The heterogeneity of Campylobacter jejuni and Campylobacter coli strains isolated from healthy cattle

Abstract

Aims: To identify campylobacters isolated from clinically healthy cattle at species level by a multiplex polymerase chain reaction (m-PCR). The heterogeneity among Campylobacter jejuni and Campylobacter coli isolates was also investigated by using a restriction fragment length polymorphism (RFLP) analysis of flagellin (flaA) gene. Methods and Results: Samples of intestinal contents, gall bladders, liver and faeces were collected from a total number of 1154 healthy cattle. The samples were inoculated onto Preston enrichment broth and agar. Of 1154 samples, 301 (26AE1%) were positive for Campylobacter spp. Using an m-PCR assay for species identification, 179 (59AE5%) were positive with C. jejuni specific primers while 30 (10%) were positive with C. coli specific primers. None of the liver samples examined was positive for C. jejuni or C. coli by mPCR. All the isolates identified as C. jejuni and C. coli were successfully subtyped by flaA typing. Of the 209 isolates tested, 28 different flaA types were found. Twenty-three flaA types were identified among 179 C. jejuni isolates and the remaining five from C. coli isolates. Conclusions: Although the overall results suggest that the degree of heterogeneity among the flaA genes of thermophilic Campylobacter strains isolated from healthy cattle is relatively high, they should be treated cautiously as the number of band types for C. coli was low and band type 8 in C. jejuni was represented by a high percentage (%58). Significance and Impact of the Study: The findings of the present study suggest that healthy cattle can play role in the contamination of environment and human food chain by Campylobacter spp.

Prevalence of Campylobacter spp. in Cattle in Finland and Antimicrobial Susceptibilities of Bovine Campylobacter jejuni Strains

“…This has been seen in other studies with other typing methods as well (2,6,48) and also in regard to C. jejuni isolates from chickens, sheep, turkeys, water, and human cases (9,13,38). A wide variation of SmaI subtypes could be observed among the isolates representing the most common serotype, Pen2.…”

“…in Finnish cattle at slaughter varied monthly between 18.8% and 44.1% during this 1-year study. In several studies performed in other countries prevalences of between 7% and 100% at slaughter have been reported (2,5,14,37,42,49). Due to the different study designs regarding various sampling methods and materials, detection methods, etc., the results are not always comparable.…”

Genetic Characterization of Campylobacter Jejuni and C. coli Isolated From Broilers Using flaA PCR-Restriction Fragment Length Polymorphism Method in Shiraz, Southern Iran

“…In addition, predominant flaA-typing patterns of C. jejuni strains were significantly (P < 0.05) different from C. coli isolates. Acik and Cetinkaya ( 21 ) showed that among 209 isolates, 28 different flaA types were found. Twenty-three flaA types were identified among 179 C. jejuni isolates and the remaining five from C. coli isolates.…”

Prevalence of campylobacter jejuni in meat

“…6% (4/14 beef liver) by PCR. That was higher than reported in Brazil; 1.51% (2/132 chilled beef liver)(Takeuchi et al, 2022), and Turkey; 0%(0/325 Liver samples from apparently healthy cattle by PCR)(Açik and Çetinkaya, 2005).From the other hand, our isolation rate was lower than the previous studies conducted in Washington, D.C.;15%(6/40 fresh beef liver)(Stern et al, 1984), Ottawa, Ontario; 12%(12/100)(Garcia et al, 1985), ) 33.3% then, liver (4 /14) 28.6%, and meat muscles (7/25) 28% (Table,1 and Fig.1). The fluctuation in Campylobacter species isolation rate in various studies have be attributed to many reasons as, type of examined samples, location, climate factors, hygienic measures and isolation as well as identification techniques(Jorgensen et al, 2011and Chatur et al, 2014.…”