The Hepatic Antioxidant System Damage Induced with the Cafeteria (CAF) Diet Is Largely Counteracted Using SCD Probiotics during Development of Male Wistar Rats

Abstract

The cafeteria (CAF) diet, reflective of predominant Western dietary behaviors, is implicated in hastening weight gain, subsequently resulting in health complications such as obesity, diabetes, and cancer. To this end, it is vital to notice the deleterious consequences of the CAF regimen prior to the onset of complications, which is fundamental for early intervention in the context of numerous diseases. Probiotic-derived postbiotic metabolites have gained attention for their antioxidative properties, offering a potential countermeasure against oxidative stress. This research sought to discern the protective efficacy of SCD Probiotics against liver glutathione system damage arising from the CAF diet during developmental phases. Male Wistar rats, from weaning on day 21 to day 56, were categorized into four groups: a control on a conventional diet; a group on a standard diet enriched with SCD Probiotics; a mixed-diet group comprising both CAF and standard feed; and a combination diet group supplemented with SCD Probiotics. Through the application of real-time PCR, enzyme activity assessments, and quantitative metabolite analyses, our findings highlight the CAF diet’s adverse influence on the liver’s antioxidant defenses via shifts in gene expression. Yet, the inclusion of SCD Probiotics mostly ameliorated these harmful effects. Remarkably, the positive regulatory influence of SCD Probiotics on the liver’s antioxidant system was consistently observed, independent of the CAF diet’s presence.

Supplementing probiotics during intermittent fasting proves more effective in restoring ileum and colon tissues in aged rats

“…Aging is frequently correlated with escalated lipid peroxidation, culminating in oxidative damage. 14 , 36 This heightened oxidative stress is implicated in intensifying inflammation and fostering protein modifications, subsequently leading to the generation of reactive oxygen species and promoting lymphatic infiltration, a phenomenon that aligns with our observed results. 8 , 37 Our findings reveal that both IF and SCD Probiotics effectively mitigated lymphatic infiltration in aged rats, indicating their inherent anti‐inflammatory attributes.…”

“… 10 , 11 , 12 Probiotic bacteria have exhibited efficacy in dysbiosis prevention and treatment. 13 , 14 , 15 The Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO) define probiotics as ‘live microorganisms that, when administered in adequate amounts, confer a health benefit on the host.’ 16 These include lipid profile enhancement, mycotoxin reduction, hypertension alleviation, blood glucose tolerance improvement and diabetes management. 17 Probiotics also modulate the immune response by promoting mucin production and tight junction protein expression, thereby reducing colonic inflammation and enhancing intestinal barrier functionality.…”