The growth of tumours in T-cell deprived mice and their response to treatment with<i>Corynebacterium parvum</i>

Woodruff, M. F. A.; Dunbar, Noreen; Ghaffar, Abdul

doi:10.1098/rspb.1973.0034

Cited by 71 publications

(24 citation statements)

References 3 publications

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“…However, as C. parvum is a known immunopotentiating agent (Halpern et al, 1963;Howard, Scott and Christie, 1973), most emphasis has been on the role played by macrophages and T cells (Woodruff, Dunbar and Ghaffar, 1973;Olivotto and Bomford, 1974). In this paper we present evidence of a non-immunological action of C. parrum which could possibly inhibit tumours, particularly metastases, in the liver and lungs.…”

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confidence: 59%

Intravascular coagulation resulting from intravenous injection of C. parvum in mice

Lampert¹,

Jones²,

Sadler³

et al. 1977

Br J Cancer

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In mice, i.v. C. parvum induces intravascular coagulation. This is a prolonged reaction lasting up to 7 days. It results in thrombosis in hepatic vessels with consequent hepatic necrosis, and thrombosis in pulmonary and splenic vessels. This may be important in the assessment of the tumour-inhibitory activity of C. parvum. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4

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mentioning

confidence: 59%

Intravascular coagulation resulting from intravenous injection of C. parvum in mice

Lampert¹,

Jones²,

Sadler³

et al. 1977

Br J Cancer

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“…Mice.-The recipient mice were either intact adult CBA females (20-22 g) or T-cell deprived CBA females prepared as described previously (Woodruff et al, 1973).…”

Section: Methodsmentioning

confidence: 99%

“…We have used as tumour hosts both intact and T-cell deprived mice, because in our own experience (Wooodruff and Dunbar, 1972;Woodruff, Dunbar and Ghaffar, 1973), the anti-tumour effect of systemic injections of active strains of C. parvum in respect of cholanthrene induced sarcomata is maintained in T-cell deficient mice, whereas Scott (1974) has reported that the growth of a mastocytoma, is inhibited by intratumour injection of C. parvum in intact mice but not in T-cell deprived mice.…”

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Effect of local injection of Corynebacterium parvum on the growth of a murine fibrosarcoma

Woodruff¹,

Dunbar²

1975

Br J Cancer

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Summary.-Local injection (i.e. injection at the site of tumour inoculation) of strains of C. Parvum which have a significant anti-tumour effect when given systemically (i.e. intravenously or, in the case of subcutaneous tumour transplant, intraperitoneally) strongly inhibits the growth of isogeneic transplants of a fibrosarcoma in intact CBA mice but has little or no effect on subcutaneous transplants of the same tumour in T-cell deprived mice. The anti-tumour effect of local injection of C. parvum, unlike that of systemic injection in this particular tumour system, thus appears to be T-cell dependent.PRELIMINARY observations of our own with a mouse fibrosarcoma, and experiments with other mouse tumours recently reported by Likhite and Halpern (1974) and Scott (1974), have shown that intratumour injection of C. parvum may strongly inhibit tumour growth and under some conditions cause complete regression.The present experiments were designed to investigate this phenomenon further, using both subcutaneous and intraperitoneal tumour transplants. To exclude purely mechanical effects we have compared the degree of tumour inhibition resulting from local injection of 3 anaerobic coryneforms which differ markedly in their effect on tumour growth when given systemically, and of an aerobic organism, C. diphtheriae.We have used as tumour hosts both intact and T-cell deprived mice, because in our own experience (Wooodruff and Dunbar, 1972;Woodruff, Dunbar and Ghaffar, 1973), the anti-tumour effect of systemic injections of active strains of C. parvum in respect of cholanthrene induced sarcomata is maintained in T-cell deficient mice, whereas Scott (1974) has reported that the growth of a mastocytoma, is inhibited by intratumour injection of C. parvum in intact mice but not in T-cell deprived mice. MATERIALS AND METHODSMice.-The recipient mice were either intact adult CBA females (20-22 g) or T-cell deprived CBA females prepared as described previously (Woodruff et al., 1973).Tumour. The tumour was originally induced in a female CBA mouse with methylcholanthrene. It was stored in liquid nitrogen after 1]5 transplant generations and was transplanted once more before being used in the experiments. The properties of this tumour have been summarized in a recent review (Woodruff, 1975). It was transplanted subcutaneously (right foreleg) or intraperitoneally, or by both these routes, in the form of a cell suspension prepared with pronase in a dosage (unless otherwise stated) of 104 cells.The mice were inspected and weighed every 2 days. If a subcutaneous tumour was present, its width in 2 directions at rightangles was measured with a caliper and the mean was recorded. Where appropriate, the diameters of subcutaneous tumours were summed, as described previously (Woodruff et al., 1973), up to the day when some of the tumours in control mice were 18-20 mm in diameter, and the group mean sums were compared by Student's t-test. In many cases, however, the differences between groups were sufficiently clearcut to make this unnecessary.

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“…The present experiments also establish that the increase in certain Ig levels (and possibly anti-tumourresponses) following C. parvum administration are to some extent independent of T-cell function. These serological observations are important in relation to the known antitumour properties of systemically administered C. parvum in T-deprived and nude mice (Woodruff et al, 1973;Woodruff and Warner, 1977).…”

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confidence: 99%

Serological changes associated with C. parvum treatment in nude mice

James¹,

Woodruff²,

McBride³

et al. 1977

Br J Cancer

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The growth of tumours in T-cell deprived mice and their response to treatment withCorynebacterium parvum

Cited by 71 publications

References 3 publications

Intravascular coagulation resulting from intravenous injection of C. parvum in mice

Intravascular coagulation resulting from intravenous injection of C. parvum in mice

Effect of local injection of Corynebacterium parvum on the growth of a murine fibrosarcoma

Serological changes associated with C. parvum treatment in nude mice

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