The article presents data on the diversity and substrate groups of bryoflora of the Znesinnya Regional Landscape Park (Znesinnya RLP), located in the city of Lviv (Western Ukraine). Based on field research carried out in the period 2015-2018 and analysis of herbarium collections, an inventory was made of the bryoflora of the Znesinnya RLP. A total of 113 species of bryoflora belonging to 66 genera, 35 families and 2 divisions are presented from the investigated area. Of these, 105 species are members of the division Bryophyta and 8 belong to Marchantiophyta. Six regionally rare species of bryophytes have been recorded, namely Pellia endiviifolia, P. epiphylla, Encalypta streptocarpa, Fissidens exilis, Cirriphyllum crassinervium and Sciurohypnum starkei. With regard to substrate preferences, epigeous species of bryophytes predominated and accounted for 89.0% of the total number of species. The largest proportion of bryophytes occurred on bare soil (46.0%), while 36.3% and 25.7% species were found on soil among herbaceous vegetation and on soil with gravel, respectively. Stony substrates were colonized by 42.5% of bryophyte species, with 19.5% of species occurring on artificial stony substrates. In addition, 24.8% of the species belonged to epixils inhabiting old stumps and logs of varying degrees of decay, and the same proportion was represented by epiphytic species of bryophytes. The smallest proportion (10.7%) of bryophytes was confined to water bodies and swampy ecotopes.