The Haemodynamic Effects of Metabolic Acidosis in the Rat

Yudkin, John; Cohen, Robert; Slack, Barbara

doi:10.1042/cs0500177

Cited by 18 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, as shown by Yudkin et al [21], the increase in HCO3 observed during metabolic acidosis in all the studied groups could be due to intrarenal hemodynamic changes, which could led to modifications in the urinary flux.…”

Section: Discussionmentioning

confidence: 61%

Functional and morphometric evaluation of offspring kidney after intrauterine undernutrition

et al. 1997

View full text Add to dashboard Cite

Pregnant rats were subjected to 50% food restriction during the first or the second half of pregnancy, or throughout pregnancy. The effects of intrauterine food restriction, on kidney function and morphometry were studied in newborn and adult (3 months) offspring. No differences in glomerular diameter were observed in newborn restricted rats compared with controls. The number of glomeruli was significantly lower both in newborn and 3-month-old restricted rats. However, glomerular diameter was increased in 3-month-old rats, which suggests that hypertrophic stimuli were present. The medulla/cortex ratio increased in adult rats submitted to food restriction during pregnancy, a finding that agrees with the preserved sodium and acid excretion, and the normal osmolar and free water clearance observed in these groups. These results show that the reduction in glomerular number is still present 3 months after birth in the progeny of mothers submitted to severe food restriction during pregnancy, suggesting impairment of glomerulogenesis even after birth. Intra utero undernutrition can be regarded as an experimental model of glomerular hypertrophy.

show abstract

Section: Discussionmentioning

confidence: 61%

Functional and morphometric evaluation of offspring kidney after intrauterine undernutrition

et al. 1997

View full text Add to dashboard Cite

show abstract

“…The liver was perfused during 30 rnin at a flow rate of 12 ml min-' (1OOg body weight)-' which is considered as the 'normal' (100%) flow rate [27]. The 'low' flow rate corresponds to 25% of the normal value (hypoxic conditions).…”

Section: Effect Of Ethanol On the Energetic Metabolism Of'the Liver Omentioning

confidence: 99%

Phosphorus‐31 nuclear‐magnetic‐resonance study of phosphorylated metabolites compartmentation, intracellular pH and phosphorylation state during normoxia, hypoxia and ethanol perfusion, in the perfused rat liver

Desmoulin

Cozzone

Canioni

1987

European Journal of Biochemistry

View full text Add to dashboard Cite

A quantitative analysis of the phosphorus-31 NMR spectra of excised perfused rat liver has been carried out at 80.9 MHz using a 30-mm sample cell.1. The results indicate that in liver from fed rats, all intracellular ATP is detected by NMR. In contrast, only the cytosolic fractions of Pi and ADP can be observed as indicated by careful analysis of spectra obtained from perchloric acid liver extracts and intact liver under valinomycin perfusion. In well-oxygenated perfused liver the ATP concentration is 7.4 mM. Values of 5.3 mM and 0.9 mM are found respectively for Pi and ADP concentrations in the cytosolic compartment. Cytosolic pH value (pH,) is 7.25 k 0.05 and free magnesium concentration 0.5 mM.2. Addition of 70 mM (0.4%) ethanol to the perfusate of a fed rat liver induces 25% and 38% reduction of ATP and Pi levels, respectively. A large amount of sn-glycerol 3-phosphate is synthesized (up to 11 mM) in the cytosol. After ethanol withdrawal, a large overshoot in cytosolic Pi is observed, which is indicative of a net uptake of Pi across the plasma membrane that occurred during ethanol oxidation. No significant pH variation is observed during ethanol infusion.3. In perfused liver of rats subjected to 48-h fasts, the concentrations of cytosolic phosphorylated metabolites are 5.3 mM, 0.8 mM and 11.5 mM for ATP, ADP and Pi, respectively. The perfusion of the liver with 70 mM ethanol does not change the adenine nucleotide levels, while the Pi content is decreased by 10%. 4.During a 4-min hypoxia, induced by reducing the perfusion flow rate from 12 ml to 3 ml min-(100 g body weight)-', ATP concentration decreases to 5.8 mM in the fed rat liver. Cytosolic Pi and ADP increase to 8.7 mM and 1.6 mM, respectively. The cytosolic pH evolves to more acidic values and reaches 7.02 k 0.05 at the end of the 4-min hypoxic period.Studies on the hepatic metabolism by phosphorus-31 nuclear magnetic resonance spectroscopy (31P NMR) were initiated on isolated rat liver cells in 1978 by Cohen et al. [I]. The technique has been subsequently applied to the perfused rat liver [2] and the mouse liver [3]. Since high-energy phosphates (ATP) and inorganic phosphate (Pi) are the main metabolites giving rise to well-resolved signals in perfused liver spectra, reports have been generally limited to experimental situations where drastic changes in the energetic metabolism of the organ are induced, e.g. cyanide intoxication [2], ischemia [3, 41 and fructose infusion [5, 61. An extensive study of perfused liver by simultaneous observation of 13C and 31P nuclei has been published by Cohen [7]. More reCorrespondence to P. J. Cozzone, UA CNRS 11 86,

