The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation

Raj, A. Thirumal; Kheur, Supriya; Khurshid, Zohaib; Sayed, Mohammed E.; Mugri, Maryam H.; Almasri, Mazen; Al-Ahmari, Manea Musa; Patil, Vikrant R; Bhandi, Shilpa; Testarelli, Luca; Patil, Shankargouda

doi:10.3390/molecules26185683

Cited by 13 publications

(9 citation statements)

References 20 publications

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“…Dominant therapeutic effects of DPSCs depend on the presence of growth factors, cytokines and osteoinductive agents. Possibly, there are several uninvestigated approaches through the modulation of growth factors and cytokine productions 15 . For example, researchers' DPSC treatments with cobalt-chromium and hypoxic induction have led to growth factor alterations in the cells, enhancing their regenerative potentials 14 .…”

Section: Discussionmentioning

confidence: 99%

Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned Media of Probiotics Bacteria

Amini¹,

Rezvani²,

Bakhtiari³

et al. 2023

AJMB

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Background: Stem cells are used to treat numerous diseases; however, their lifespan is rather short. Factors such as probiotics affect and improve various cell lineage efficacies. The aim of this study was to investigate the effects of probiotics-conditioned media on dental pulp stem cell potentials in osteogenesis. Methods: The experiment was initiated by culturing Lactobacillus casei and Lactobacillus acidophilus probiotics as well as DPS-7 cells. Bacterial supernatants were separated and concentrated as the conditioned media. The DPS-7 cells were treated with various concentrations of the conditioned media. Furthermore, MTT assay and alkaline phosphatase activity were used. The mRNA expression of three genes (bFGF, EGF-β and BMP-2) involved in osteogenesis was analyzed using a real-time polymerase chain reaction. Results: The response of dental pulp stem cells to probiotics preconditioning promoted cell proliferation, increased alkaline phosphatase activity and upregulated bFGF and BMP-2 gene expression. Increased expression was significant for BMP-2 and moderate for bFGF; however, it was non-significant for EGF-β. The use of the two probiotics was the most effective. Conclusion: In general, synergism of the combined probiotics preconditioning induces differentiation of DPS-7 cells into osteoblasts most effectively.

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Section: Discussionmentioning

confidence: 99%

Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned Media of Probiotics Bacteria

Amini¹,

Rezvani²,

Bakhtiari³

et al. 2023

AJMB

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“…TNF-a is an inflammatory cytokine which takes part in the maintenance of various cellular signaling processes (118). TNF-a is one of the most abundant pro-inflammatory cytokines followed by CXCL-8 in the oral MSC secretome, which could potentially mediate tumor development (119).…”

Section: Tumor Necrosis Factor Alphamentioning

confidence: 99%

“…TNF-α is an inflammatory cytokine which takes part in the maintenance of various cellular signaling processes ( 118 ). TNF-α is one of the most abundant pro-inflammatory cytokines followed by CXCL-8 in the oral MSC secretome, which could potentially mediate tumor development ( 119 ). Tumor associated macrophages (TAMs) play a vital role in the production of TNF-α which can be associated with increased stemness in OC ( 120 ).…”

Section: Extrinsic Factors Governing Oscc Stemnessmentioning

confidence: 99%

“…Immune dysregulation in the TME of this sort could potentially favor malignant progression, recurrence of tumors, and poor patient prognosis ( 126 ). CXCL8 is another pro-inflammatory cytokine that was found to be a vital component in the propagation of Oral Cancer Cells (OCC) ( 119 ). CCL2 is a major player in proliferation, migration, invasion, and tumor growth, via phosphorylation of NF-κB and STAT3 pathways.…”

Section: Extrinsic Factors Governing Oscc Stemnessmentioning

confidence: 99%

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Panoramic view of key cross-talks underpinning the oral squamous cell carcinoma stemness - unearthing the future opportunities

Vastrad,

Ritesh,

et al. 2023

Front. Oncol.

