Investigation of the major factors determining tropical upper-level cloud radiative effect (TUCRE) is crucial for understanding cloud feedback mechanisms. We examined the TUCRE inferred from the outputs of historical runs and AMIP runs from CMIP6 models employing a radiative-convective equilibrium (RCE). In this study, we incorporated the RCE model configurations of atmospheric dynamics and thermodynamics from the climate models, while simplifying the intricate systems. Using the RCE model, we adjusted the global mean surface temperature to achieve energy balance, considering variations in tropical cloud fraction, regional reflectivity, and emission temperature corresponding to each climate model. Subsequently, TUCRE was calculated as a unit of K/%, representing the change in global mean surface temperature (K) in response to an increment in the tropical upper-level clouds (%). Our RCE model simulation indicates that the major factors determining the TUCRE are the emission temperatures of tropical moist-cloudy and moist-clear regions, as well as the fraction of tropical upper-level clouds. The higher determination coefficients between TUCRE and both the emission temperature of tropical moist regions and the upper-level cloud fraction are attributable to their contribution to the trapping effect on the outgoing longwave radiations, which predominantly determines TUCRE. Consequently, the results of this study underscore the importance of accurately representing the upper-level cloud fraction and emission temperature in tropical moist regions to enhance the representation of TUCRE in climate models.