The green microalgae Tetradesmus obliquus (Scenedesmus acutus) as lectin source in the recognition of ABO blood type: purification and characterization

Silva, A. J.; Cavalcanti, Vivianne Lays Ribeiro; Porto, Tatiana Souza; Gama, Watson Arantes; Costa, Romero Marcos Pedrosa Brandão; Bezerra, Raquel Pedrosa

doi:10.1007/s10811-019-01923-5

Cited by 20 publications

(9 citation statements)

References 37 publications

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“…The non-enzymatic lectins with unique sugar-binding characteristics are ubiquitous proteins capable of reversible discrete interaction with cell surface carbohydrate associated structures and involved in diverse biological and pathological functions (He et al 2015;Muszyńska et al 2018;Nascimento et al 2020;Perduca et al 2020). Aside from being a remarkable tool in blood group determination, lectins also perform defense-related functions (Silva et al 2020). Although originally identified in plants and animals, exploration of fungal lectins is becoming popular because of their peculiar carbohydrate specificity coupled with potential application in biotechnology and biomedicine (Singh et al 2010;Varrot et al 2013;Hassan et al 2015;Singh et al 2015).…”

Section: Introductionmentioning

confidence: 99%

A puriﬁed lectin with larvicidal activity from a woodland mushroom, Agaricus semotus Fr.

Adedoyin

Adewole²,

Agunbiade

et al. 2021

Acta Biol Szeged

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This study investigated the larvicidal activity on Culex quinquefasciatus of lectin purified from fresh fruiting bodies of woodland mushroom, Agaricus semotus. A. semotus lectin (ASL) was purified via ion-exchange chromatography on DEAE-cellulose A-25 and size exclusion chromatography on Sephadex G-100 matrix. Molecular weight (16.6 kDa) was estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The eﬀects of temperature, pH, metal chelation- and larvicidal activity of ASL were also investigated. The ASL indiﬀerently agglutinated the erythrocytes of the human ABO blood system and was stable at acidic pH and below 50 °C whereas 66% of its activity was lost at 60 °C with complete inactivation at 70 °C. ASL is a metalloprotein requiring barium ion as chelation of metals by 50 mM EDTA rendered the lectin inactive, while the addition of BaCl2, among other metal salts, restored the activity. ASL showed larvicidal activity against C. quinquefasciatus larvae after 24 h with a mortality of 5 and 95% at 5 and 25 mg/mL respectively, and LC50 of 13.80 mg/mL. This study concluded that purified A. semotus lectin showed impressive larvicidal activity, which could be exploited in its development as an insecticidal agent.

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Section: Introductionmentioning

confidence: 99%

A puriﬁed lectin with larvicidal activity from a woodland mushroom, Agaricus semotus Fr.

Adedoyin

Adewole²,

Agunbiade

et al. 2021

Acta Biol Szeged

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“…Regardless of the different protein concentrations in the two extracts compared to what was found in the starting biomass, it is known that in microalgae, the major proteins are Rubisco and LHCII [ 9 , 59 ], but other proteins are present in addition to these. Among total proteins, lectins are represented in both marine and freshwater microalgae, and have recently been also specifically identified in N. oleoabundans [ 60 , 61 , 62 ]. Interestingly, lectins have significant antiviral properties as preventive agents, which block interactions of viruses (or microbes in general) with the cell membrane since they occupy the sites for virus attachment to the cell membrane [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Neochloris oleoabundans under Different Cultivation Modes and First Results on Bioactivity of Its Extracts against HCoV-229E Virus

Baldisserotto

Gentili

Rizzo

et al. 2022

Plants

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Microalgae are proposed in several biotechnological fields because of their ability to produce biomass enriched in high-value compounds according to cultivation conditions. Regarding the health sector, an emerging area focuses on natural products exploitable against viruses. This work deals with the characterization of the green microalga Neochloris oleoabundans cultivated under autotrophic and mixotrophic conditions as a source of whole aqueous extracts, tested as antivirals against HCoV-229E (Coronaviridae family). Glucose was employed for mixotrophic cultures. Growth and maximum quantum yield of photosystem II were monitored for both cultivations. Algae extracts for antiviral tests were prepared using cultures harvested at the early stationary phase of growth. Biochemical and morphological analyses of algae indicated a different content of the most important classes of bioactive compounds with antiviral properties (lipids, exo-polysaccharides, and total phenolics, proteins and pigments). To clarify which phase of HCoV-229E infection on MRC-5 fibroblast cells was affected by N. oleoabundans extracts, four conditions were tested. Extracts gave excellent results, mainly against the first steps of virus infection. Notwithstanding the biochemical profile of algae/extracts deserves further investigation, the antiviral effect may have been mainly promoted by the combination of proteins/pigments/phenolics for the extract derived from autotrophic cultures and of proteins/acidic exo-polysaccharides/lipids in the case of mixotrophic ones.

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“…Due to the increased nature of ANFs, protein meals or peanut meals cannot be used in aquafeed [ 213 ]. Microalgae have been shown to contain a variety of ANFs, such as Tetradesmus obliquus, Kirchneriella lunaris , and Pseudokirchneriella subcapitata [ 214 , 215 ]. Previous studies indicate that the tannic acid levels of S. maxima (6.86 mg/g) and C. vulgaris (1.44 mg/g) were comparable [ 216 ].…”

Section: Nutrients Digestibilitymentioning

confidence: 99%

An overview of microalgae biomass as a sustainable aquaculture feed ingredient: food security and circular economy

2022

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Sustainable management of natural resources is critical to food security. The shrimp feed and fishery sector is expanding rapidly, necessitating the development of alternative sustainable components. Several factors necessitate the exploration of a new source of environmentally friendly and nutrient-rich fish feed ingredients. Microalgal biomass has the potential to support the growth of fish and shrimp aquaculture for global food security in the bio-economy. Algal biorefineries must valorize the whole crop to develop a viable microalgae-based economy. Microalgae have the potential to replace fish meal and fish oil in aquaculture and ensure sustainability standards. Microalgae biomasses provide essential amino acids, valuable triglycerides such as lipids, vitamins, and pigments, making them suitable as nutritional supplements in livestock feed formulations. Fish and microalgae have similar nutritional profiles, and digestibility is a critical aspect of the aquafeed formulation. A highly digestible feed reduces production costs, feed waste, and the risk of eutrophication. Due to low input costs, low carbon footprint, wastewater treatment benefits, and carbon credits from industrial CO 2 conversion, microalgae-based fish and shrimp feeds have the potential to provide significant economic benefits. However, several challenges must be addressed before microalgal biomass and bioproducts may be used as fish feeds, including heavy metal bioaccumulation, poor algal biomass digestion, and antinutrient effects. Knowledge of biochemical composition is limited and diverse, and information on nutritional value is scattered or contradictory. This review article presents alternative approaches that could be used in aquaculture to make microalgal biomass a viable alternative to fish meal.

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The green microalgae Tetradesmus obliquus (Scenedesmus acutus) as lectin source in the recognition of ABO blood type: purification and characterization

Cited by 20 publications

References 37 publications

A puriﬁed lectin with larvicidal activity from a woodland mushroom, Agaricus semotus Fr.

A puriﬁed lectin with larvicidal activity from a woodland mushroom, Agaricus semotus Fr.

Characterization of Neochloris oleoabundans under Different Cultivation Modes and First Results on Bioactivity of Its Extracts against HCoV-229E Virus

An overview of microalgae biomass as a sustainable aquaculture feed ingredient: food security and circular economy

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