The hemoglobins of a fresh-water teleost, Cichlasoma cyanoguttatum (Baird and Girard)—I. The effects of phosphorylated organic compounds upon the oxygen equilibria

Gillen, Robert G.; Riggs, Austen

doi:10.1016/0305-0491(71)90313-0

Cited by 20 publications

(11 citation statements)

References 17 publications

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“…High concentrations of both haemoglobin and ATP in exercising T. borchgrevinki (see Results) support this notion, by elevating the oxygen-carrying capacity of the blood and decreasing the oxyg·en affinity respectively. The important role of ATP in decreasing the oxygen affinity of fish blood was recently established by Gillen & Riggs (1971); its physiological significance is to enhance the turnover of oxygen to acti vely respiring tissues.…”

Section: Discussionmentioning

confidence: 99%

Respiratory adaptation and energy metabolism in Antarctic nototheniid fishes

Wells

1978

New Zealand Journal of Zoology

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Haemoglobin concentration and haematocrit vary considerably between four species of nototheniid fishes from Antarctica, and may be related to differences in metabolic demand. Red cell ATP and lactate were measured in the pelagic species Trematomus borchgrevinki and the benthic T. bernacchii at normal (-2.0°c) temperatures and after 7-36 h heat stress at +soc. Resting fishes have higher ATP : Hb ratios (2.0-2.4 mole/mole) at -2.0°c than do fishes at +soc (0.9-1.8 mole/mole). These ratios fall during continuous swimming, and the blood lactate level rises. The importance of ATP measurements in the control of hae'ffioglobin oxygen affinity and in relation to the theory of 'cold adaptation' is discussed.

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Section: Discussionmentioning

confidence: 99%

Respiratory adaptation and energy metabolism in Antarctic nototheniid fishes

Wells

1978

New Zealand Journal of Zoology

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“…High blood partial pressure of CO 2 (P CO 2 ) may reduce intraerythrocytic pH and reduce the affinity of Hb for O 2 by the Bohr effect, which prevents O 2 loading at the gas-exchange surface (Bohr et al, 1904). Impaired O 2 transport can also occur via the Root effect: a phenomenon found only in fishes, which prevents Hb from becoming fully O 2 saturated at high P CO 2 (Root and Irving, 1941;Gillen and Riggs, 1971). It has been hypothesized that welladapted air-breathing and amphibious fishes should possess relatively pH-insensitive Hb to reduce the consequences of Bohr and Root effects on O 2 transport (Graham, 1997;Shartau and Brauner, 2014); however, not all air-breathing fishes follow this pattern (e.g.…”

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confidence: 99%

The amphibious fishKryptolebias marmoratususes alternate strategies to maintain oxygen delivery during aquatic hypoxia and air exposure

Turko

Robertson

Bianchini

et al. 2014

Journal of Experimental Biology

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Despite the abundance of oxygen in atmospheric air relative to water, the initial loss of respiratory surface area and accumulation of carbon dioxide in the blood of amphibious fishes during emersion may result in hypoxemia. Given that the ability to respond to low oxygen conditions predates the vertebrate invasion of land, we hypothesized that amphibious fishes maintain O 2 uptake and transport while emersed by mounting a co-opted hypoxia response. We acclimated the amphibious fish Kryptolebias marmoratus, which are able to remain active for weeks in both air and water, for 7 days to normoxic brackish water (15‰, ~21 kPa O 2 ; control), aquatic hypoxia (~3.6 kPa), normoxic air (~21 kPa) or aerial hypoxia (~13.6 kPa). Angiogenesis in the skin and bucco-opercular chamber was pronounced in air-versus water-acclimated fish, but not in response to hypoxia. Aquatic hypoxia increased the O 2 -carrying capacity of blood via a large (40%) increase in red blood cell density and a small increase in the affinity of hemoglobin for O 2 (P 50 decreased 11%). In contrast, air exposure increased the hemoglobin O 2 affinity (decreased P 50 ) by 25% without affecting the number of red blood cells. Acclimation to aerial hypoxia both increased the O 2 -carrying capacity and decreased the hemoglobin O 2 affinity . These results suggest that O 2 transport is regulated both by O 2 availability and also, independently, by air exposure. The ability of the hematological system to respond to air exposure independent of O 2 availability may allow extant amphibious fishes, and may also have allowed primitive tetrapods to cope with the complex challenges of aerial respiration during the invasion of land.KEY WORDS: Hemoglobin, Oxygen-carrying capacity, Hemoglobin-oxygen affinity, Air-breathing organ, Air-breathing fish, Mangrove rivulus INTRODUCTIONThe transition from aquatic to terrestrial life represents a major step in vertebrate evolution because the physical conditions between these environments are dramatically different. Oxygen solubility is relatively low in water and even well-oxygenated aquatic environments contain only about 3% of the O 2 available in atmospheric air (Dejours, 1988). Low concentrations of aquatic O 2 , particularly in hypoxic habitats, have often been hypothesized to be one of the driving forces behind the evolution of amphibious or terrestrial life histories because invasion of land would allow animals to exploit the O 2 -rich aerial environment (Graham, 1997). RESEARCH ARTICLEDepartment of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.*Author for correspondence (aturko@uoguelph.ca) Received 4 July 2014; Accepted 15 September 2014Taking advantage of aerial O 2 presents several challenges for fishes. Water-breathing fishes exchange respiratory gases across the gills but during emersion the gill lamellae typically collapse and coalesce, reducing the surface area available for respiration. Accumulation of CO 2 in the blood of emersed fishes, resulting from the low solubility of CO 2 in...

