The healing of critical-size calvarial bone defects in rat with rhPDGF-BB, BMSCs, and β-TCP scaffolds

Xu, Ling; Lv, Kaige; Zhang, Wenjie; Zhang, Xiuli; Jiang, Xinquan; Zhang, Fuqiang

doi:10.1007/s10856-012-4558-x

Cited by 56 publications

(49 citation statements)

References 40 publications

(40 reference statements)

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“…1,2 Although alveolar bones have the capability to regenerate, the healing process frequently fails to progress through the normal stages of healing or is delayed when the defects reach a critical size. [3][4][5] Therefore, bone grafting is necessary for large defects.…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Du¹,

Liu²,

Deng³

et al. 2015

IJN

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To improve the regenerative performance of nano-hydroxyapatite/coralline (nHA/coral) block grafting in a canine mandibular critical-size defect model, nHA/coral blocks were coated with recombinant human vascular endothelial growth factor 165 (rhVEGF) via physical adsorption (3 μg rhVEGF 165 per nHA/coral block). After the nHA/coral blocks and VEGF/nHA/coral blocks were randomly implanted into the mandibular box-shaped defects in a split-mouth design, the healing process was evaluated by histological observation and histomorphometric and immunohistological analyses. The histological evaluations revealed the ingrowth of newly formed blood vessels and bone at the periphery and cores of the blocks in both groups at both 3 and 8 weeks postsurgery, respectively. In the histomorphometric analysis, the VEGF/nHA/coral group exhibited a larger quantity of new bone formation at 3 and 8 weeks postsurgery. The percentages of newly formed bone within the entire blocks in the VEGF/nHA/coral group were 27.3%±8.1% and 39.3%±12.8% at 3 weeks and 8 weeks, respectively, and these values were slightly greater than those of the nHA/coral group (21.7%±3.0% and 32.6%±10.3%, respectively), but the differences were not significant ( P >0.05). The immunohistological evaluations revealed that the neovascular density in the VEGF/nHA/coral group (146±32.9 vessel/mm 2 ) was much greater than that in the nHA/coral group (105±51.8 vessel/mm 2 ) at the 3-week time point ( P <0.05), but no significant difference was observed at the 8-week time point (341±86.1 and 269±50.7 vessel/mm 2 , respectively, P >0.05). The present study indicated that nHA/coral blocks might be optimal scaffolds for block grafting in critical-size mandibular defects and that additional VEGF coating via physical adsorption can promote angiogenesis in the early stage of bone healing, which suggests that prevascularized nHA/coral blocks have significant potential as a bioactive material for bone regeneration in large-scale alveolar defects.

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Section: Introductionmentioning

confidence: 99%

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Du¹,

Liu²,

Deng³

et al. 2015

IJN

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“…To determine the release rate of rhPDGF-BB from LPMS and LPMSCs, the scaffolds were soaked in PBS at 37°C. 25 Then, 100 μL of supernatant was repair of calvarial defect in rats…”

Section: Release Of Recombinant Human Pdgf-bb (Rhpdgf-bb) From Scaffoldsmentioning

confidence: 99%

“…As described in our previous study, 25 two circular defects (5 mm in diameter) were created in both sides of rat calvarial bone after anesthesia. The defects were randomly filled with the following implants: LPMS, LPMSC-1, LPMSC-2, and LPMSC-3 alone.…”

Section: Release Of Recombinant Human Pdgf-bb (Rhpdgf-bb) From Scaffoldsmentioning

confidence: 99%

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Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration

Zeng

Zhang

Wang

et al. 2017

IJN

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Our previous study revealed that mesoporous Ca-Si-based materials exhibited excellent osteoconduction because dissolved ions could form a layer of hydroxycarbonate apatite on the surface of the materials. However, the biological mechanisms underlying bone regeneration were largely unknown. The main aim of this study was to evaluate the osteogenic ability of large-pore mesoporous Ca-Si-based bioceramics (LPMSCs) by alkaline phosphatase assay, real-time PCR analysis, von Kossa, and alizarin red assay. Compared with large-pore mesoporous silica (LPMS), LPMSCs had a better effect on the osteogenic differentiation of dental pulp cells. LPMSC-2 and LPMSC-3 with higher calcium possessed better osteogenic abilities than LPMSC-1, which may be related to the calcium-sensing receptor pathway. Furthermore, the loading capacity for recombinant human platelet-derived growth factor-BB was satisfactory in LPMSCs. In vivo, the areas of new bone formation in the calvarial defect repair were increased in the LPMSC-2 and LPMSC-3 groups compared with the LPMSC-1 and LPMS groups. We concluded that LPMSC-2 and LPMSC-3 possessed both excellent osteogenic abilities and satisfactory loading capacities, which may be attributed to their moderate Ca/Si molar ratio. Therefore, LPMSCs with moderate Ca/Si molar ratio might be potential alterative grafts for craniomaxillofacial bone regeneration.

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“…Several methods have been used to demonstrate the osteogenic potential of MSC in vivo including transplantation into a calvarial bone defect [23][24][25][26][27] or subcutaneous spaces 17,[28][29][30] . The method we used was useful for examining osteogenic potential because the cells were transplanted into an environment containing bone tissue in vivo without a complicated procedure such as generating a bone defect or fracture.…”

Section: Previous Reports Have Shown That Dfc Isolated From Humanmentioning

confidence: 99%

Osteogenic Potential of Human Dental Follicle Cells on Rat Calvaria

Iwai

Kuyama

Kuboyama

et al. 2013

J. Hard Tissue Biology.

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The objective of this study was to investigate the osteogenic potential of human dental follicle cells (hDFC) in vivo. hDFC were isolated from dental follicle tissue by enzymatic digestion and cultured in growth medium (GM). hDFC were grown in a three-dimensional (3D) culture using gelatin sponges in osteogenic induction medium (OIM) or GM. The cells were transplanted onto calvaria bone of immunodeficient rats (n = 3). Hematoxylin and eosin (H-E) staining and immunohistochemistry were performed, and bone formation was analyzed with micro-computed tomography (micro-CT). H-E staining showed newly formed bone after transplantation of hDFC grown in a 3D culture in OIM. Immunohistochemistry showed BMP-2, Runx2, and Osterix expression in the hDFC transplantation site during the early stage of bone formation. Moreover, micro-CT showed that the transplanted hDFC that were 3D-cultured in OIM promoted good bone quality and bone volume compared to the other two groups. Thus, human dental follicles may be a potentially useful cell source for regenerative therapy.

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The healing of critical-size calvarial bone defects in rat with rhPDGF-BB, BMSCs, and β-TCP scaffolds

Cited by 56 publications

References 40 publications

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Fabrication of large-pore mesoporous Ca-Si-based bioceramics for bone regeneration

Osteogenic Potential of Human Dental Follicle Cells on Rat Calvaria

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