The Halobacteriaceae

Kushner, D. J.

doi:10.1016/b978-0-12-307208-5.50009-x

Cited by 89 publications

(93 citation statements)

References 171 publications

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“…Salts at high concentration play a major role in the structure and stability of halophilic proteins [1][2][3]. We have shown before that nucleoside diphosphate kinase from Halobacterium salinarum (HsNDK), once folded, maintains the native and active structure at 0.2 M NaCl [4].…”

Section: Introductionmentioning

confidence: 99%

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A single Gly114Arg mutation stabilizes the hexameric subunit assembly and changes the substrate specificity of halo‐archaeal nucleoside diphosphate kinase

Ishibashi¹,

Tatsuda²,

Izutsu³

et al. 2007

FEBS Letters

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Nucleoside diphosphate kinase from extremely halophilic archaeon (HsNDK) requires above 2 M NaCl concentration for in vitro refolding. Here an attempt was made to isolate mutations that allow HsNDK to refold in low salt media. Such a screening resulted in isolation of an HsNDK mutant, Gly114Arg, which efficiently refolded in the presence of 1 M NaCl. This mutant, unlike the wild type enzyme, was expressed in Escherichia coli as an active form. The residue 114 is in close proximity to Glu155 of the neighboring subunit in the three dimensional hexameric structure of the HsNDK. It is thus possible that the attractive electrostatic interactions occur between Arg114 and Glu155 in the mutant HsNDK, stabilizing the hexameric subunit assembly.

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Section: Introductionmentioning

confidence: 99%

“…above 2 M [5]. Halophilic proteins are characterized by abundant acidic amino acids, especially on the protein surface [1][2][3][6][7][8]. High net charges at the protein surface, when not neutralized, generate unfavorable electrostatic free energy and destabilize the proteins [9].…”

Section: Introductionmentioning

confidence: 99%

A single Gly114Arg mutation stabilizes the hexameric subunit assembly and changes the substrate specificity of halo‐archaeal nucleoside diphosphate kinase

Ishibashi¹,

Tatsuda²,

Izutsu³

et al. 2007

FEBS Letters

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“…Vibrio costicola is a bacterium which is able to grow over a wide range of salt concentrations and thus is included in the moderately halophilic category (11). This species was isolated and described for the first time by Smith (24), as " V .…”

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Taxonomic Study and Amended Description of Vibrio costicola

Garcı́a

Ventosa

Ruiz‐Berraquero

et al. 1987

International Journal of Systematic Bacteriology

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A total of 54 moderately halophilic vibrios, which were isolated from several salterns located in different areas of Spain, were examined by using a wide range of morphological, physiological, biochemical, and nutritional tests. The resulting data, together with data for four reference Vibrio costicola strains including the type strain V. costicolu NCMB 701, and other marine species that were similarly examined, were compared by using several numerical taxonomic methods. There was a strong similarity between the 54 isolates and four reference strains of V. costicola that were isolated from cured meats. On the basis of these and other molecular data, including guanine-plus-cytosine content of the deoxyribonucleic acid and the plasmid content, we propose an amended description of this species.Vibrio costicola is a bacterium which is able to grow over a wide range of salt concentrations and thus is included in the moderately halophilic category (11). This species was isolated and described for the first time by Smith (24), as " V . costicolus," from rib bones in certain Australian bacon, suggesting the name "costicolus" (rib dweller). At present, V. costicola is included as a valid species in the Approved Lists of Bacterial Names (23).From a physiological point of view, many studies have been carried out with this species, and in fact, it has been considered as an organism representative of moderately halophilic bacteria. However, only a few taxonomic studies have been carried out, and all of them have studied organisms isolated from cured meats and curing brines (7,24,29; J. Robinson, Ph.D. thesis, McGill University, 1950).In the Bergey 's Manual of Determinative Bacteriology (8th ed.), this species was included as a member of the genus Vibrio and named correctly as V. costicola, but the taxonomic description was not very exhaustive (22). The recent edition of Bergey 's Manual of Systematic Bacteriology also includes this species in the genus Vibrio and provides more phenotypic characteristics, especially nutritional tests, but is based only upon two strains isolated from cured meats (1).In a taxonomic study of gram-negative rods isolated from solar salterns, Ventosa et al. (28) showed that microorganisms with phenotypic characteristics similar to those of V . costicola were present in high proportions in some ponds of the salterns. In the present work we isolated and characterized 54 vibrios from several hypersaline habitats and studied them in depth, together with the type strain V . costicola NCMB 701T as well as some other strains of this species isolated from cured meats and other reference strains. MATERIALS AND METHODSBacterial strains. The 54 strains studied were isolated from several solar salterns in Spain, located near Alicante, Huelva, and Cadiz, in the southeast, south, and southwest of Spain, respectively, and from the Canary Islands (Spain) in the Atlantic Ocean. The isolation medium, as well as the methodology, has been previously described (28). The selection of the strains was made on the basis ...

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“…The Acinetobacter 162 -PART 2. DOCUMENTS ON MICRO-ORGANISMS SAFETY ASSESSMENT OF TRANSGENIC ORGANISMS: OECD CONSENSUS DOCUMENTS: VOLUME 4 © OECD 2010 genus is generally considered as moderately halophilic as species grow optimally in media containing 0.5-2.5M salt (Kushner, 1985). A number of Acinetobacter strains have been isolated from marine environments (Puchenkova, 1988;Bruni et al, 1999;Xie et al, 2005), a salt marsh (Buchan et al, 2001), and an inland saltern (del Moral et al, 1987).…”

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Safety Assessment of Transgenic Organisms, Volume 4

2010

Harmonisation of Regulatory Oversight in Biotechnology

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io s a fe t y nv ir on me nt ag ri cu lt ur e bi os af et y g r ic u lt u r e e n vi r o n m e n t gr ic ul tu re bi os af et y en vi ro nm en t n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en v io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f gr ic ul tu re bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t a n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u io sa fe ty en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e nv ir on me nt ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y e g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a fe t y a g r ic u lt u r e e n vi r o n m e n t b io s a f ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et y en vi ro nm en t ag ri cu lt ur e bi os af et...

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The Halobacteriaceae

Cited by 89 publications

References 171 publications

A single Gly114Arg mutation stabilizes the hexameric subunit assembly and changes the substrate specificity of halo‐archaeal nucleoside diphosphate kinase

A single Gly114Arg mutation stabilizes the hexameric subunit assembly and changes the substrate specificity of halo‐archaeal nucleoside diphosphate kinase

Taxonomic Study and Amended Description of Vibrio costicola

Safety Assessment of Transgenic Organisms, Volume 4

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