The green alga <i>Dictyosphaerium chlorelloides</i> biomass and polysaccharides production determined using cultivation in crossed gradients of temperature and light

Kumar, Dhanesh; Kvíderová, Jana; Kaštánek, Petr; Lukavský, Jaromír

doi:10.1002/elsc.201700014

Cited by 47 publications

(13 citation statements)

References 60 publications

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“…The EPS production commonly found in Chlorophyta species ranges from 0.38 (Halaj et al 2018) to 0.94 g dw L −1 (Mishra et al 2011). Higher EPS rates to that recorded in this work have been reported for some algal strains such as Gyrodinium impudicum (1.347 g dw L −1 , Yim et al 2004), Porphyridium marinum (2.5 g dw L −1 , Soanen et al 2016), and Dictyosphaerium chlorelloides (2.1 g dw L −1 , Kumar et al 2017).…”

Section: Biomass and Eps Productionsupporting

confidence: 48%

Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp.

Gongi

Cordeiro

Pinchetti³

et al. 2020

J Appl Phycol

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The present study developed a two-step strategy to enhance the production of extracellular polymeric substances (EPSs) by a thermotolerant chlorophyte, Graesiella sp., in view to their industrial valorisation. In the first step, Graesiella sp. was grown in outdoor conditions in pilot-scale photobioreactors of 100 L culture volumes. In the second step, the biomass collected in the exponential growth phase was submitted to heat stress (50°C). A joint production of biomass reaching 0.50 g dw L −1 day −1 and of EPS production reaching 1.30 g dw L −1 in 2 days was obtained. EPSs mainly contained polysaccharides (80%) and proteins (14%). FTIR and 1 HNMR revealed the presence of primary amine and sulfated groups. The EPSs contained antioxidant enzymes (SOD, CAT, and APX) maintained in an active state by the microenvironment offered by the EPSs. The EPSs were found to have a potent antioxidant activity via directly scavenging free radicals when compared to L-ascorbic acid.

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Section: Biomass and Eps Productionsupporting

confidence: 48%

Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp.

Gongi

Cordeiro

Pinchetti³

et al. 2020

J Appl Phycol

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“…Higher biomass concentrations and growth rates were achieved in heterotrophic and mixotrophic (photoheterotrophic) conditions [ 23 ]. As with other microalgae, in which light is reported to affect the production of carotenoids, polysaccharides and bioactive molecules [ 24 , 25 , 26 , 27 ], the presence or absence of light affects cell composition in E . gracilis , so that mixotrophic growth continues to be of interest in E .…”

Section: Introductionmentioning

confidence: 94%

Euglena gracilis growth and cell composition under different temperature, light and trophic conditions

et al. 2018

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BackgroundEuglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds. The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids). Comparisons were primarily carried out in cultures grown at 23 °C, but the effect of growth at higher temperatures (27 or 30 °C) was also considered.Cell growthSpecific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer. Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions. Temperature had less effect on cell composition.Cell compositionAlthough E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon. These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected. The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated. The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically. Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells. α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark. Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass. Light was also important for the production of vitamin E and phytol.

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“…However, TP in water and periphyton was not significantly different at 15 • C, suggesting that the effect of illumination on the development of periphyton was temperature dependent. This could be related to a different physiological acclimation of different algal species to different environmental conditions [26,27].…”

Section: Discussionmentioning

confidence: 99%

Capture and Release of Phosphorus by Periphyton in Closed Water Systems Influenced by Illumination and Temperature

Zhao

Chen

Xiong

et al. 2019

Water

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Periphyton is known to play an important role in the self-purification of aquatic ecosystems. However, little attention has been paid to the understanding of P distribution and its partitioning influenced by the physical parameters when periphyton is separated from the sediment. In this work, the effect of periphyton on the capture and release of phosphorus in closed water systems was studied and the influence of illumination and temperature conditions were investigated. Results showed that phosphorus was transferred from water to periphyton during the experiment at 15 °C, but periphyton turned from a sink to a source of phosphorus in a few days at 25 and 35 °C. Phosphorus capture in periphyton was more enhanced when illuminated at 70 than 20 μmol photons m−2 s−1 at 25 and 35 °C, but not at 15 °C. At the end of the experiment, cyanobacteria became more abundant at 25 and 35 °C and phosphorus fractionation showed that labile-P was predominant in periphyton. The release of the captured phosphorus could be related to the disaggregation of periphyton following the depletion of nutrients. Therefore, periphyton act as a temporary storage of phosphorus following nutrient input in closed water systems and the capture and release of phosphorus is strongly influenced by the environmental conditions.

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The green alga Dictyosphaerium chlorelloides biomass and polysaccharides production determined using cultivation in crossed gradients of temperature and light

Cited by 47 publications

References 60 publications

Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp.

Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp.

Euglena gracilis growth and cell composition under different temperature, light and trophic conditions

Capture and Release of Phosphorus by Periphyton in Closed Water Systems Influenced by Illumination and Temperature

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