The Healing Effect of Amniotic Membrane in Laryngeal Defects in Rabbit Model

Abstract

Objective Fibrosis and scar formation following laryngeal repairing is a major concern. This study evaluated the efficacy of applying amniotic membrane (AM) as a biologic dressing to reduce fibrosis and inflammation and promote cartilage repair. Study design Experimental animal study. Methods Nine male white Dutch rabbits were selected. A standard defect (5 mm) was created in right and left thyroid lamina. In the right side, two layers of AM were applied while the left side was kept intact for future comparison. Histologic examination with criteria of inflammation, fibrosis, and cartilage regeneration was performed 2, 4, and 6 weeks following AM application. All procedures were done according to animal ethics rules. Results Histologic and gross examination showed that AM application was able to reduce inflammation and fibrosis and improve cartilage regeneration significantly. Conclusion As a useful intervention, AM application can reduce inflammation and fibrosis and enhance cartilage regeneration, following laryngeal defect repair. Level of Evidence NA Laryngoscope, 131:E527–E533, 2021

Use of Magnetic Resonance Imaging to Assess the Regenerative Effects of Adipose Tissue-Derived Mesenchymal Stem Cells in a Rabbit Cartilaginous Laryngeal Defect Model

“…This study utilized some previously described non-MRI methods to evaluate animal cartilage regeneration. 22 …”

“…Fibrosis was assessed by the thickness of fibrous tissue on the micrometer scale in the defect area. 22 , 37 Histologic examination was done by an expert pathologist masked as to the treatment side. Histologic criteria grading is shown in Table 1 .…”

Applications of the amniotic membrane in tissue engineering and regeneration: the hundred-year challenge

“…The AMSCs possess unique characteristics that render them useful in diverse applications for tissue repair. These include using as cartilage grafts for tracheal reconstruction of the fetus [ 51 , 52 ], restoration for the diaphragm muscles [ 53 , 54 ], bone grafts [ 55 , 56 ], and heart valve leaflets [ 57 – 59 ]. Furthermore, seeding human AMSCs in gelatin microcarriers could successfully generate modular bone-like tissues upon osteogenic differentiation [ 60 ].…”

Effect of amniotic membrane/collagen scaffolds on laryngeal cartilage repair

“…Various treatment modalities, materials, and drugs have been introduced to facilitate laryngeal cartilaginous healing and prevent complications. Several clinical and experimental studies have shown different degrees of success and limitations of these treatment modalities, such as the use of amniotic membrane (AM), 2 adipose‐derived stem cells (ASCs), 3 seeded scaffolds, 4 and platelet‐rich plasma (PRP) 5 in laryngeal defects repair.…”

“…as the use of amniotic membrane (AM), 2 adipose-derived stem cells (ASCs), 3 seeded scaffolds, 4 and platelet-rich plasma (PRP) 5 in laryngeal defects repair.…”