The guanine nucleotide-binding protein α subunit protein ChGnaq positively regulates Hsc70 transcription in Crassostrea hongkongensis

Zhang, Lingli; Xu, Delin; Cui, Miao; Tang, Lei; Hou, Ting-Long; Zhang, Qizhong

doi:10.1016/j.bbrc.2018.03.130

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…Specific primers and the housekeeping gene EF-1α (elongation factor 1, alpha) are displayed in Supplementary Table S1 . The qRT-PCR was performed as previously described ( Zhang et al, 2018 ). Relative expression was evaluated by the 2 -ΔΔC T method ( Livak and Schmittgen, 2001 ).…”

Section: Materials and Methodssmentioning

confidence: 99%

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

et al. 2018

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The nuclear peroxisome proliferator-activated receptors (PPARs) regulate the transcription of elongases of very long-chain fatty acids (Elovl), which are involved in polyunsaturated fatty acid (PUFA) biosynthesis in mammals. In the present study, we first characterized the function of Elovl5 elongase in Trachinotus ovatus. The functional study showed that ToElovl5 displayed high elongation activity toward C18 and C20 PUFA. To investigate whether PPARαb was a regulator of Elovl5, we also reported the sequence of T. ovatus PPARαb (ToPPARαb). The open reading frame (ORF) sequence encoded 469 amino acids possessing four typical characteristic domains, including an N-terminal hypervariable region, a DNA-binding domain (DBD), a flexible hinge domain and a ligand-binding domain (LBD). Thirdly, promoter activity experiments showed that the region from PGL3-basic-Elovl5-5 (-146 bp to +459 bp) was defined as the core promoter by progressive deletion mutation of Elovl5. Moreover, PPARαb overexpression led to a clear time-dependent enhancement of ToElovl5 promoter expression in HEK 293T cells. Fourth, the agonist of PPARαb prominently increased PPARαb and Elovl5 expression, while PPARαb depletion by RNAi or an inhibitor was correlated with a significant reduction of Elovl5 transcription in T. ovatus caudal fin cells (TOCF). In conclusion, the present study provides the first evidence of the positive regulation of Elovl5 transcription by PPARαb and contributes to a better understanding of the transcriptional mechanism of PPARαb in fish.

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Section: Materials and Methodssmentioning

confidence: 99%

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

et al. 2018

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“…It is likely that ToNF-κB is an important transcription factor (He et al, 2021). ToNF-κB expression was highest in the intestine, suggesting that the intestine is an important tissue associated with the immune defence response to various stresses in pompanos (Zhang et al, 2018;He et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…The specific primers for ToNF-κB and ToTNFα and the housekeeping gene EF-1α (elongation factor 1, alpha) are shown in Table 1. The qRT-PCR procedure was executed as previously described (Zhang et al, 2018). To evaluate the relative expression of those genes, the 2 -ΔΔCT method was used (Livak and Schmittgen, 2001).…”

Section: Quantitative Real-time Pcr and Statistical Analysismentioning

confidence: 99%

Nuclear Factor-κB Plays a Positive Role in TNFα Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

Zhu

Guo

et al. 2022

Front. Mar. Sci.

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Tumour necrosis factor-α (TNFα) is a multifarious mediator of lymphoid tissue growth and antimicrobial defence mechanisms, and it acts as a pro-inflammatory regulator. The function of TNFα in parasite infection and the underlying mechanism through which nuclear factor-κB (NF-κB) regulates TNFα remain largely unclear in teleosts. In the present study, TNFα (ToTNFα) from golden pompano (Trachinotus ovatus) was identified, and its sequence features and expression levels were determined. The genomic DNA sequence is composed of 1,130 bp, consists of four exons and three introns, and encodes 341 amino acid polypeptides. The putative protein sequence shares 34.7%–61.9% identity with fish TNFα and possesses a TNF family signature and two conserved cysteine residues. Moreover, the expressions of ToNF-κB and ToTNFα are constitutively expressed in all examined tissues, with higher levels observed in the immune relevant tissues. Both ToNF-κB and ToTNFα transcription was increased in the local infection sites (skin and gill) and system immune tissues (liver, spleen and head kidney) after Cryptocaryon irritans stimulation. In addition, to investigate whether ToNF-κB is a regulator of ToTNFα, promoter analysis was performed. The region from -970 to +79 bp is known as the core promoter by different truncated mutants of ToTNFα. Subsequently, the activity of the ToTNFα-p2 promoter was dramatically reduced after targeted mutation of the M6-binding site. Additionally, an electrophoretic mobile shift assay (EMSA) verified that ToNF-κB interacted with the M6-binding site in the ToTNFα promoter region to control the expression of ToTNFα. In conclusion, the present study provides the positive regulation of TNFα transcription by NF-κB and contributes to a better understanding of the transcriptional mechanism of TNFα in fish.

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The transcriptional factor GATA-4 negatively regulates Hsp70 transcription in Crassostrea hongkongensis

Hou

Cui

et al. 2020

Mol Biol Rep

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The guanine nucleotide-binding protein α subunit protein ChGnaq positively regulates Hsc70 transcription in Crassostrea hongkongensis

Cited by 6 publications

References 41 publications

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758)

Nuclear Factor-κB Plays a Positive Role in TNFα Expression in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

The transcriptional factor GATA-4 negatively regulates Hsp70 transcription in Crassostrea hongkongensis

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