The heat shock protein 40 LeDnaJ regulates stress resistance and indole-3-acetic acid biosynthesis in Lentinula edodes

Abstract

DnaJ proteins, termed heat shock proteins based on their molecular weight, function as molecular chaperones that play critical roles in regulating organism growth and development as well as adaptation to the environment. However, little has been reported on their gene function in higher basidiomycetes. Here, the heat shock protein 40 (LeDnaJ) gene was cloned and characterized from Lentinula edodes. RNA interference was used to explore the function of LeDnaJ in response to heat stress and Trichoderma atroviride. Integration of the target gene into the L. edodes genome was confirmed by Southern blot analysis, and the silence efficiency of LeDnaJ was analyzed by qRT-PCR. The results revealed that LeDnaJ silence caused defects in mycelial growth and resistance to heat stress and T. atroviride, but increased the mycelial density compared with the wild type (WT) strain S606. Additionally, the IAA content showed a more than 10-fold increase in the WT after heat stress, but an about two-fold increase in the two LeDnaJ RNAi transfortants (LeDnaJ-i-6 and LeDnaJ-i-8). Previous study has shown that enhanced IAA (indole-3-acetic acid) content enhanced the thermotolerance of the heat-sensitive strain YS3357. In this study, it was documented that IAA amendments could partly restore the resistance to T. atroviride and thermotolerance of the two LeDnaJ RNAi transformants. Overall, LeDnaJ is nvolved in fungal growth, T. atroviride resistance, and thermotolerance by regulating the IAA biosynthesis in L. edodes.

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

“…The result is consistent with the expression of Hsp70 genes in other mushrooms (Chen et al 2017;Wang et al 2018a;Zhang et al 2016;Zou et al 2018). The silencing of Hsp40 gene, LeDnaJ, in L. edodes defected its resistance to heat stress (Wang et al 2018b), while the over-expression of LeDnaJ gene in L. edodes S606 conferred the strain better tolerance to heat stress (Wang et al 2018a). Overexpression of an Hsp100 gene, PsHsp100, from Pleurotus sajor-caju complemented a thermotolerance defect in hsp104 mutant S. cerevisiae (Lee et al 2006).…”

Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in Lentinula Edodes Thermotolerance

“…8c). And, in our recent study, it was verified that LeDnaJ participate in the thermotolerance of L. edodes via regulating IAA biosynthesis [89].…”

Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

“…According to our previous studies, it was documented that the RNAi of the LeDnaJ07 decreased mycelial growth and the resistance to heat stress, while LeDnaJ07 overexpression enhanced the thermotolerance of L. edodes heatresistant strain S606 (Wang G.Z. et al, 2016, Wang et al, 2018b. However, it is worthy of study whether LeDnaJ07 overexpression regulates the resistance of other L. edodes strains to stresses or not, how LeDnaJ07 regulates the response of L. edodes to thermotolerance.…”

“…To test the effect of LeDnaJ07 overexpression on the resistance of L. edodes against heat stress, thermotolerance assays, involving short-and long-term heat stresses, were performed according to our previous method (Wang et al, 2018b). In the short-term heat stress group, the stress temperature and recovery time were 38 • C and 25 d, respectively.…”

“…To analyze IAA differences between transformant and WT strains, mycelial samples were grinded into fine powder in liquid nitrogen. IAA extraction and measurement methods were performed according to our previous study (Wang et al, 2018b).…”