show abstract

“…70000 to an Hct of 18-20%. Poor correction of lactic acidosis when treating hemorrhagic shock with crystalloids as opposed to colloids may lead to adverse hemodynamic effects, such as decreased cardiac contractility [9,36], and to a more modest increase of cardiac output.…”

Section: Discussionmentioning

confidence: 99%

Correction of hemorrhagic shock-induced liver hypoxia with whole blood, Ringer's solution or with hetastarch

et al. 1989

View full text Add to dashboard Cite

Liver oxygenation was studied with hemorrhagic hypotension and corrected using whole blood, a synthetic colloid (hydroxyethyl starch or hetastarch, HES; mol. wt. 120,000), or a crystalloid solution. Measurements were performed directly by recording pig liver tissue oxygen tension with an implanted silicone elastomer (Silastic) tube, and indirectly by calculating blood oxygen contributions. The direct method seems fairly reliable and accurately reflects different levels of bleeding and shock and their correction. Liver tissue oxygen tension (PlO2) may thus be used as an indicator of central organ response to shock management. PlO2 decreased during bleeding from 33.5 +/- 0.5 to 16.0 +/- 0.5 torr, and normalized rapidly after retransfusion. The baseline values were significantly exceeded after hetastarch infusion but were never reached with Ringer's solution. The correction of liver oxygen consumption was less complete after crystalloid infusion as well. On the other hand, the difference in liver oxygenation was less marked after crystalloid infusion and retransfusion, which restored perfusion to the baseline. The total amount of Ringer's solution needed to keep the animals hemodynamically stable during the 2-h follow-up period was four times higher than with hetastarch and some five times the blood volume shed. The cause of defective correction of liver oxygenation seems to be the poor response of liver blood flow to refilling in the Ringer group, in addition to apparent tissue edema after crystalloid infusion. According to our study, hemorrhagic hypotension related to liver oxygenation is more promptly and completely corrected with the colloid hydroxyethyl starch than with a crystalloid solution in the early phase of treatment.

show abstract

The Haemodynamic Effects of Metabolic Acidosis in the Rat

Cited by 18 publications

References 8 publications

Functional and morphometric evaluation of offspring kidney after intrauterine undernutrition

Functional and morphometric evaluation of offspring kidney after intrauterine undernutrition

Phosphorus‐31 nuclear‐magnetic‐resonance study of phosphorylated metabolites compartmentation, intracellular pH and phosphorylation state during normoxia, hypoxia and ethanol perfusion, in the perfused rat liver

Correction of hemorrhagic shock-induced liver hypoxia with whole blood, Ringer's solution or with hetastarch

Contact Info

Product

Resources

About