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“…ATP and GTP have been frequently detected in the eryth rocytes of fish acting as negative modulators of the Hb-O, affinity. Other characteristic organic phos phates of the higher vertebrates such as 2,3-DPG in mammalian and IP 5 in birds, have been detected in red blood cells of some fish species (Gillen & Riggs, 1971;Isaacks et al, 1977;1978;Bartlett, 1978b;Val et al, 1992b).…”

Section: Seasonal Studiesmentioning

confidence: 99%

Respiratory characteristics of Hoplostenum littorale (Siluriformes, Callichthyidae)

Affonso

2001

Acta Amaz.

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-Hematological parameters, intraerythrocytic phosphates, hemoglobin, and whole blood Bohr effect of the South American armored catfish Hoplostenum littorale were studied during different seasons of the year. In addition, the degree of dependence on air breathing was determined for this species. The hematological parameters presented seasonal variations, which were not correlated to oxygen, temperature, and water level oscillations. Five anodic being detected, 2,3-DPG was also detected in red blood cells of H. littorale. The latter is an intraerythrocytic phosphate characteristic to red blood cells of mammalians. The increased be related to environmental temperature increase. Whole blood Bohr effect was influenced by water temperature. This study confirms H. littorale to be continuous and not obligate air breather, under all dissolved oxygen level conditions. Key-words: Adaptation, air-breathing behavior, hematology, intraerythrocytic phosphate, blood Bohr effect. Características Respiratórias de Hoplostenum littorale (Siluriformes, Callichthyidae).RESUMO -Os parâmetros hematológicos, os fosfatos intraeritrocitários, a hemoglobina e o efeito Bohr do sangue de Hoplostenum littorale foram estudados em diferentes períodos do ano. Além disso, foi determinado o grau de dependência da respiração aérea nesta espécie. Os parâmetros hematológicos apresentaram variações sazonais, as quais não foram relacionadas com as variações no oxigênio dissolvido, temperatura e nível de água. Além de ATP, GTP e Fe-GTP terem sido detectados nos eritrócitos de H. littorale, 2,3-DPG também foi detectado. Este último é um fosfato intraeritrocitário característico de células vermelhas de mamíferos. O efeito deste composto sobre a afinidade da Hb-O 2 pode estar relacionado com o aumento da temperatura. O efeito Bohr do sangue foi influenciado pela temperatura da água. Este estudo confirma que H. littorale é um respirador aéreo facultativo que utiliza a respiração aérea mesmo em condições normóxicas.Palavras-chave: Adaptação, comportamento da respiração aérea, hematologia, fosfatos intraeritrocitários, efeito Bohr do sangue.Instituto Nacional de Pesquisas da Amazônia, Coordenação de Pesquisas em Aquicultura, Av.

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The hemoglobins of a fresh-water teleost, Cichlasoma cyanoguttatum (Baird and Girard)—I. The effects of phosphorylated organic compounds upon the oxygen equilibria

Cited by 20 publications

References 17 publications

Respiratory adaptation and energy metabolism in Antarctic nototheniid fishes

Respiratory adaptation and energy metabolism in Antarctic nototheniid fishes

The amphibious fishKryptolebias marmoratususes alternate strategies to maintain oxygen delivery during aquatic hypoxia and air exposure

Respiratory characteristics of Hoplostenum littorale (Siluriformes, Callichthyidae)

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