The GSTM1 and GSTT1 Null Genotypes Increase the Risk for Type 2 Diabetes Mellitus and the Subsequent Development of Diabetic Complications: A Meta-analysis

Nath, Sayantan; Das, Sambuddha; Bhowmik, Aditi; Ghosh, Sankar Kumar; Choudhury, Yashmin

doi:10.2174/1573399814666171215120228

“…Such discrepancies may result from small sample sizes, race and methodology. Considering the controversial results in this regard, a few meta-analyses were performed, and they revealed that both GSTT1 and GSTM1 conferred increased risk for T2DM [38,39]. Our study con rmed that the frequency of null GSTT1 was higher in T2DM patients than in controls, indicating an association of the GSTT1 null genotype and the risk of T2DM in the Chinese population.…”

Section: Discussionsupporting

confidence: 62%

Influence of GSTT1 and GSTP1 Polymorphisms on Type 2 Diabetes Mellitus and Diabetic Retinopathy Risk in The Chinese Population: A Case Control Study

Geng

¹

,

Zha²,

Zhou

³

et al. 2020

Preprint

0

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Background: Studies have revealed the association of glutathione S-transferases (GSTM1 and GSTT1) deletion (null) polymorphism with the risks of developing type 2 diabetes mellitus (T2DM) and its complications. The present study aimed to investigate the relationship between GSTT1/ GSTP1 gene polymorphisms and the risks of T2DM and diabetic retinopathy (DR) in a Chinese population.Methods: A total of 336 subjects with T2DM and a defined ophthalmologic status were recruited from the Second People’s Hospital of Yunnan Province between June 2014 and October 2016. Seventy-two age-matched healthy controls were also enrolled. Physical examinations and laboratory tests were performed. The frequencies of GSTT1 and GSTP1 genotypes in all participants were determined by PCR and PCR-restriction fragment length polymorphisms (PCR–RFLP), respectively.Results: Compared with healthy controls, the GSTT1-null genotype was significantly more common in diabetic patients with or without DR (all P < 0.05). However, the frequency of the GSTP1 genotype (AA, GA, GG) was comparable between the two groups. Furthermore, neither the GSTP1 nor GSTT1 genetic polymorphism was associated with the development of DR. In the present study, the risk of developing T2DM was significantly higher in subjects carrying the combined heterozygous GSTP1 (AG) and null GSTT1 genotypes (OR=0.40, 95% CI=0.21-0.74, P=0.02).Conclusions: The deletion of the GSTT1 genotype was associated with a higher risk of developing T2DM, whether alone or in combination with GSTP1, indicating that the null genotype of GSTT1 may serve as a potential biomarker for T2DM in the Chinese population, which is helpful for clinicians to make more effective risk-based decisions.

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“…Such discrepancies may result from small sample sizes, race and methodology. Considering the controversial results in this regard, a few meta-analyses were performed, and they revealed that both GSTT1 and GSTM1 conferred increased risk for T2DM [38,39]. The present study rstly con rmed that the frequency of null GSTT1 was higher in T2DM patients than in controls, indicating an association of the GSTT1 null genotype and the risk of T2DM in the Chinese population.…”

Section: Discussionsupporting

confidence: 60%

“…The present study rstly con rmed that the frequency of null GSTT1 was higher in T2DM patients than in controls, indicating an association of the GSTT1 null genotype and the risk of T2DM in the Chinese population. The GSTT1-null allele has been reported to be associated with both macrovascular and microvascular disease in T2DM patients, including diabetic nephropathy and retinopathy [20,26,39,40,41]. In recent studies, it was demonstrated that the deletion polymorphism of GSTT1 could increase the risk for diabetic retinopathy in the Caucasian population [28,42].…”

Section: Discussionmentioning

confidence: 99%

Influence of GSTT1 and GSTP1 Polymorphisms on Type 2 Diabetes Mellitus and Diabetic Retinopathy Risk in The Chinese Population: A Case Control Study

