The HemoCue®, a point of care B-hemoglobin photometer, measures hemoglobin concentrations accurately when mixedin vitro with canine plasma and three hemoglobin-based oxygen carriers (HBOC)

Jahr, Jonathan S.; Lurie, Fedor; Driessen, Bernd; Davis, Jessica A; Gosselin, Robert; Gunther, Robert A.

doi:10.1007/bf03020522

Cited by 50 publications

(27 citation statements)

References 13 publications

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“…Total Hb was measured directly from a drop of arterial blood and plasma Hb was measured using the plasma collected after Hct measurements. Hemocue has been validated for plasma Hb concentrations between 0.2 and 5 g/dL with unencapsulated HBOCs (28). …”

Section: Methodsmentioning

confidence: 99%

Resuscitation from hemorrhagic shock using polymerized hemoglobin compared to blood

Ortiz

Barros

et al. 2014

The American Journal of Emergency Medicine

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The development of an alternative to blood transfusion to treat severe hemorrhage remains a challenge, especially in far forward scenarios when blood is not available. Hemoglobin (Hb) based oxygen (O2) carriers (HBOCs) were developed to address this need. Hemopure® (HBOC-201, bovine Hb glutamer-250, OPK Biotech, Cambridge, MA, USA), one such HBOC, has been approved for clinical use in South Africa and Russia. At the time of its approval, however, few studies aimed to understand Hemopure’s function, administration, and adverse effects compared to blood. We used intravital microscopy to study the microcirculation hemodynamics (arteriolar and venular diameters and blood flow and functional capillary density (FCD)) and oxygenation implications of Hemopure® administration at different Hb concentrations —4, 8, and 12 gHb/dL— compared to fresh blood transfusion during resuscitation from hemorrhagic shock. Experiments were performed in unanesthetized hamsters instrumented with a skinfold window chamber, subjected to hemorrhage (50% of the blood volume, BV), followed by 1 hour hypovolemic shock and fluid resuscitation (50% of the shed volume). Our results show that fluid resuscitation with Hemopure® or blood restored systemic and microvascular parameters. Microcirculation O2 delivery was directly correlated with Hemopure® concentration, though increased vasoconstriction was as well. FCD reflected the balance between enhanced O2 transport and reduced blood flow: 12 gHb/dL of Hemopure® and blood decreased FCD compared to the lower concentrations of Hemopure® (p<0.05). The balance between O2 transport and tissue perfusion can provide superior resuscitation from hemorrhagic shock compared to blood transfusion by using a low Hb concentration of HBOCs relative to blood.

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Section: Methodsmentioning

confidence: 99%

Resuscitation from hemorrhagic shock using polymerized hemoglobin compared to blood

Ortiz

Barros

et al. 2014

The American Journal of Emergency Medicine

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“…Normalized white blood cell (WBC) was computed similarly. Plasma Hb (due to HBOC) was measured with the B‐Hb method (Hemocue, Angelholm, Sweden; (Jahr et al , 2002).…”

Section: Methodsmentioning

confidence: 99%

Coagulation patterns following haemoglobin‐based oxygen carrier resuscitation in severe uncontrolled haemorrhagic shock in swine

