The H3K27me3 Demethylase RELATIVE OF EARLY FLOWERING6 Suppresses Seed Dormancy by Inducing Abscisic Acid Catabolism

Chen, Huhui; Tong, Jianhua; Fu, Wei; Liang, Zeyin; Ruan, Jiuxiao; Yu, Yaoguang; Song, Xin; Yuan, Lei; Xiao, Langtao; Li, Jun; Cui, Yuhai; Huang, Shangzhi; Li, Chenlong

doi:10.1104/pp.20.01255

Cited by 42 publications

(40 citation statements)

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“…In contrast, genes marked by triple repressive modifications remained silenced during germination, because REF6 binding is repelled by H3K9me2/CHGm, consistent with previous work(Qiu et al, 2019). Supporting the functional requirement of REF6 to initiate germination, loss of REF6 caused higher seed dormancy (Figure 3 and Figure 3—figure supplement 2)(Chen et al, 2020), connected with decreased expression of many germination-inducible genes. Loss of SUVH4/5/6 also increased dormancy (Figure 6—figure supplement 1) (Zheng et al, 2012), which was associated with increased expression of potential REF6 target genes.…”

Section: Discussionmentioning

confidence: 69%

“…We confirmed significant suppression of EDF4 in the ref6 mutant endosperm (Figure 5d), supporting the proposed endosperm-specific function of REF6 during germination (Figure 4a and 4b). REF6 was shown to target the ABA catabolism genes CYP707A1 and CYP707A3 , but not CYP707A2 during seed development(Chen et al, 2020). We also found CYP707A1 and CYP707A3 slightly, but significantly suppressed in ref6 endosperm (Figure 5—figure supplement 3), consistent with the presence of REF6-BS in CYP707A1 and CYP707A3 , but not CYP707A2 .…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, also REF6 is not imprinted (Hsieh et al, 2011; Pignatta et al, 2014) but ref6 had a maternal effect (Figure 3b), suggesting that derepression of some dormancy-controlling genes starts in the central cell of the female gametophyte. The REF6 targets CYP707A1 and CYP707A3 (Chen et al, 2020) are potentially activated by REF6 in the central cell, since they lack H3K27me3 in 4 DAP endosperm. Thus, removal of H3K27me3 before fertilization may explain maternally-controlled dormancy.…”

Section: Discussionmentioning

confidence: 99%

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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancy

Sato

Santos‐González

Köhler

2020

Preprint

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Polycomb Repressive Complex 2 (PRC2)-mediated trimethylation of histone H3 on lysine 27 (H3K27me3) and methylation of histone 3 on lysine 9 (H3K9me) are two repressive epigenetic modifications that are typically localized in distinct regions of the genome. For reasons unknown, however, they co-occur in some organisms and special tissue types. In this study, we show that maternal alleles marked by H3K27me3 in the Arabidopsis endosperm were targeted by the H3K27me3 demethylase REF6 and became activated during germination. In contrast, maternal alleles marked by H3K27me3, H3K9me2, and CHGm were protected from REF6 targeting and remained silenced. Our study unveils that combinations of different repressive epigenetic modifications time a key adaptive trait by modulating access of REF6.

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Section: Discussionmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancy

Sato

Santos‐González

Köhler

2020

Preprint

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“…Abscisic acid plays a crucial role in the dormancy process ( Chen et al, 2020b ; Li et al, 2018 ; Song, Zhu & Yan, 2020 ; Tylewicz et al, 2018 ; Zheng et al, 2018 ). Environmental variations cause fluctuations of endogenous ABA levels in plants, resulting in multifarious physiological responses.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, characterisation, and evolution of ABF/AREB subfamily in nine Rosaceae species and expression analysis in mei (Prunus mume)

Xue

Zheng

Zhuo

et al. 2021

PeerJ

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Rosaceae is an important family containing some of the highly evolved fruit and ornamental plants. Abiotic stress responses play key roles in the seasonal growth and development of plants. However, the molecular basis of stress responses remains largely unknown in Rosaceae. Abscisic acid (ABA) is a stress hormone involving abiotic stress response pathways. The ABRE-binding factor/ABA-responsive element-binding protein (ABF/AREB) is a subfamily of the basic domain/leucine zipper (bZIP) transcription factor family. It plays an important role in the ABA-mediated signaling pathway. Here, we analyzed the ABF/AREB subfamily genes in nine Rosaceae species. A total of 64 ABF/AREB genes were identified, including 18, 28, and 18 genes in the Rosoideae, Amygdaloideae, and Maloideae traditional subfamilies, respectively. The evolutionary relationship of the ABF/AREB subfamily genes was studied through the phylogenetic analysis, the gene structure and conserved motif composition, Ka/Ks values, and interspecies colinearity. These gene sets were clustered into four groups. In the Prunus ABF/AREB (PmABF) promoters, several cis-elements related to light, hormone, and abiotic stress response were predicted. PmABFs expressed in five different tissues, except PmABF5, which expressed only in buds. In the dormancy stages, PmABF1, 2, 5 and 7 showed differential expression. The expression of PmABF3, 4 and 6 was positively correlated with the ABA concentration. Except for PmABF5, all the PmABFs were sensitive to ABA. Several ABRE elements were contained in the promoters of PmABF1, 3, 6, 7. Based on the findings of our study, we speculate that PmABFs may play a role in flower bud dormancy in P. mume.

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“…Chromatin modification plays an essential role in transcriptional regulation of many physiological processes. In a recent study, Chen et al reported that histone demethylation by the methyltransferase RELATIVE OF EARLY FLOWERING 6 (REF6) reduces seed dormancy by inducing ABA catabolism in seeds [ 102 ]. REF6 catalyzes the lysine (K27) demethylation of histone H3 (H3K27m3), of which methylation is associated with gene silencing.…”

Section: Aba Metabolism and Seed Dormancy Inductionmentioning

confidence: 99%

ABA Metabolism and Homeostasis in Seed Dormancy and Germination

Sano

Marion‐Poll

2021

IJMS

136

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Abscisic acid (ABA) is a key hormone that promotes dormancy during seed development on the mother plant and after seed dispersal participates in the control of dormancy release and germination in response to environmental signals. The modulation of ABA endogenous levels is largely achieved by fine-tuning, in the different seed tissues, hormone synthesis by cleavage of carotenoid precursors and inactivation by 8′-hydroxylation. In this review, we provide an overview of the current knowledge on ABA metabolism in developing and germinating seeds; notably, how environmental signals such as light, temperature and nitrate control seed dormancy through the adjustment of hormone levels. A number of regulatory factors have been recently identified which functional relationships with major transcription factors, such as ABA INSENSITIVE3 (ABI3), ABI4 and ABI5, have an essential role in the control of seed ABA levels. The increasing importance of epigenetic mechanisms in the regulation of ABA metabolism gene expression is also described. In the last section, we give an overview of natural variations of ABA metabolism genes and their effects on seed germination, which could be useful both in future studies to better understand the regulation of ABA metabolism and to identify candidates as breeding materials for improving germination properties.

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The H3K27me3 Demethylase RELATIVE OF EARLY FLOWERING6 Suppresses Seed Dormancy by Inducing Abscisic Acid Catabolism

Abstract: H3K27me3 demethylase REF6 suppresses seed dormancy and promotes seed germination by enhancing the catabolism of plant hormone Abscisic acid (ABA).

Cited by 42 publications

References 49 publications

Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancy

Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancy

Genome-wide identification, characterisation, and evolution of ABF/AREB subfamily in nine Rosaceae species and expression analysis in mei (Prunus mume)

ABA Metabolism and Homeostasis in Seed Dormancy and Germination

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