The hepatoprotective activity of olive oil and Nigella sativa oil against CCl4 induced hepatotoxicity in male rats

Abstract

BackgroundLiver disease is the major cause of serious health problem leading to morbidity and mortality worldwide and the problem has increased in search for hepatotherapeutic agents from plants. The present study was designed to compare the probable hepatoprotective activity of olive oil and N. sativa oil on CCl4 induced liver damage in male rats.MethodsForty males of a new model of albino rats (Wistar strain) (175–205 g) were divided into four groups. The 1st Group (G1) was the negative control group, the remaining rats were injected with CCl4 (1 ml/kg body weight) with equal amount of olive oil on the 1st and 4th day of every week for 4 weeks. The 2nd group (G2) was the positive control, the 3rd group (G3) and the fourth group (G4) were treated orally with N. sativa oil and olive oils using stomach tube.ResultsThe positive control group showed an increase in hepatic enzymes, total bilirubin, creatinine, uric acid, lipid peroxide total cholesterol, triglyceride, low density lipoprotein, very low density lipoproteins, interleukin-6, and a decrease in antioxidant enzymes, high density lipoprotein cholesterol, a decrease in total protein and albumin an when compared with negative control group. Histology of the CCl4 treated group revealed inflammation and damage of liver cells. Treating the hepatotoxic rats with olive oil and N. sativa oil showed a significant improvement in all biochemical tests compared with the positive CCl4 control group. In addition, the liver tissues of olive oil treated group showed mild improvement in inflammatory infiltration and in N. sativa oil treated group showed normal hepatocytes with no evidence of inflammation.ConclusionThis study revealed that olive oil and N. sativa oil have a protective effect against CCl4-induced hepatotoxicity in male rats. Nigella sativa oil was more effective than olive oil.

Protective effect of Trema guineensisextracts on CCl4-induced hepatotoxicity

“…On the other hand, pretreatment of rats with extracts reversed total protein and albumin reductionCCl 4 -induced. Results were consistent with Salem et al, (30) and Al-vui et al, [28] studies.Significant increase in gammaglobulins concentration is associated with liver damage caused by CCl 4 injection. Michail and Papatheodoridis [32] and Henry and Teloh [33] who revealed a high gammaglobulins level produced by reticuloendothelial liver cells during liver disease.…”

“…There was a reduction (P <0.001) in AST and ALT levels in groups treated with these extracts compared to CCl 4 -groupcorresponding to maintaining liver cell membrane functional integrity. These results are supported by previous studies plant on CCl 4 -induced hepatotoxic effect, including Corchorus olitorius [26] , Origanum elongatum [27] and Nigella sativa [28] . In addition, CCl 4 significantly decreased serum total protein and albumin inCCl 4 -untreated group compared to normal group.…”

Honey attenuates the toxic effects of the low dose of tartrazine in male rats

“…The increase in total protein and the decrease in bilirubin in G3 compared to the positive control group as a result of honey treatment is consistent with Hassan (), Afroz et al () and Al‐Seeni et al (). This amelioration with honey treatment is due to its antioxidant activity and its ability to reduce reactive oxygen intermediates that also decrease bilirubin in male rats (Al‐Seeni et al, ; Al‐Seeni, Rabey, & Zamzami, ).…”

Comparison between the Hypolipidemic Activity of Parsley and Carob in Hypercholesterolemic Male Rats

“…Other natural products contained in olive oil and Nigella sativa also shoed hepatoprotective effect against tetrachlorocarbon hepatotoxicity [40]. …”