The HC-Pro Protein of Potato Virus Y Interacts with NtMinD of Tobacco

Abstract

Potato virus Y (PVY) infections often lead to altered numbers of host plant chloroplasts, as well as changes in morphology and inhibited photosynthesis. The multifunctional protein helper component-proteinase, HC-Pro, has been identified in PVY-infected leaf chloroplasts. We used yeast two-hybrid and bimolecular fluorescence complementation assays to demonstrate that HC-Pro can interact with the chloroplast division-related factor NtMinD in yeast and tobacco cells, respectively. In addition, we confirmed that residues 271 to 314 in NtMinD are necessary for its interaction with PVY HC-Pro in a yeast two-hybrid analysis using four NtMinD deletion mutants. These residues are necessary for the dimerization of NtMinD, which plays a vital role in chloroplast division. Thus, PVY HC-Pro may affect NtMinD activity by inhibiting the formation of NtMinD homodimers, and this may interfere with chloroplast division and contribute to changes in the numbers of chloroplast per cell observed in PVY-infected plants.

Selective Interaction Between Chloroplast β-ATPase and TGB1L88 Retards Severe Symptoms Caused by Alternanthera mosaic virus Infection

“…Examples include interactions of the Rubisco large subunit with Potato virus Y (PVY) CP (Feki et al, 2005), and chloroplast division-related protein MinD with PVY HC-Pro (Jin et al, 2007). Other potyviral interactions include sugarcane ferredoxin-5 with Sugarcane mosaic virus HC-Pro (Cheng et al, 2008), and Rieske Fe/S protein of several hosts with the P1 protein of Soybean mosaic virus (Shi et al, 2007).…”

Helper Component Proteinase of the Genus Potyvirus Is an Interaction Partner of Translation Initiation Factors eIF(iso)4E and eIF4E and Contains a 4E Binding Motif

“…HCpro of Lettuce mosaic virus (LMV) interferes and interacts with the 20S proteasome complex in cauliflower (Brassica oleracea) (6), whereas HCpro of Potato virus Y (PVY) interacts with several proteasome-subunits of Arabidopsis thaliana (35). The chloroplast division-related factor NtMinD of tobacco binds to HCpro of PVY (36), and a chloroplast precursor of ferredoxin-5 binds to HCpro of Sugar cane mosaic virus (SCMV) in maize (Zea mays) (15). A calmodulin-like cellular regulator of RNA silencing and the ethylene-inducible transcription factor RAV2 in tobacco (N. tabacum) interact with HCpro (2,23).…”

Sensing of biomolecular interactions using fluorescence complementing systems in living cells

“…BiFC technology has also been utilized in study of plant viruses, such as in vitro and in vivo mapping of the Prunus necrotic ring spot virus coat protein C-terminal dimerization domain (Aparicio et al, 2006), visualization of the interaction between the precursors of the viral protein linked to the genome of Turnip mosaic virus and the translation eukaryotic initiation factor iso 4E in plants (Beauchemin et al, 2007), interaction of the helper component-proteinase of Potato virus Y interacts with the chloroplast division-related factor of tobacco (Jin et al, 2007), live-cell imaging of viral RNA genomes of tobacco mosaic virus (TMV) (Tilsner et al, 2009), an integrated protein localization and interaction map for Potato yellow dwarf virus (Bandyopadhyay et al, 2010), nuclear localization of Beet black scorch virus capsid protein and its interaction with importin alpha , interaction of the trans-frame Potyvirus protein with host protein PCaP1 and its role in facilitating Potyvirus movement (Vijayapalani et al, 2012), in vivo membrane association of the movement protein (NSm) of the tospovirus species Bean necrotic mosaic virus, Chrysanthemum stem necrosis virus, Tomato chlorotic spot virus and Tomato spotted wilt virus and the homologous and heterologous interactions among NSm and nucleocapsid protein (Leastro et al, 2015).…”