The Growing Family of Plant Cyclin-Dependent Kinases with Multiple Functions in Cellular and Developmental Regulation

Dudits, Dénes; Cserháti, Mátyás; Miskolczi, Pál; Horváth, Gábor

doi:10.1002/9780470988923.ch1

Cited by 19 publications

(21 citation statements)

References 88 publications

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“…Cyclin‐dependent kinases (CDKs) are key regulators of the cell cycle, which need to interact with Cyclins (Cyc) for their activity. The A‐type CDKs form a major class of CDKs encoded by multiple (1–4) distinct genes in plants (Adachi et al ., ; Dudits et al ., ; Joubès et al ., ). In plants, they are constitutively expressed throughout the cell cycle and play roles during S‐ and M‐phase progression (Joubès et al ., ).…”

Section: Introductionmentioning

confidence: 98%

The cell size distribution of tomato fruit can be changed by overexpression of CDKA1

Czerednik

Busscher

Angenent

et al. 2014

Plant Biotechnology Journal

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SummaryTomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing industry). To improve the quality of tomato, it is important to understand the regulation of fruit development and of fruit cellular structure, which is in part determined by the sizes and numbers of cells within a tissue. The role of the cell cycle therein is poorly understood. Plant cyclin-dependent kinases (CDKs) are homologues of yeast cdc2, an important cell cycle regulator conserved throughout all eukaryotes. CDKA1 is constitutively expressed during the cell cycle and has dual functions in S-and M-phase progression. We have produced transgenic tomato plants with increased expression of CDKA1 under the control of the fruit-specific TPRP promoter, which despite a reduced number of seeds and diminished amount of jelly, developed fruits with weight and shape comparable to that of wild-type fruits. However, the phenotypic changes with regard to the pericarp thickness and placenta area were remarkable. Fruits of tomato plants with the highest expression of CDKA1 had larger septa and columella (placenta), compared with wild-type fruits. Our data demonstrate the possibility of manipulating the ratio between cell division and expansion by changing the expression of a key cell cycle regulator and probably its activity with substantial effects on structural traits of the harvested fruit.

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Section: Introductionmentioning

confidence: 98%

The cell size distribution of tomato fruit can be changed by overexpression of CDKA1

Czerednik

Busscher

Angenent

et al. 2014

Plant Biotechnology Journal

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“…In plants, >160 CDK-related genes have been cloned from >20 higher plant species and are apportioned into classes A–G and CDK-like (Dudits et al ., 2007). The CDKB family is unique to plants.…”

Section: Introductionmentioning

confidence: 99%

Plant WEE1 kinase is cell cycle regulated and removed at mitosis via the 26S proteasome machinery

Cook

Gronlund

Siciliano

et al. 2013

Journal of Experimental Botany

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In yeasts and animals, premature entry into mitosis is prevented by the inhibitory phosphorylation of cyclin-dependent kinase (CDK) by WEE1 kinase, and, at mitosis, WEE1 protein is removed through the action of the 26S proteasome. Although in higher plants WEE1 function has been confirmed in the DNA replication checkpoint, Arabidopsis wee1 insertion mutants grow normally, and a role for the protein in the G2/M transition during an unperturbed plant cell cycle is yet to be confirmed. Here data are presented showing that the inhibitory effect of WEE1 on CDK activity in tobacco BY-2 cell cultures is cell cycle regulated independently of the DNA replication checkpoint: it is high during S-phase but drops as cells traverse G2 and enter mitosis. To investigate this mechanism further, a yeast two-hybrid screen was undertaken to identify proteins interacting with Arabidopsis WEE1. Three F-box proteins and a subunit of the proteasome complex were identified, and bimolecular fluorescence complementation confirmed an interaction between AtWEE1 and the F-box protein SKP1 INTERACTING PARTNER 1 (SKIP1). Furthermore, the AtWEE1–green fluorescent protein (GFP) signal in Arabidopsis primary roots treated with the proteasome inhibitor MG132 was significantly increased compared with mock-treated controls. Expression of AtWEE1–YFPC (C-terminal portion of yellow fluorescent protein) or AtWEE1 per se in tobacco BY-2 cells resulted in a premature increase in the mitotic index compared with controls, whereas co-expression of AtSKIP1–YFPN negated this effect. These data support a role for WEE1 in a normal plant cell cycle and its removal at mitosis via the 26S proteasome.

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“…They act as serine-threonine kinases in complexes together with the regulatory cyclin subunit ( Van Leene et al , 2010 ). Five families of CDKs (A–F) are known in plants, of which CDKA and CDKB are the most prominent and numerous classes ( Joubès et al , 2000 ; Dudits et al , 2007 ). CDKA genes are widely present in different organisms and form the largest class of CDK genes ( Dudits et al , 2007 ).…”

Section: Introductionmentioning

confidence: 99%

“…Five families of CDKs (A–F) are known in plants, of which CDKA and CDKB are the most prominent and numerous classes ( Joubès et al , 2000 ; Dudits et al , 2007 ). CDKA genes are widely present in different organisms and form the largest class of CDK genes ( Dudits et al , 2007 ). These proteins are characterized by the presence of the PSTAIRE motif, which is essential for cyclin binding ( Joubès et al , 1999 ).…”

Section: Introductionmentioning

confidence: 99%

Regulation of tomato fruit pericarp development by an interplay between CDKB and CDKA1 cell cycle genes

Czerednik

Busscher

Bielen

et al. 2012

Journal of Experimental Botany

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Growth of tomato fruits is determined by cell division and cell expansion, which are tightly controlled by factors that drive the core cell cycle. The cyclin-dependent kinases (CDKs) and their interacting partners, the cyclins, play a key role in the progression of the cell cycle. In this study the role of CDKA1, CDKB1, and CDKB2 in fruit development was characterized by fruit-specific overexpression and down-regulation. CDKA1 is expressed in the pericarp throughout development, but is strongly up-regulated in the outer pericarp cell layers at the end of the growth period, when CDKB gene expression has ceased. Overexpression of the CDKB genes at later stages of development and the down-regulation of CDKA1 result in a very similar fruit phenotype, showing a reduction in the number of cell layers in the pericarp and alterations in the desiccation of the fruits. Expression studies revealed that CDKA1 is down-regulated by the expression of CDKB1/2 in CDKB1 and CDKB2 overexpression mutants, suggesting opposite roles for these types of CDK proteins in tomato pericarp development.

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The Growing Family of Plant Cyclin-Dependent Kinases with Multiple Functions in Cellular and Developmental Regulation

Cited by 19 publications

References 88 publications

The cell size distribution of tomato fruit can be changed by overexpression of CDKA1

The cell size distribution of tomato fruit can be changed by overexpression of CDKA1

Plant WEE1 kinase is cell cycle regulated and removed at mitosis via the 26S proteasome machinery

Regulation of tomato fruit pericarp development by an interplay between CDKB and CDKA1 cell cycle genes

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