The growth of single roots ofArtemisia annua in nutrient mist reactors

Wyslouzil, Barbara E.; Waterbury, Raymond G.; Weathers, Pamela J.

doi:10.1002/1097-0290(20001020)70:2<143::aid-bit3>3.0.co;2-b

Cited by 37 publications

(30 citation statements)

References 19 publications

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“…This problem was overcome by growing hairy roots in gas-phase reactors like Mist reactor. In nutrient mist bioreactor, the nutrient was sprayed in the form of fine nano-sized droplets Wyslouzil et al 2000). The reactor consists of a mist generator (ultrasonic, acoustic or nozzle type), nutrient reservoir and a culture chamber.…”

Section: Resultsmentioning

confidence: 99%

“…However, increasing the misting cycle beyond a certain limit also affects growth negatively as mass transfer resistant thin-film deposits over the root surface during 'mist duty ON' period ). Other factors that affect growth of roots in a nutrient mist bioreactor are mist flow direction, inoculum distribution, gas composition, medium concentration Wyslouzil et al 1997Wyslouzil et al , 2000, etc. It has been advocated that conditioned medium has been much better than fresh medium for growth of hairy roots in mist bioreactor cultivation (Wyslouzil et al 2000) as roots exude useful chemicals like oligosaccharides, auxins and peptides.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

Patra

Srivastava

2015

Plant Cell Rep

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Alternative biotechnological protocol for large-scale artemisinin production was established. It featured enhanced growth and artemisinin production by cultivation of hairy roots in nutrient mist bioreactor (NMB) coupled with novel cultivation strategies. Artemisinin is used for the treatment of cerebral malaria. Presently, its main source is from seasonal plant Artemisia annua. This study featured investigation of growth and artemisinin production by A. annua hairy roots (induced by Agrobacterium rhizogenes-mediated genetic transformation of explants) in three bioreactor configurations-bubble column reactor, NMB and modified NMB particularly to establish their suitability for commercial production. It was observed that cultivation of hairy roots in a non-stirred bubble column reactor exhibited a biomass accumulation of 5.68 g/l only while batch cultivation in a custom-made NMB exhibited a higher biomass concentration of 8.52 g/l but relatively lower artemisinin accumulation of 0.22 mg/g was observed in this reactor. A mixture of submerged liquid-phase growth (for 5 days) followed by gas-phase cultivation in nutrient mist reactor operation strategy (for next 15 days) was adopted for hairy root cultivation in this investigation. Reasonably, high (23.02 g/l) final dry weight along with the artemisinin accumulation (1.12 mg/g, equivalent to 25.78 mg/l artemisinin) was obtained in this bioreactor, which is the highest reported artemisinin yield in the gas-phase NMB cultivation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Artemisinin production by plant hairy root cultures in gas- and liquid-phase bioreactors

Patra

Srivastava

2015

Plant Cell Rep

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“…Also, the hairy roots showed plagiogeotropism (lack of geotropism) in liquid medium as expected (Figure 3). In this period, roots propagated by three means: meristematic cell propagation at the tip, cell elongation, and branching via side root production (33).…”

Section: Effect Of Agrobacterium Rhizogenes Strains and Cocultivationmentioning

confidence: 99%

Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

Jaberi¹,

Sharafi²,

Sharafi³

et al. 2018

Jundishapur J Nat Pharm Prod

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Background: Cosmos bipinnatus is an important medicinal plant with antioxidative, antigenotoxic, anti-inflammatory, and antiproliferative effects on several cancer cell lines. It has great potential for development as a promising cancer chemo-preventive agent. Hairy root-based culture technology is a new sustainable production platform for producing specific pharmaceutical secondary metabolites. Objectives: The current study developed and introduced a reliable transformation system for C. bipinnatus by optimization of aspects important in transformation frequency using Agrobacterium rhizogenes. Methods: Five bacterial strains, including ATCC 15834, ATCC 31798, A7, MAFF-02-10266, and MSU440, 2 explant types (leaf and stem), and 2 co-cultivation media (full MS and ½ MS) were examined. Genomic DNA was extracted using a modified CTAB protocol from putative transgenic root lines and the control root. Transgenic hairy root lines were approved by means of Polymerase Chain Reaction (PCR) using specific rolB gene primers. Results:The highest ratio of genetically transformed root induction was found from leaf explants using A. rhisogenes strains ATCC15834 and MSU440 (72% to 73%). When ½ MS medium was used as a co-cultivation medium, a significant increase in transformation frequency (84%) was observed. Conclusions: The MSU440 Agrobacterium strain and ½ MS co-cultivation medium could significantly improve genetic transformation efficiency for establishment of hairy root-based cultures for C. bipinnatus.

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“…In many of the studies to date, the nutrient media used in the experiments have been ''conditioned.'' Conditioned medium is produced by growing roots in shake flasks containing fresh, autoclaved media for up to 2 weeks (14). The roots adjust the distribution of sugars between sucrose, glucose, and fructose, the levels of salts, and exude other compounds, for example, auxins (15), that have a positive effect on root growth.…”

Section: Nutrient Mist Reactor Design and Operationmentioning

confidence: 99%