The Greening of Anthocyanins: Eco-Friendly Techniques for Their Recovery from Agri-Food By-Products

Lianza, Mariacaterina; Marincich, Lorenzo; Antognoni, Fabiana

doi:10.3390/antiox11112169

Cited by 20 publications

(10 citation statements)

References 100 publications

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“…The standard peaks of Del-delphinidin-3-mono glucoside (D3G) and Petunidin-3-mono glucoside (P3G) were observed at 3.1 and 5.1 min, respectively (S1 and S2). This phenomenon has been documented previously that characterized anthocyanin profiles of red table grape cultivars [ 48 ].…”

Section: Resultssupporting

confidence: 66%

“…This study further indicated that Delphinidin-3-glucoside was the primary anthocyanin that has been linked to numerous health benefits, such as antidiabetic and antiradical activities. Furthermore, Pomar, Novo and Masa [ 48 ] estimated a typical HPLC chromatogram of anthocyanin at 546 nm. The authors calculated the chemical structure of anthocyanins by hydrolysis and chromatographic analysis.…”

Section: Resultsmentioning

confidence: 99%

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Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color

Bakhsh

Cho

Baritugo

et al. 2023

Foods

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Color is a major feature that strongly influences the consumer’s perception, selection, and acceptance of various foods. An improved understanding regarding bio-safety protocols, health welfare, and the nutritional importance of food colorants has shifted the attention of the scientific community toward natural pigments to replace their toxic synthetic counterparts. However, owing to safety and toxicity concerns, incorporating natural colorants directly from viable sources into plant-based meat (PBM) has many limitations. Nonetheless, over time, safe and cheap extraction techniques have been developed to extract the purified form of coloring agents from raw materials to be incorporated into PBM products. Subsequently, extracted anthocyanin has displayed compounds like Delphinidin-3-mono glucoside (D3G) at 3.1 min and Petunidin-3-mono glucoside (P3G) at 5.1 277, 515, and 546 nm at chromatographic lambda. Fe-pheophytin was successfully generated from chlorophyll through the ion exchange method. Likewise, the optical density (OD) of synthesized leghemoglobin (LegH) indicated that pBHA bacteria grow more rigorously containing ampicillin with a dilution factor of 10 after 1 h of inoculation. The potential LegH sequence was identified at 2500 bp through gel electrophoresis. The color coordinates and absorbance level of natural pigments showed significant differences (p < 0.05) with the control. The development of coloring agents originating from natural sources for PBM can be considered advantageous compared to animal myoglobin in terms of health and functionality. Therefore, the purpose of this study was to produce natural coloring agents for PBM by extracting and developing chlorophyll from spinach, extracting anthocyanins from black beans, and inserting recombinant plasmids into microorganisms to produce LegH.

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Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color

Bakhsh

Cho

Baritugo

et al. 2023

Foods

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“…The extraction of specialized metabolites from plant material is influenced by several factors, such as the solvent type, the pH, the chemical stability of the target molecules, and specific operative parameters of the instrumentation. Temperature, time, and the solid-to-liquid ratio are among the most impacting parameters on polyphenol extraction [ 37 , 38 , 39 ]; hence, they were chosen as quantitative factors for the optimization of anthocyanin recovery from grape pomace through the DoE.…”

Section: Resultsmentioning

confidence: 99%

Green Method Comparison and Optimization of Anthocyanin Recovery from “Sangiovese” Grape Pomace: A Critical Evaluation of the Design of Experiments Approach

Lianza,

Antognoni

2024

Molecules

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Grape pomace is the main by-product obtained from wine production that is still enriched in bioactive compounds. Within a framework of waste/by-product reuse through a sustainable approach, various green methods were utilized in this work to recover anthocyanins from the pomace resulting from “Sangiovese” grape vinification. Ultrasound- and Microwave-Assisted Extractions (UAE and MAE) were coupled with the use of green solvents, such as acidified water, an ethanol/water mixture, and Natural Deep Eutectic Solvents (NaDES), and their efficacy was compared with that of a conventional method based on a methanol/acidified water mixture. The Total Anthocyanin Index ranged from 36.9 to 75.2 mg/g DW for UAE, and from 54.4 to 99.6 mg/g DW for MAE, while resulting in 47.1 mg/g DW for conventional extraction. A Design of Experiments (DoE) approach was applied to MAE, the most efficient technique. Temperature, time, and the solid-to-liquid ratio were set as X variables, while malvidin-3-O-glucoside content and antioxidant activity were used as response variables, measured by High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. The correlation between temperature and time and the antioxidant activity of the extract was positive, while it was found to be negative when considering malvidin-3-O-glucoside concentration as a response variable. Thus, the optimal conditions in temperature, time and solid-to-liquid ratio were different depending on the chosen variable. The results underline the importance of selecting an accurate response when using the response surface methodology approach.

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“…scCO 2 is a commonly used extractant in the pharmaceutical and natural products industries [69][70][71][72][73]. It is a non-toxic, non-flammable, and environmentally friendly solvent [74,75], and it can be easily removed by depressurization and evaporation [76][77][78]. The extracts were obtained with scCO 2 at 50 • C under 2000 (a) and 6000 PSI (b) (Table 3).…”

Section: Extractionmentioning

confidence: 99%

The Antioxidant and Neuroprotective Potential of Leaves and Inflorescences Extracts of Selected Hemp Varieties Obtained with scCO2

Stasiłowicz-Krzemień,

Sip,

Szulc

et al. 2023

Antioxidants

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Cannabis sativa, a versatile plant with numerous varieties, holds promising potential for a wide range of biological activity. As raw materials for research, we chose leaves and inflorescences of hemp varieties such as Białobrzeskie, Henola, and Tygra, which are cultivated mainly for their fibers or seeds. The choice of extraction is a key step in obtaining the selected compositions of active compounds from plant material. Bearing in mind the lipophilic nature of cannabinoids, we performed supercritical carbon dioxide (scCO2) extraction at 50 °C under 2000 (a) and 6000 PSI (b). The cannabinoid contents were determined with the use of the HPLC-DAD method. The antioxidant capabilities were assessed through a series of procedures, including the DPPH, ABTS, CUPRAC, and FRAP methods. The capacity to inhibit enzymes that play a role in the progression of neurodegenerative diseases, such as acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase was also studied. The dominant cannabinoids in the extracts were cannabidiol (CBD) and cannabidiolic acid (CBDA). The highest concentration of eight cannabinoids was detected in the Tygra inflorescences extract (b). The most notable antioxidant properties were provided by the Tygra inflorescences extract (b). Nonetheless, it was the Henola inflorescences extract (b) that demonstrated the most efficient inhibition of AChE and BChE, and tyrosinase was inhibited the most significantly by the Białobrzeskie inflorescences extract (b). Multidimensional comparative analysis enrolled all assays and revealed that the Henola inflorescences extract (b) showed the most substantial neuroprotective potential.

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The Greening of Anthocyanins: Eco-Friendly Techniques for Their Recovery from Agri-Food By-Products

Cited by 20 publications

References 100 publications

Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color

Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color

Green Method Comparison and Optimization of Anthocyanin Recovery from “Sangiovese” Grape Pomace: A Critical Evaluation of the Design of Experiments Approach

The Antioxidant and Neuroprotective Potential of Leaves and Inflorescences Extracts of Selected Hemp Varieties Obtained with scCO2

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