The GroES homolog of Helicobacter pylori confers protective immunity against mucosal infection in mice.

Abstract

Helicobacter pylori is an important etiologic agent of gastroduodenal disease. In common with other organisms, H. pylori bacteria express heat shock proteins that share homologies with the GroES-GroEL class of proteins from Escherichia coli. We have assessed the heat shock proteins of H. pylori as potential protective antigens in a murine model of gastric Helicobacter infection. Orogastric immunization of mice with recombinant H. pylori GroES-and GroELlike proteins protected 80% (n = 20) and 70% (n = 10) of animals, respectively, from a challenge dose of 104 Helicobacter felis bacteria (compared to control mice, P = 0.0042 and P = 0.0904, respectively). All mice (n = 19) that were immunized with a dual antigen preparation, consisting of H. pylori GroES-like protein and the B subunit ofH. pylori urease, were protected against infection. This represented a level of protection equivalent to that provided by a sonicated Helicobacter extract (P = 0.955). Antibodies directed against the recombinant H. pylori antigens were predominantly of the IgGl class, suggesting that a type 2 T-helper cell response was involved in protection. This work reports a protein belonging to the GroES class of heat shock proteins that was shown to induce protective immunity. In conclusion, GroES-like and urease B-subunit proteins have been identified as potential components of a future H. pylori subunit vaccine.

Helicobacter pylori Heat Shock Protein 60 Mediates Interleukin-6 Production by Macrophages via a Toll-like Receptor (TLR)-2-, TLR-4-, and Myeloid Differentiation Factor 88-independent Mechanism

“…600 g of H. pylori total soluble protein extracts were applied to an agarose column that had been coupled with 6 mg of a polyclonal antibody to H. pylori HSP60 (24) according to the manufacturer's instructions. The column was washed with PBS, and the flow-through was collected.…”

Role of Heat Shock Proteins in Diseases and Their Therapeutic Potential

“…Experimental infection of guinea pig with Chlamyida psittaci which causes trachoma revealed delayed type hypersensitive reaction against hsp60 [45]. Hsp60 have been shown to act as major antigen in diseases like pertuissis, gastritis caused by Helicobacter pylori, histoplasmosis, toxoplasmosis and lyme disease [46][47][48][49]. In onchocercosis caused by Onchocerca volvulus, hsp70 represent as major immunogen during infection [50].…”

Recombinant GroES in combination with CpG oligodeoxynucleotides protects mice against Mycobacterium avium infection

“…However, immunisation of mice with GroES and GroES-like proteins protected animals against M. leprae [10] and H. pylori infections [11], respectively. Furthermore, DNA vaccines encoding the groEL gene induced very strong antituberculous activity in mice [29].…”

“…For instance, intradermal immunisation of mice with recombinant M. leprae GroES, emulsified in Freund's incomplete adjuvant, has been shown to protect animals against multiplication of M. leprae [10]. Furthermore, mucosal immunisation with a recombinant Helicobacter pylori GroES-like protein and cholera toxin has been shown to protect mice against H. pylori infection [11].…”