Abstract-The aim of this study was to evaluate the influence of thermocycling on the mechanical properties of a commercial dental nanocomposite by using the nano-indentation experiment. The resin-based nanocomposite contained silica and zirconia nanofillers. Some disk specimens, each with the diameter of 10 mm and the thickness of 4 mm were prepared and the specimens were stored at various environmental conditions. Some of the samples were kept in the ambient condition, other samples were stored in distilled water at room temperature and other ones were thermocycled in distilled water for 1000 cycles between 5°C and 55°C. After preparation of samples, the nano-indentation test was applied on the prepared specimens by Triboscope setup. Then the modulus of elasticity and hardness of the test samples were calculated from the Oliver-Pharr's method using the data obtained from nano-indentation test. The present research showed that the samples stored in distilled water without thermocycling exhibited the highest elasticity modulus when compared to the other samples. In addition, the maximum hardness was observed for thermocycled specimens in distilled water.