The Haematology Analyser SF-3000: performance of the automated white blood cell differential count in comparison to the Haematology Analyser NE-1500

Abstract

The present study evaluates the performance of automated white blood cell (WBC) differential counts by the new Haematology Analyser SF-3000. Five hundred and sixty-six WBC differential counts performed by the SF-3000 were compared with WBC differential counts of the well established analyser NE-1500 and to manual reference counts. Numerical results of the WBC differential counts were correlated to each other by regression analyses. The efficiency of instrument flagging for the presence of abnormal WBC was expressed as per cent of subjects correctly classified. Neutrophil and lymphocyte counts correlated well between analysers and to manual reference counts. Monocyte counts for the SF-3000 correlated significantly better with the microscopic counts, whereas correlations of eosinophils and basophils were better for the NE-1500. The efficiency rates of flagging for the presence of > or = 1% abnormal WBC were 80% for the NE-1500 and 70% for the SF-3000. This difference was exclusively due to low specificity of the SF-3000 in flagging cells of the 'Left Shift' category, especially in samples with elevated WBC counts. The flagging efficiencies for blasts, promyelocytes, myelocytes, atypical lymphocytes and nucleated red cells were identical for both analysers. Thus, with regard to the performance of automated WBC differential counts the SF-3000 seems comparable with other, well established haematology analysers.

New quantitative parameters on a recently introduced automated blood cell counter - the XE 2100TM

“…1999 ), the R series of automated reticulocyte counters ( Tichelli et al . 1990 ) and the SF 3000 ( Korninger et al . 1998 ).…”

“…The XE 2100 (Sysmex Corporation, Japan) is a recently introduced fully automated blood cell counter that utilizes new technology to extend the range of quantitative cell analyses to include a nucleated red cell count, an immature granulocyte count (metamyelocytes, myelocytes and promyelocytes) and an optical¯uorescent platelet count in situations where an impedance count may be unreliable. The analyser is a development from earlier Sysmex systems, particularly the SE 9000 and 9500 (Besson 1996 1 1 ;Pollard et al 1999), the R series of automated reticulocyte counters (Tichelli et al 1990) and the SF 3000 (Korninger et al 1998).…”

Single administration effects of ethanol on the distribution of white blood cells in rats

“…Count analyses of WBCs were carried out by the hematology analyzer (model SF-3000, Sysmex Co.) based on a flow cytometry technique with light-emitting diode (Korninger et al, 1998).…”

Acute and Subacute Effects of Dexamethasone on the Number of White Blood Cells in Rats

“…1A). 5,17,[22][23][24][25] Plasma immunoglobulin G (IgG) concentrations were assayed by our routine method of enzyme-linked immunosorbent assay (ELISA) until 24 hr after dexamethasone administration. Subacute Effects of Dexamethasone on the Number of WBCs --After 5 days of prefeeding, the rats were divided into dexamethasone (dose = 1.0 mg/kg BW per day, n = 11, the initial BW = 259 ± 3 g, mean ± SEM) and control groups (n = 10, the initial BW = 260 ± 2 g, mean ± SEM).…”