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The clinical management of oral cancer is often frequented with challenges that arise from relapse, recurrence, invasion and resistance towards the cornerstone chemo and radiation therapies. The recent conceptual advancement in oncology has substantiated the role of cancer stem cells (CSC) as a predominant player of these intricacies. CSC are a sub-group of tumor population with inherent adroitness to self-renew with high plasticity. During tumor evolution, the structural and functional reprogramming persuades the cancer cells to acquire stem-cell like properties, thus presenting them with higher survival abilities and treatment resistance. An appraisal on key features that govern the stemness is of prime importance to confront the current challenges encountered in oral cancer. The nurturing niche of CSC for maintaining its stemness characteristics is thought to be modulated by complex multi-layered components encompassing neoplastic cells, extracellular matrix, acellular components, circulatory vessels, various cascading signaling molecules and stromal cells. This review focuses on recapitulating both intrinsic and extrinsic mechanisms that impart the stemness. There are contemplating evidences that demonstrate the role of transcription factors (TF) in sustaining the neoplastic stem cell’s pluripotency and plasticity alongside the miRNA in regulation of crucial genes involved in the transformation of normal oral mucosa to malignancy. This review illustrates the interplay between miRNA and various known TF of oral cancer such as c-Myc, SOX, STAT, NANOG and OCT in orchestrating the stemness and resistance features. Further, the cross-talks involved in tumor micro-environment inclusive of cytokines, macrophages, extra cellular matrix, angiogenesis leading pathways and influential factors of hypoxia on tumorigenesis and CSC survival have been elucidated. Finally, external factorial influence of oral microbiome gained due to the dysbiosis is also emphasized. There are growing confirmations of the possible roles of microbiomes in the progression of oral cancer. Given this, an attempt has been made to explore the potential links including EMT and signaling pathways towards resistance and stemness. This review provides a spectrum of understanding on stemness and progression of oral cancers at various regulatory levels along with their current therapeutic knowledge. These mechanisms could be exploited for future research to expand potential treatment strategies.

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“…Based on Ki-67 assays, Raj et al (2021b) indicated the dual effect of MSCs on tumors. DPSCs-CM inhibited the proliferation of TSCC cells AW13516 at 50% and 100% concentrations and promoted the proliferation at a 20% concentration.…”

Section: Effects Of Mesenchymal Stem Cells On Oral Cancermentioning

confidence: 99%

The Effects of Mesenchymal Stem Cells on Oral Cancer and Possible Therapy Regime

et al. 2022

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Mesenchymal stem cells (MSCs) are characterized by self-renewal, rapid proliferation, multipotent differentiation, and low immunogenicity. In addition, the tropism of MSCs towards injured tissues and tumor lesions makes them attractive candidates as cell carriers for therapeutic agent delivery and genetic material transfer. The interaction between tumor cells and MSCs in the tumor microenvironment plays an important role in tumor progression. Oral cancer is one of the most common malignant diseases in the head and neck. Although considerable improvements in the treatment of oral cancer were achieved, more effective and safer novel agents and treatments are still needed, and deeper studies on the etiology, pathology, and treatment of the oral cancer are desirable. In the past decades, many studies have reported the beneficial effects of MSCs-based therapies in the treatment of various diseases, including oral cancers. Meanwhile, other studies demonstrated that MSCs may enhance the growth and metastasis of oral cancer. In this paper, we reviewed the research progress of the effects of MSCs on oral cancers, the underlying mechanisms, and their potential applications in the treatment of oral cancers.

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The Growth Factors and Cytokines of Dental Pulp Mesenchymal Stem Cell Secretome May Potentially Aid in Oral Cancer Proliferation

Cited by 13 publications

References 20 publications

Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned Media of Probiotics Bacteria

Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned Media of Probiotics Bacteria

Panoramic view of key cross-talks underpinning the oral squamous cell carcinoma stemness - unearthing the future opportunities

The Effects of Mesenchymal Stem Cells on Oral Cancer and Possible Therapy Regime

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