The granulocytes of fish

Hine, P. M.

doi:10.1016/s1050-4648(05)80038-5

Cited by 82 publications

(59 citation statements)

References 144 publications

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“…In fish, neutrophils and macrophages operate in infectious situations through processes essentially identical to those reported for mammals [1,24,36,[39][40][41]. Indeed, the interaction between neutrophils and macrophages in peritoneal inflammatory exudates in rainbow trout injected intraperitoneally with bacterial and non-bacterial phlogistic agents has been studied extensively by light and electron microscopy [36].…”

Section: Discussionmentioning

confidence: 95%

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

Harun

Zou

Zhang

et al. 2008

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 95%

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

Harun

Zou

Zhang

et al. 2008

Developmental & Comparative Immunology

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“…process in the defense of vertebrates against some infections (Mackaness 1969, Ellis 1977 Hine 1992, Brown 1995. It is interesting that mammals, teleosts and other higher eukaryotes utilize 2 types of phagocytic cells-neutrophils and macro- 'E-mail: aafonso@gaia.ibmc.up.pt phages-in the defense against infection (Rowley et al 1988, Secombes & Fletcher 1992, Brown 1995.…”

Section: Introductionmentioning

confidence: 99%

Neutrophil and macrophage responses to inflammation in the peritoneal cavity of rainbow trout Oncorhynchus mykiss. A light and electron microscopic cytochemical study

Afonso

Lousada²,

Silva³

et al. 1998

Dis. Aquat. Org.

145

124

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The neutrophil and macrophage responses that accompany inflammation were studied in the peritoneal cavity of rainbow trout Oncorhynchus mykiss using light and electron microscopic cytochemistry. Neutrophils of inflamnlatory pentoneal exudates were alpha-naphthyl butyrate esterasenegative, peroxldase-positive and rich in cytoplasmic glycogen granules. Macrophages were poor in glycogen, esterase-positive and usually peroxidase-negative. Some peroxidase-posibve macrophages were due to the transfer to macrophages of neutrophilic peroxidase. The ultrastructural double labehng for glycogen/peroxidase or esterase/peroxidase was most useful for precisely characterising neutrophils and macrophages in the inflamed peritoneal cavities and for correctly labelling peroxidasepositive macrophages. Intraperitoneal injection of casein, Incomplete Freund's Adjuvant (IFA) and live or formol-killed Yersinia ruckeri resulted in a rapid influx of neutrophils, peaking at 24 to 48 h postinjection and reaching values, in the case of live bacteria, 5OOx those in the resting, unstimulated peritoneal cavity. Peritonea1 macrophages also increased, but the response was slower (peak at 5 d ) and with more modest increases in number ( 7 . 5~) Neutrophil and mononuclear cells returned to normal values after 15 d in the case of casein and bacteria, but continued above base values 30 d after the injection of IFA. Conversely, after the injection of phosphate buffered s a h e , India ink or with shaminjections, very moderate neutrophil and macrophage responses subsided in a few hours. Phagocytosis of bacteria was studied by light microscopy of preparations stained for peroxidase by a new method which allows for the simultaneous observation of intracellular bacteria and peroxidase staining. When Y. ruckeri was injected into resting peritoneal cavities, bacteria were ingested by the resident macrophages. When the bacteria were injected into cavities with high numbers of neutrophils (due to the previous injection of casein), more neutrophils than macrophages contained bacteria. Results show that the macrophages are the resident phagocytes of the peritoneal cavity of trout, while neutrophils are present in that body cavity in significant numbers only in situations of inflammation and only as long as the inflammation persists.

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“…As indicated in a number of recent reviews (Blazer 1991, Ainsworth 1992, Hine 1992, Secombes & Fletcher 1992, the phagocytic behavior of both macrophages and neutrophils plays an important role in the nonspecific defense mechanisms of teleost fish. Although the cytological characteristics and phagocytic nature of both these cell types have been extensively studied in certain groups of fishes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural observations on the phagocytic behavior of winter flounder Pleuronectes americanus peritoneal neutrophils and macrophages in vivo

Je¹,

Ra²

1996

Dis. Aquat. Org.

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ABSTRACT. A qualitative electron microscopical examination of perltoneal phagocytes in winter flounder Pleuronectes americanus after brief (i.e. 30 or 60 min) exposure to formalin-killed Bacillus cereus or Yersinia ruckerj revealed that both neutrophils and macrophages had phagocytozed bacteria. Neutrophils obtained from both the perltoneal cavity and from the head kidney were identified on the basis of their overall cytological appearance and more specifically on the presence of elongated, striated (fibrillar) appearing granules in their cytoplasm which stained positively for peroxidase. Macrophages recovered from the peritoneal cavity lvere phagocytic like neutrophils; however, they were not observed to contain peroxidase. The surface membrane changes observed for 'activated' neutrophils and macrophages which had engulfed bacteria were consistent \\rith those reported for other fish species and higher vertebrates. Images of aggregated cells suggestive of cellular communication between lymphocytes, macrophages, and/or neutrophils are provided.

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The granulocytes of fish

Cited by 82 publications

References 144 publications

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8

Neutrophil and macrophage responses to inflammation in the peritoneal cavity of rainbow trout Oncorhynchus mykiss. A light and electron microscopic cytochemical study

Ultrastructural observations on the phagocytic behavior of winter flounder Pleuronectes americanus peritoneal neutrophils and macrophages in vivo

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