The helix 0 of endophilin modifies membrane material properties and induces local curvature

Aryal, Chinta M.; Bui, Nhat Nguyen; Khadka, Nawal K.; Song, Likai; Pan, Jianjun

doi:10.1016/j.bbamem.2020.183397

Cited by 10 publications

(3 citation statements)

References 53 publications

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“…Note that the apparent depth of these holes can be influenced by parameters such as scan size and tip geometry. In previous high-resolution scans, we reported bilayer thicknesses of ~3 nm [56][57][58]. However, based on the apparent hole depth of 2 nm observed in this study, which surpasses half the bilayer's thickness, we can reasonably deduce that the holes induced by HttN traverse the entire bilayer.…”

Section: Popc Bilayerscontrasting

confidence: 41%

“…In our research, we harnessed the capabilities of liquid-based AFM to examine an assortment of macromolecules and their impact on the topographical and mechanical characteristics of lipid bilayers. Through our studies, we have unveiled a spectrum of membrane alterations, ranging from the formation of nanometer-sized pores [55], subtle interfacial indentations [56], the emergence of micrometer-sized perforations [57,58], and lipid clustering [59].…”

Section: Introductionmentioning

confidence: 99%

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Elucidating the Influence of Lipid Composition on Bilayer Perturbations Induced by the N-Terminal Region of the Huntingtin Protein

Gamage,

Pan

2023

Biophysica

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Understanding the membrane interactions of the N-terminal 17 residues of the huntingtin protein (HttN) is essential for unraveling its role in cellular processes and its impact on huntingtin misfolding. In this study, we used atomic force microscopy (AFM) to examine the effects of lipid specificity in mediating bilayer perturbations induced by HttN. Across various lipid environments, the peptide consistently induced bilayer disruptions in the form of holes. Notably, our results unveiled that cholesterol enhanced bilayer perturbation induced by HttN, while phosphatidylethanolamine (PE) lipids suppressed hole formation. Furthermore, anionic phosphatidylglycerol (PG) and cardiolipin lipids, along with cholesterol at high concentrations, promoted the formation of double-bilayer patches. This unique structure suggests that the synergy among HttN, anionic lipids, and cholesterol can enhance bilayer fusion, potentially by facilitating lipid intermixing between adjacent bilayers. Additionally, our AFM-based force spectroscopy revealed that HttN enhanced the mechanical stability of lipid bilayers, as evidenced by an elevated bilayer puncture force. These findings illuminate the complex interplay between HttN and lipid membranes and provide useful insights into the role of lipid composition in modulating membrane interactions with the huntingtin protein.

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Section: Popc Bilayerscontrasting

confidence: 41%

Section: Introductionmentioning

confidence: 99%

Elucidating the Influence of Lipid Composition on Bilayer Perturbations Induced by the N-Terminal Region of the Huntingtin Protein

Gamage,

Pan

2023

Biophysica

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“…N-BAR has three major functional regions, including N-terminal amphiphilic helix (H0), amphiphilic helix inserted into helix 1 (H1), and crescent-shaped body formed by the dimerized BAR structural domain. H0 mediates membrane binding, H1 inserts into the membrane and cooperates with the dimerized BAR body to drive membrane curvature ( 26 – 29 ).…”

Section: Subtypes and Structurementioning

confidence: 99%

Biology of endophilin and it’s role in disease

Yang,

Huang,

2023

Front. Immunol.

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Endophilin is an evolutionarily conserved family of protein that involves in a range of intracellular membrane dynamics. This family consists of five isoforms, which are distributed in various tissues. Recent studies have shown that Endophilin regulates diseases pathogenesis, including neurodegenerative diseases, tumors, cardiovascular diseases, and autoimmune diseases. In vivo, it regulates different biological functions such as vesicle endocytosis, mitochondrial morphological changes, apoptosis and autophagosome formation. Functional studies confirmed the role of Endophilin in development and progression of these diseases. In this study, we have comprehensively discussed the complex function of Endophilin and how the family contributes to diseases development. It is hoped that this study will provide new ideas for targeting Endophilin in diseases.

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Probing the interactions of the HIV-1 matrix protein-derived polybasic region with lipid bilayers: insights from AFM imaging and force spectroscopy

Aryal,

Pan

2024

Eur Biophys J

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The helix 0 of endophilin modifies membrane material properties and induces local curvature

Cited by 10 publications

References 53 publications

Elucidating the Influence of Lipid Composition on Bilayer Perturbations Induced by the N-Terminal Region of the Huntingtin Protein

Elucidating the Influence of Lipid Composition on Bilayer Perturbations Induced by the N-Terminal Region of the Huntingtin Protein

Biology of endophilin and it’s role in disease

Probing the interactions of the HIV-1 matrix protein-derived polybasic region with lipid bilayers: insights from AFM imaging and force spectroscopy

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