Geng

¹

,

Zha²,

Zhou

³

et al. 2020

Preprint

1

0

View full text Add to dashboard Cite

Background: Studies have revealed the association of glutathione S-transferases (GSTM1 and GSTT1) deletion (null) polymorphism with the risks of developing type 2 diabetes mellitus (T2DM) and its complications. The present study aimed to investigate the relationship between GSTT1/ GSTP1 gene polymorphisms and the risks of T2DM and diabetic retinopathy (DR) in a Chinese population.Methods: A total of 336 subjects with T2DM and a defined ophthalmologic status were recruited from the Second People’s Hospital of Yunnan Province between June 2014 and October 2016. Seventy-two age-matched healthy controls were also enrolled. Physical examinations and laboratory tests were performed. The frequencies of GSTT1 and GSTP1 genotypes in all participants were determined by PCR and PCR-restriction fragment length polymorphisms (PCR–RFLP), respectively.Results: Compared with healthy controls, the GSTT1-null genotype was significantly more common in diabetic patients with or without DR (all P < 0.05). However, the frequency of the GSTP1 genotype (AA, GA, GG) was comparable between the two groups. Furthermore, neither the GSTP1 nor GSTT1 genetic polymorphism was associated with the development of DR. In the present study, the risk of developing T2DM was significantly higher in subjects carrying the combined heterozygous GSTP1 (AG) and null GSTT1 genotypes (OR=0.40, 95% CI=0.21-0.74, P=0.02).Conclusions: The deletion of the GSTT1 genotype was associated with a higher risk of developing T2DM, whether alone or in combination with GSTP1, indicating that the null genotype of GSTT1 may serve as a potential biomarker for T2DM in the Chinese population, which is helpful for clinicians to make more effective risk-based decisions.

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“…An increase in risk was observed regardless of ethnicity. 21 For all three genes analyzed, it was noted that as the number of gene combinations increases, there is a rise in the disease risk, especially in the subjects younger than 40, which suggest that a gene-gene interaction plays an important role in T2D susceptibility. Statistically significant differences were demonstrated for the combination of GSTP1 Val/Val and GSTT1null/null genotypes between patients diagnosed before 40 years of age and healthy people (12.5% in group A vs 0.9% in group C, p=0.016; table 4), also for the combination of all three genes: GSTP1 Val/Val, GSTT1null/null and GSTM1 null/ null (12.5% in group A vs 0.5% in group C, p=0.013; table 4).…”

Section: Discussionmentioning

confidence: 98%

Glutathione S-transferases gene polymorphism influence on the age of diabetes type 2 onset

Klusek

¹

,

Błońska-Sikora

²

,

Witczak

³

et al. 2020

BMJ Open Diab Res Care

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IntroductionType 2 diabetes (T2D) is a multifactorial disease affecting mostly adults older than 40 years. The aim of the study was to examine GST gene polymorphism influence on the risk of T2D, especially in young adults.Research design and methods200 diabetic patients and 221 healthy controls participated in this study. Three GST gene polymorphism have been analyzed: GSTP1 (single-nucleotide polymorphism Ile105Val), homozygous deletion of GSTT1 (null/null) and GSTM1 (null/null), using TaqMan real-time quantitative PCR.ResultsThe distribution of examined polymorphisms was similar in patient group and control group. Statistically significant differences were demonstrated for the combination of GSTP1 Val/Val and GSTT1 null/null genotypes between patients diagnosed before 40 years of age and healthy people (12.5% vs 0.9%, p=0.016). Moreover, all three examined gene polymorphism together (GSTP1 Val/Val, GSTM1nul/null and GSTT1 null/null genotype) was observed in 12.5% of patients diagnosed before 40 years of age and in 0.5% of healthy individuals (p=0.013).ConclusionIn conclusion, the results suggest that GST polymorphism may be one of the risk factors for developing T2D at a younger age than the T2D population average.

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The GSTM1 and GSTT1 Null Genotypes Increase the Risk for Type 2 Diabetes Mellitus and the Subsequent Development of Diabetic Complications: A Meta-analysis

Abstract: Thus GSTM1 and GSTT1 null genotypes increases the risk for Type 2 diabetes mellitus alone, in combination or with regards to ethnicity.

Cited by 21 publications

References 45 publications

Influence of GSTT1 and GSTP1 Polymorphisms on Type 2 Diabetes Mellitus and Diabetic Retinopathy Risk in The Chinese Population: A Case Control Study

Influence of GSTT1 and GSTP1 Polymorphisms on Type 2 Diabetes Mellitus and Diabetic Retinopathy Risk in The Chinese Population: A Case Control Study

Influence of GSTT1 and GSTP1 Polymorphisms on Type 2 Diabetes Mellitus and Diabetic Retinopathy Risk in The Chinese Population: A Case Control Study

Glutathione S-transferases gene polymorphism influence on the age of diabetes type 2 onset

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