needs optimal preparation of the test material. The aim of the study was to optimize the process of preparing seed samples for subsequent detection and identification of P. fuscovaginae, P. syringae and X. translucens by PCR. Wheat grain samples were soaked in phosphate-buffered saline (PBS) for 2 hours and infected with suspensions of P. fuscovaginae, P. syringae pv. coronafaciens and X. translucens at various concentrations. Then, the infected grain samples were crushed and subjected to two-stage centrifugation. DNA was isolated from the obtained analytical samples and species-specific PCR was performed for each bacterial species. It was found that a two-hour soaking of the seeds and their treatment with a homogenizer is sufficient to effectively destroy each grain in the sample and ensure the release of bacteria into the liquid part of the sample. The first low-speed centrifugation allowed the crushed grain to settle efficiently and remove excess starch from the supernatant. High-speed centrifugation of the supernatant made it possible to obtain a concentrated microbiota contained in the grain sample. To obtain DNA of sufficient quality for PCR test, the kit Proba-GS (AgroDiagnostika, Russia) was used for DNA extraction. Using Pseudomonas fuscovaginae-RT kit (Syntol, Russia) and PsyF/PsyR and 4F1/4R 1 primers, DNA of P. fuscovaginae P. syringae and X. translucens , respectively, was successfully detected in each of the samples infected with these bacteria at concentrations of 103 CFU/ml. The absence of PCR inhibition was noted. The method of removing starch from samples for molecular diagnostics of phytopathogens was used for the first time. Application of these methods will allow diagnosing pathogens of bacterioses within one day.

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Section: Introductionunclassified

Identification of Pseudomonas fuscovaginae, Pseudomonas syringae and Xanthomonas translucens in wheat seeds using PCR

Muvingi

Slovareva²,

Zargar³

2022

Vestn. Ross. univ. družby nar., Ser. Agron. životnovod.

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On the requirements of the technical regulation of the Customs Union 015/2011 “On the Safety of Grain” for the identification of grain

Oshmarina,

Agapkin

2022

Commodity specialist of food products

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Grain and its processed products are an integral part of economic development and stable and safe functioning of the food market. It is necessary to supply high-quality grain for sale, in respect of which the manufacturer must comply with all the requirements of the current legislation of the Russian Federation. The state controls the quality according to Technical Regulations of the Customs Union 015/2011 “On the safety of grain”, which establishes mandatory requirements for grain and procedures related to grain and processed products for their circulation on the territory of the EAEU Customs Unio

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To the 140th anniversary of the birth of Professor Vasiliy Stepanovich Smirnov

Agapkin¹,

Rodina²

2022

Commodity specialist of food products

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This year marks the 140th anniversary of the birth of Vasiliy Stepanovich Smirnov (1882–1958), PhD in Engineering, professor, honored worker of science and technology of the RSFSR. V.S. Smirnov began his teaching career in 1907 at the Moscow Industrial School, which was then reorganized into the D.I. Mendeleev Institute of Chemical Technology. Since 1908, he taught chemistry and commodity science in trade schools, and since 1913 — at the Moscow Commercial Institute (later the Plekhanov Moscow Institute of National Economy), now the Plekhanov Russian University of Economics. For more than 45 years, he taught a course in commodity science of grain and flour products at the Plekhanov Moscow Institute of National Economy, where he organized a grain laboratory and for 10 years, from 1947 to 1957 he was the head of the Department of Commodity Science. For the first time in 1915, he wrote the section “Cereal Bread, Flour and Wheat Bread” in the textbook “Food Products”. In 1938, he wrote “Grain Products”, a four-volume textbook for higher educational institutions, and when it was reprinted in 1950, he was also the editor-in-chief of the entire textbook. In his research work, V.S. Smirnov pursued the goal of organizing technochemical control at processing enterprises to stabilize the quality of flour and cereals. He published more than 75 scientific articles, and 15 PhD theses were defended under his supervision. Vasiliy Stepanovich was a well-rounded specialist; his lectures were characterized by depth of content, practical orientation, and accessibility in acquisition. He was an excellent methodologist, and the textbooks prepared by him have not lost their significance up to date.

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The grain market of Russia

Cited by 4 publications

References 5 publications

Identification of Pseudomonas fuscovaginae, Pseudomonas syringae and Xanthomonas translucens in wheat seeds using PCR

Identification of Pseudomonas fuscovaginae, Pseudomonas syringae and Xanthomonas translucens in wheat seeds using PCR

On the requirements of the technical regulation of the Customs Union 015/2011 “On the Safety of Grain” for the identification of grain

To the 140th anniversary of the birth of Professor Vasiliy Stepanovich Smirnov

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