Arnaud

Handrigan

Hammett

et al. 2006

Transfusion Medicine

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Massive blood loss due to penetrating trauma and internal organ damage can cause severe haemorrhagic shock (HS), leading to a severely compromised haemostatic balance. This study evaluated the effect of bovine polymerized haemoglobin (Hb) (Hb-based oxygen carrier, HBOC) resuscitation on haemostasis in a swine model of uncontrolled HS. Following liver injury/HS, swine received HBOC (n= 8), Hextend (HEX) (n= 8) or no resuscitation (NON) (n= 8). Fluids were infused to increase mean arterial pressure above 60 mmHg and to reduce heart rate to baseline. At 4 h, the animals were eligible for blood transfusions. Prothrombin time (PT), activated partial thromboplastin time, fibrinogen, thromboelastography (TEG) and platelet function analyser closure time (PFA-CT) were compared by using mixed statistical model. At 4 h, blood loss (% estimated blood volume) was comparable for HBOC (65.5 +/- 18.5%) and HEX (80.8 +/- 14.4%) and less for NON (58.7 +/- 10.1%; P < 0.05). Resuscitation-induced dilutional coagulopathy was observed with HBOC and HEX, as indicated by reduced haematocrit, platelets and fibrinogen (P < 0.05). At 4 h, PT was higher in HEX than in HBOC groups (P < 0.01). In the early hospital phase, a trend to increased TEG reaction time and PFA-CT indicates that dilutional effects persist in HBOC and HEX groups. PFA-CT returned to baseline later with HBOC than with HEX (48 vs. 24 h) following blood transfusion. At 4 h, all surviving HEX animals (n= 3) required transfusion, in contrast to no HBOC (n= 7) or NON (n= 1) animals. In this severe uncontrolled HS model, successful resuscitation with HBOC produced haemodilutional coagulopathy less than or similar to that produced by resuscitation with HEX.

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“…Hemoglobin concentration is routinely measured using automated hematology analyzers. Although, these are very accurate and reliable, they are expensive and problems of samples transport to the laboratory may delay treatment resulting in preventable deaths [7]. In poor resources where automated hematology analyzer is not available other methods of low price and that require less skill are highly needed.…”

Section: Introductionmentioning

confidence: 99%

Comparison of HemoCue® hemoglobin-meter and automated hematology analyzer in measurement of hemoglobin levels in pregnant women at Khartoum hospital, Sudan

et al. 2012

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Background Assessment of hemoglobin is one of the most reliable indicators for anemia, and is widely used to screen for anemia among pregnant women. The HemoCue ® has been widely used for as a point-of-care device for hemoglobin estimation in health facilities. Previous studies showed contradictory results regarding the accuracy of HemoCue ® . Methods This was a hospital-based cross sectional study carried- out among pregnant women at Khartoum hospital in Sudan to find out whether the measurement of hemoglobin concentration by HemoCue ® using venous or capillary samples was comparable to that of the automated hematology analyzer as standard. Bland and Altman method was used to compare the measurements with an acceptable difference of ± 1.0 g/dl. Results Among the 108 subjects in this study the mean (SD) level of hemoglobin level using HemoCue ® venous sample, HemoCue ® capillary sample and automated hematology analyzer were 12.70 (1.77), 12.87 (2.04) and 11.53 (1.63) g/dl, respectively. Although the correlations between the measurements were all significant there was no agreement between HemoCue ® and automated hematology analyzer. The bias + SD (limits of agreement) for HemoCue ® venous versus hematology analyzer was 1.17 ± 1.57 (-1.97, 4.31) g/dl, HemoCue ® capillary versus hematology analyzer was 1.34 ± 1.85 (-2.36, 5.04) g/dl, and HemoCue ® venous versus HemoCue ® capillary samples was 017 ± 1.90 and (3.97-3.63) g/dl. Conclusion Hemoglobin concentration assessment by HemoCue ® using either venous or capillary blood samples has shown unacceptable agreement with automated hematology analyzer. Virtual slides The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/8797022296725036

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The HemoCue®, a point of care B-hemoglobin photometer, measures hemoglobin concentrations accurately when mixedin vitro with canine plasma and three hemoglobin-based oxygen carriers (HBOC)

Cited by 50 publications

References 13 publications

Resuscitation from hemorrhagic shock using polymerized hemoglobin compared to blood

Resuscitation from hemorrhagic shock using polymerized hemoglobin compared to blood

Coagulation patterns following haemoglobin‐based oxygen carrier resuscitation in severe uncontrolled haemorrhagic shock in swine

Comparison of HemoCue® hemoglobin-meter and automated hematology analyzer in measurement of hemoglobin levels in pregnant women at Khartoum hospital, Sudan

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